The Gram staining method is named after the Danish bacteriologist who originally developed it in 1882‚ Hans Christian Gram. The Gram staining process is one of the most important staining techniques in microbiology. It is almost always the first test performed for the identification of bacteria. The primary stain of the Gram’s method is either crystal violet or methylene blue‚ each almost equally effective. The microorganisms that retain the crystal violet and iodine mixture appear purplish brown
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Introduction: During this unknown lab report various test were performed to differentiate microbes from each other and to compare metabolic and biochemical process. The gram stain distinguishes between Gram positive and Gram negative bacteria based on the composition of the cell wall. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan
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Microbiology: ‘The Correct handling of Micro-organisms’ 1. Devise a title for each of the two experiments you did : (i)‚ Experiment 1 demonstrated the growth of bacteria when placed in liquid nutrient broth culture‚ the number of species present had increased in growth. .(1) (ii) Experiment 2 illustrated the growth of bacteria when placed on different surfaces of solid agar plates which included: nutrient agar‚ CLED agar and MacConkey agar; the number of species present also had increased in growth
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B. enumerate the common stains; C. differentiate between an acidic dye and a basic dye; D. compare simple‚ differential and special stains; and E. list the steps in preparing a Gram stain and describe the appearance of a gram-positive and gram- negative cells after each step.\ II. SUBJECT MATTER: Biological Techniques Topic: Staining A. Materials: * cartolina- used during the activity in lesson proper(flashcards) * iodine- used
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Chemistry Lab Report Water Hydration Introduction: A hydrated crystal or hydrated occurs when water becomes tightly attracted to a metal salt base on it’s polarity. The water molecules maintain integrity as molecules‚ however they are considered to be part of the formula of the hydrate. When the hydrate metal salt crystal is heated‚ the attractions to the water are broken by the heat energy and the water escape from the crystal. After heating the salt crystal is called as anhydrous‚ which
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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-Unknown Mixtures Lab- Purpose: The aim of this experiment was to find out the two substances of the unknown mixture from salt‚ sugar‚ dirt‚ and baking soda by looking at its properties. Hypothesis: Find the unknown substance by testing it out by heating it‚ pouring water‚ vinegar‚ and observing it . Procedure: 1) Materials- * 100ml of water * Vinegar * Bunsen Burner * 2 beakers * 1 spatula 2) First‚ do steps (3-6)to find out the properties of salt‚ sugar
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Gram Staining Gram Staining is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). Gram Staining is a way to separate one large group of bacteria into two. Crystal violet is used to dye the cells‚ which is the primary stain. Those that retain the color are grouped as Gram-positive‚ and those that do not retain the color are grouped as Gram-negative. Many of the Gram-negative bacteria are pathogenic‚ making this process useful for detecting infections
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A medical microbiology lab performs testing on human samples collected from different body sites. The tests are used to detect and identify any microorganisms capable of causing disease. Knowing of unknown microorganism is important on how this microorganism works and how it is structured‚ means knowing how it can affect humans. The purpose of this study was to identify an unknown bacterium by applying all methods that were previously conducted and learned in the medical microbiology laboratory
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Maura Gongora 12th December‚ 2014 MCB 3020L- Section 018 Unknown #16 Abstract This report contains the background information on gram positive and gram negative bacteria‚ which will aid in understanding the use of specific laboratory experiments to distinguish between the two types of bacteria. Included are the materials and methods used to identify the gram positive and gram negative bacteria and methods which also differentiate between microbes of each group. The implications of the methods of
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