Microbiology 197 Prepared Bacteria Gram Stains (F12) Materials required: * Microscope; clean and properly set up * Immersion oil * Lens paper * Lens cleaning fluid * Microscope drawing forms * Specimens: 1. Bacillus subtilis 2. Staphylococcus aureus. 3. Escherichia coli Procedure: 1. Observe each of slides listed in “Specimens” above. 2. Make your observations using oil immersion (1000X). 3. Using a drawing form draw the organisms
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when one gains even a very basic knowledge of how diseases are acquired and spread. The purpose of this lab was to collect and observe microbes from environmental and human body samples by culturing them on the appropriate media using aseptic transfer techniques. Materials • Distilled water • Test tube • 6 Unopened packages of 1 sterile
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first test conducted on unknown bacteria 32 was the Gram stain. From this stain‚ unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria‚ Gram-positive rods and Gram-positive spirillium. Next‚ the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green‚ steam‚ and safranin it was found that unknown bacteria 32 did not contain endospores. This eliminated Gram-positive cocci Sporosarcina
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MBK Lab 01 – Lab Report Name: ____________________ Section: ___________________ EXPERIMENT 1 TITLE: Observing Bacteria and Blood OBJECTIVE: To gain functional knowledge of microscope operations through practical applications of a microscope in the observation of bacteria and blood. PROCEDURES: Using the microscope‚ an oil immersion lens and observing Bacteria Cultures in Yogurt . Preparing a Blood Slide and observing Blood: After reviewing the section of the manual
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S. aureus is not diagnostic of the species. Some strains produce the hemolysin‚ while others don’t. 5. B. Alpha hemolytic and small. (There is a greenish color of the red blood cells around the small colonies) 6. B. Perform a Gram stain. 7. D. Gram-positive cocci in chains. 8. C. Catalase test. 9. B. Streptococcus 10. B. Optochin (P disk) 11. this test is used to differentiate different types of alpha-hemolytic streptococci. 12. A. It is viridans streptococci
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NAME: PRAJWAL BHARADWAJ Ampicillin‚ Vancomycin‚ and Tetracycline Effectiveness on Gram Positive and Gram Negative Bacteria. INTRODUCTION: Bacteria are microbial organisms which are present in various environments. Many bacteria are good and help humans synthesize materials and assist in biological processes‚ such as digestion. However‚ some bacteria can cause harmful diseases. When harmful bacteria infects the body‚ people take antibiotics in order to suppress bacteria. Different antibiotics have
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because they provide accurate data. 2. Through this letter I would like to present my opinion regarding Grams staining method and reliability of this method. According to me‚ Grams staining process is a simple technique that assists in recognition of etiological agent and therefore can be called as one of the most significant staining practices in microbiology. Though‚ Gram is concerned that the staining process developed by him is imperfect because not all bacteria can be stained by it
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Margaret E Gibson July 20‚ 2009 Microbiology Dr. Metera Lab Report 3: Labs 7 and 8- Metabolism and Biochemical Tests Abstract This experiment focused on metabolism and biochemical tests. The goal of performing these tests was to differentiate microbes from one another and to compare how metabolic and biochemical processes differ from species to species. The tests performed include: the Fermentation of Sugars Test (sucrose‚ glucose‚ and lactose)‚ the Urease Test‚ the Fermentation
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Abstract The primary focus of this lab was on microscopy and simple stains. Crystal violet and Carbol fuchsine‚ simple staining components‚ were used to stain the slide in order to see the different microbes in order to determine their cellular shape and identify unknown ones by comparing. Introduction Bacterial cells are usually colorless because cytoplasm‚ for the most part‚ is transparent. Since the bacteria are colorless‚ it is almost essential to add a stain to make the bacteria more visible
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Gram Stain: Gram is an empirical method to distinguish the species of bacteria into two groups Gram-positive and Gram-negative based on the physicochemical properties of the cells. First‚ a smear was prepared by use a sterile transfer loop that been flamed to removes some bacteria from slant agar and placed on the slide; mixed with one drop of water and let air dried. After dried‚ heat fixation the slide by passed the slide over a flame quickly 2-3 times to stick bacteria to the slide. Next‚ the
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