7351_2006_1_S03 Rajiv Gandhi University of Health Sciences‚ Karnataka PM.D. [MICROBIOLOGY] DEGREE EXAMINATION Time: 3 Hours [Max. Marks : 100] PAPER I – GENERAL MICROBIOLOGY AND IMMUNOLOGY QP Code: 7351 Your answers should be specific to the questions asked. Draw neat labeled diagrams wherever necessary. Answer all questions LONG ESSAYS 1. 2. 2 X 20 = 40 Marks Discuss the production‚ properties and uses of monoclonal antibodies Discuss various in vitro antibiotic sensitivity
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Dr. GEORGE ROMBO Cholera This is an infection of the small intestine caused by bacterium vibrio cholera. Transmission is mostly from the fecal contamination of food and water that is caused by poor sanitation Susceptibility About 100 million bacteria must typically be ingested to cause cholera in a normal healthy adult. Children are also more susceptible with 2- 4 year old having the highest rates of infection Individual susceptibility to cholera is also affected by their blood type with
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A medical microbiology lab performs testing on human samples collected from different body sites. The tests are used to detect and identify any microorganisms capable of causing disease. Knowing of unknown microorganism is important on how this microorganism works and how it is structured‚ means knowing how it can affect humans. The purpose of this study was to identify an unknown bacterium by applying all methods that were previously conducted and learned in the medical microbiology laboratory
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Identifying an Unknown Aldehyde or Ketone Introduction The purpose of this lab is to identify an unknown aldehyde or ketone substance using chemical tests. The chemical tests used in this experiment are solubility‚ Schiff‚ Bisulfite‚ and Iodoform tests. Also‚ a 2‚4-dinitrophenylhydrazone derivative synthesis reaction will be completed from which a melting point will be obtained. The chemical test results and the melting point analysis will be compared to the table of compounds given to find the
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METHODS In this experiment‚ plasmid lux and a control plasmid (pUC18) will be introduced into E. coli by transformation. There are four basic steps to the procedure; Preparation of competent cells (These steps should be performed by the instructor.) 1. Place a vial of CaCl2 solution and the tube of E. coli in the ice bath. 2. Using a sterile pipet‚ transfer 590µL CaCl2 solution to the tube containing 50µL of the bacteria. 3. Tap the vial with the tip of your index finger to mix the solution. 4. Incubate
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Having the ability to collect and identify unknown microorganisms is vital in health and medicine. This capability is important for a variety of reasons‚ such as knowing the causative agent of disease‚ knowing if the microorganism obtains any beneficial properties and knowing the correct microorganism to use to create a successful antibiotic. Implementing the experimental methods learned thus far in the microbiology laboratory allowed an unknown bacterium to be identified as a result of this study
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which organism we had in our unknown mixed culture tube by running a series of experiments to detect which specific Gram negative organism we had. To detect your gram positive from the mixed culture was given as extra credit points also. A Gram stain was performed and isolation streak plate in order to isolate and observe the unknown organism. Before the series of test‚ a dichotomous key had to be written up in order to know what steps and tests to run to identify the unknown Gram negative organism. I
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CHAPTER I INTRODUCTION 1.1 Background Food is one of human sources of calorie‚ protein‚ fats‚ and nutrition. Yet‚ because of the highly nutritious content‚ food is susceptible to growth of microorganisms. By the presence of microorganisms in food‚ the food is more likely to have shorter shelf life. Thus‚ mostly it is resolved by the addition of antimicrobial substances to food‚ such as condiments and preservatives. Condiments and preservatives could inhibit the growth of microorganisms
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Bibliography: 13.1 Books * Electronics Commerce ----- Henry Chan * Software Engineering ----- Roger Pressmen * E-Business Organizational and technical foundation----- Wiley Publication 13.2 Websites * www.famecinemas.com * www.imdb.com * www.w3schools.com * www.webtemplate.com * www.asp101.com
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virus too small to be seen without a microscope (smaller than 0.5mm) Misconceptions of Microorganisms * All microorganisms are pathogenic (disease producing) FALSE * The majority of bacteria are harmless and beneficial TRUE Aspects of microbiology * Basic biological science * About living cell and how they work * About microbial diversity and their evolution * Applied biological science * What microorganisms do; their role in the nature? * Causative agents
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