Funke‚ Christine L. Case (2010). Microbiology: an introduction (10th ed.). San Francisco‚ CA: Pearson/ Benjamin Cummings. James G. Cappucino‚ Natalie Sherman (2011). Microbiology: a laboratory manual (9th ed.). San Francisco: Benjamin Cummings. Micrococcus luteus. (n.d.). Retrived Jun 11‚ 2012 from the Wikipedia: the free encyclopedia: http://en.wikipedia.org/wiki/Micrococcus_luteus. ----------------------- Sediment Confluent growth Single colony
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Materials: 1 clean microscopic slide‚ 3% H2O2 solution‚ swabs. Micrococcus luteus‚ Enterococcus faecalis‚ patient G Procedure: 1) Scrape some cells off from each bateria to the slant and place them on glass slide. 2) Place one or two drops of H2O2. Watch for bubbling as an indication of O2 production. 3) Discard the used slide container. Results: Organisms Bubbles formation Catalase Patient G Bubbles Positive Micrococcus luteus Bubbles/O2 formed Positive Enterococcus faecalis No bubbles/No
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Department of Food Science and Technology College of Agriculture and Food Science Visayas State University Baybay City‚ Leyte Microbiology of Fish and Fish Products Introduction: Fish is a major staple food in most parts of the world and are second only to meat as the major animal protein in most diets. Whilst foods such as ‘meat’ form relatively well -defined groups of raw materials‚ ‘fish’ constitutes a large range of types (some sources recognise over 20‚000 identified species)‚ caught over
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04/29/2013 G-Unknown code Gram Positive-Micrococcus luteus Gram Negative- Klebsiella pneumoniae Unknown Paper The unknown project was a very good realization of me and my partner going out by our selves. The very first day of the Unknown project I was acquainted with Denise who was very friendly and was very nice as far as assisting me in the project. The first day we were introduced too various different forms of the unknown such as broth‚ Blood agar plate‚ MSA plate
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Staphylococcus has many species‚ so test focused on differentiating the many species of Staphylococcus were conducted. First‚ to confirm that the unknown wasn’t a Micrococcus sp.‚ the bacitracin test was used. This test confirmed that the unknown 32 was a resistant bacitracin resistant bacterium. Since Micrococcus spp. are susceptible bacteria‚ Micrococcus spp. were eliminated. The next test was the coagulase test which tests for the enzyme coagulase. In the presence of coagulase‚ fibrinogen in blood is converted
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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PROCEDURE: Part A (Effect of temperature on growth) 1) 15 tubes of glucose broth are provided and one set of 3 tubes are inoculated with each of the following cultures; Escherichia coli‚ Pseudomonas fluorescens‚ Micrococcus luteus and Saccharomyces cerevisiae. The last served as control. 2) One of the three tube of each culture is incubated at each of the following temperature: * 4°C * 37°C * 55°C 3) All the tubes are incubated within 5 minutes after inoculating. The turbidity
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Year 12 Nutrition Stage 2 SACE: Practical – Manipulation of Microbial Growth | August 25 2013 Hypothesis: The hypothesis for this particular practical experiment is that‚ as the pH decreases or increases from neutral pH of approximately 7‚ the amount of microbial growth on the agar solution will decrease Materials: Agar Solution Preparation: *hand sanitizer/ hand wash soap *small container *1g of agar powder *0.25g of beef extract stock *60mL of heated water *electronic balance *small
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making it unavailable. Anaerobes will grow in this medium. Aerobes can also grow but only in the upper layers of this medium. PURPOSE To identify bacteria based on growth in oxygen at differing levels. CULTURES Pseudomonas aeruginosa or Micrococcus luteus‚ Clostridium‚ Escherichia coli (Aerobes) (Anaerobe) (Facultative anaerobe) MEDIA 3 TSA plates (for anaerobic chamber) CULTURE CONDITIONS • Lightly streak 4 quadrants on TSA agar for anaerobic
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help the body lyse‚ or break down bacteria by targeting peptidoglycan in bacterial walls. The solutions and fluids studied were saliva‚ mucus‚ tears‚ a stock solution of lysozomes‚ and distilled water. The solutions were placed in agar containing Micrococcus Luteus and we observed the amount of bacteria that was lyzed around them. The measurements were taken by observing where the agar cleared around the solutions‚ as the agar was cloudy where bacteria was present. I hypothesized that saliva would
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