• Inoculating loop • 12 sterile glass slides • Wax pencil • Igniter • Crystal violet dye • Gram’s iodine • Ethyl alcohol • Safranin dye • Paper towels • Wire rack • Sink • Brightfield compound microscope • Lens paper • Immersion oil • Pen and paper Methods I. Collecting the environmental specimens: 1. Place some distilled water into the test tube. 2. Open one package of sterile cotton swabs by peeling apart the packaging
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being altered to produce a result. There are two independent variables in this experiment which are the brand of dishwashing detergent; Palmolive‚ Morning Fresh and Ultra Coles Brand and the type of bacteria used which are; Micrococcus Luteus and an unknown bacteria from a sponge. Dependent Variable The dependent variable is the factor that is being tested which is the size of the zone of inhibition from the detergent solution and the bacteria. It will be measured in millimetres using a ruler. Constant
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Exercise 5 Introduction: Many different species of bacteria look similar under the microscope and also have the same staining results (ex. Gram stain). To be able to differentiate between the different species‚ one can look at the metabolic differences (fermentation)‚ as well as the environmental condition differences (temperature‚ pH‚ oxygen requirements). Being able to manipulate these conditions in a controlled environment can help to correctly identify the exact bacteria. Different media
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Phelps‚ Biology 205 MW 2:10pm. Unknown Organism Paper The unknown organism tested in the Mesa lab was collected from in between the toes of my puppy‚ Riley. The original culture grown consisted of dozens of visibly different organisms of varying colony colors and growth patterns. I chose to test a smaller‚ red colony for my experiments. Based on its gram reaction and oxygen requirements‚ the following tests were performed to reach my presumptive ID: Test Performed Result Gram Stain +
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Purpose: I will identify an unknown liquid by obtaining an average density & boiling point for my liquid and comparing them with the known densities & boiling points of liquids in the CRC handbook of Chemistry & Physics. I will perform these experiments using three different measuring tools (graduated cylinder‚ burette & pipette) to evaluate the precision of each one. Results: Density 1‚ 2 & 3 calculated by using graduated cylinder to obtain volume. Density 4‚ 5 & 6 calculated by using volumetric
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Bacterial Morphology Demonica Britt Microbiology DL1 March 23‚ 2013 Abstract This lab was performed to identify and familiarize with a microscope while precisely observing various bacterial shapes and their arrangements in different types of specimens of bacteria. The microscope parts and capabilities were clearly identified and used successfully and the bacteria were clearly illustrated showing the bacterial shapes and arrangements with all the appropriate magnification being utilized. Through
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Alyse Rose Microbiology Lab Bacterial Unknown March 25‚ 2013 Bacterial Unknown Report Each student was given an unknown bacteria to figure out. I was given the unknown bacteria S38. Everybody is supposed to do all sorts of test to identify the bacteria. The first thing I did was smear my bacteria on a liquid medium. I then proceeded to incubate the medium for 24-48 hours. 1. GRAM STAIN The next step I took in finding my unknown bacteria was to gram stain it. This is used to differentiate
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Objective: To successfully separate and identify all three unknown cations‚ found in a mixture and belonging to one of two specific groups (2 from one and 1 from the other). In order to do this we will be using various tests and reactions and observing the effect they had on our solution. Materials: Pasteur pipets Bunsen Burners Stirring rods Excess reagents Hot plates Sample QA unknown 101-5.7 Table of steps and observations: Step/Test |Observation |Inference | |2-1
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References: Koneman‚ E. W. (1997). Koneman ’s Color Atlas and Textbook of Diagnostic Microbiology. USA: Lippincott Williams & Wilkins. Madigan‚ et. al (2014). Brock Biology of Microorganisms (14th ed.‚ p. 78). Boston: Pearson. Mahon‚ C.‚ Lehman‚ D.‚ & Manuselis‚ G. (2011). Textbook of Diagnostic Microbiology. Missouri: Elsevier. Tille‚ P. M. (2014). Bailey & Scott ’s Diagnostic Microbiology. Missouri: Elsevier.
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1. Create and complete a data table for Part One of the lab. It should include the name of the element (or unknown) examined and the color of the observed flame 2. Identify each unknown from Part One of the lab and briefly explain why you identified each unknown as you did. Part Two (Spectroscopy): 1. Create and complete a data table for Part Two of the lab. It should include the name of the element‚ the colors‚ and the wavelength values of the observed lines. 2. Describe the line spectrum
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