ABSTRACT: Availability‚ low price‚ and high degree of reduction have made glycerol a highly attractive and exploited carbon source for the production of fuels and reduced chemicals. Here we report the quantitative analysis of the fermentative metabolism of glycerol in Escherichia coli through the use of kinetic modeling and metabolic control analysis (MCA) to gain a better understanding of glycerol fermentation and identify key targets for genetic manipulation that could enhance product synthesis
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Student Lab Section 6 E. Coli Genetic Transformation with pGLO Plasmid Introduction: Genetic transformation is where one organism takes on a characteristic from another organism (Bacterial Transformation 2013). For this experiment we used the bacteria E. Coli to take in foreign jellyfish DNA which will allow it to change genetic material. This experiment determines the effects that the plasmid pGLO has in transferring the Green Florescent Protein found in a jellyfish into the bacteria. It
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References: 1. Beishir‚ L.: Microbiology in Practice: A Self-Instructional Laboratory Course‚ Fifth Edition. (Harper Collins: New York) 1991. 2. Jawetz‚ Melnick and Adelberg: Medical Microbiology‚ Nineteenth Edition. (Appleton and Lange: Norwalk‚ CT) 1991. 3. Tortora‚ Case and Funke: Microbiology: An Introduction‚ Fourth Edition. (Benjamin Cummings: Redwood City‚ CA) 1992. 4. Zubay: Biochemistry. (Addison Wesley:
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Identification of Unknown Report Abstract: The purpose of this lab was to determine the identity of an unknown bacteria slant culture. The unknown was identified using differential tests to determine its metabolic and morphology characteristics. Results from the differential tests indicated that unknown culture 5 is Staphylococcus aureus. Introduction: The purpose of this lab was to determine the identity of an unknown bacteria slant culture using a series of differential tests. The tests
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isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question and how the bacteria function. Materials and Methods Over a two week period‚ eight prepared types of test media were provided to identify the assigned unknown mixed cultures. Not
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Microbiology: ‘The Correct handling of Micro-organisms’ 1. Devise a title for each of the two experiments you did : (i)‚ Experiment 1 demonstrated the growth of bacteria when placed in liquid nutrient broth culture‚ the number of species present had increased in growth. .(1) (ii) Experiment 2 illustrated the growth of bacteria when placed on different surfaces of solid agar plates which included: nutrient agar‚ CLED agar and MacConkey agar; the number of species present also had increased in growth
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Gbengon‚Sennie Microbiology Lab Case study # 5 November 1‚2012 1. What evidence suggests that the young graduate student has contracted what disease? Ans: The signs and symptoms the young graduate student presented‚ show evidence of Lyme disease. 2. What is the environmental pathway for the vector of this disease? Ans: The environmental pathway for the vector of this disease would be a forested habitat. The black-legged tick or the deer tick is the principle vector of this disease (Lyme
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Unknown Lab Report April 25th‚ 2006 Introduction The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures
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Morales‚ Marinel M. Dr. Annie Cu Gallardo BSE-Biological Sciences III-3 Microbiology (Lecture) Microbiology Written Report REPORT NO. 1 Prokaryotes: Bacteria Most of us have been conditioned to think of bacteria as invisible‚ potentially harmful little creatures. Actually‚ relatively few species of bacteria cause disease in humans‚ animals‚ plants‚ or any other organisms. In fact‚ all organisms made up of eukaryotic cells probably evolved from bacteria-like organisms‚ which were some
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we have learned in the lab to identify two unknown organisms. PROCEDURE I was given unknown #76. I performed the streak plating method with my unknown organisms. To perform this exercise I needed a TSA plate and labeled the bottom of it with my name‚ group number and organism. I also divided the bottom into three sections. After that‚ I sterilized my inoculating loop using the bunsen burner flame. After letting the loop cool‚ I reached into my unknown test tube with my loop and grabbed the organisms
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