Drugs and chemicals Sitagliptin (Sita) was manufactured by Merck Sharp & Dohme Ltd (Pavia‚ Italy)‚ dissolved in saline. Gentamicin sulfate was obtained as ampoules from Memphis Co.‚ for Pharm. and Chem. Ind. (Cairo‚ Egypt). Other used chemicals were purchased from Sigma Chemical Co. (St. Louis‚ MO). Animals Animal procedures and care were in accordance with the National Institute of Health guide for the care and use of Laboratory Animals (NIH Publication No. 85-23‚ revised 1996) and were approved
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genetics to the analysis of evidence found at crime scenes or on/in the bodies of crime suspects. The field is a combination of criminalistics and analytical toxicology. Criminalistics is the qualitative examination of evidence using methods such as microscopy and spot testing‚ whereas
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Chapter 4 Microscopes Two important factors in microscopy are: 1) Magnification: an increase in the object’s apparent size compared with its actual size. 2) Resolving Power: the ability of an optical instrument to show two objects are separate. Three types of microscopes: 1) Light Microscope (LM) M: 1000x RP 0‚2 micrometer (small bacterial cell) 2) Electron Microscope (EM) uses a beam of electrons to resolve electrons‚ better resolving powers than light microscope M:100‚000x RP 0
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intentionally blank 27 Laboratory 2: Scientific Methodology & Enzyme Activity 28 In-Lab Report Sheet 28 Objectives 33 Introduction 33 Parts of the Swift M5 Microscope 33 Part A: Using the Swift M5 Microscope and Viewing a Letter “e” 34 Lab 3: Microscopy 39 In-Lab Report Sheet 39 Part A: Using the Swift M5 Microscope and Viewing a Letter “e” 39 Laboratory 4: Cell Structure & Function 43 Pre-Lab Assignment 43 This page left intentionally blank 44 Lab 4: Cell Structure
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of the stool specimen is collected and placed on the slide containing the normal saline‚ and is emulsified with it. After emulsification‚ the slide is covered with a cover slip and allowed to stand for 30 seconds to a minute and examined under a microscope using both low and high magnifications(x10 and x40). It was noticed that the number of parasite eggs determine the degree of infectious parasitemia that could result. Concentration of the stool specimen allows for easy viewing of hidden micro
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Biochemistry and Molecular Biology Mitochondrial Oxidative Stress in the Retinal Pigment Epithelium Leads to Localized Retinal Degeneration Haoyu Mao‚1 Soo Jung Seo‚1 Manas R. Biswal‚1 Hong Li‚1 Mandy Conners‚1 Arathi Nandyala‚1 Kyle Jones‚1 Yun-Zheng Le‚2 and Alfred S. Lewin1 1 Department of Molecular Genetics and Microbiology‚ College of Medicine‚ University of Florida‚ Gainesville‚ Florida‚ United States Departments of Medicine‚ Endocrinology‚ and Cell Biology and Harold Hamm Diabetes Center
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majors. It covers some central concepts in biology. Topics include molecules‚ cells‚ enzymes‚ photosynthesis‚ cellular respiration‚ cellular reproduction‚ genetics‚ and biotechnology. The laboratory includes basic laboratory skills such as safety‚ microscope use‚ and measurement‚ and it reinforces topics discussed in lecture. This course meets the SUNY General Education course requirements for natural sciences. Credit Hours: 4; Contact Hours: 5 Instructor Dr. Michael Gregory
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that pieces of cork viewed under the microscope presented small cavities similar to pores which were filled with air. Based on later knowledge‚ of what were the walls of those cavities constituted? What is the historical importance of that observation? The walls of the cavities observed by Hooke were the walls of the plant cells that form the tissue. The observation led to the discovery of the cells‚ a fact only possible after the invention of the microscope. In that work‚ Hooke established the
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die used for bioreplication. Optical microscopy of the dorsal side of the nano-bioreplicated A. planipennis decoy confirmed the similarity in the surface structure of the beetle which is colored by metallic paint. For natural Agrilus planipennis: Dorsal view optical microscopy images revealed the surface structure of the beetle elytron. The spines and cilia were revealed by the higher magnification images of optical microscopy. Scanning electron microscopy images revealed shows distinct patterns
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Unit 1 Contents Specification Biological Molecules Cells Human Physiology Disease Appendices Chemical bonds Carbohydrates Lipids Proteins Biochemical Tests Enzymes Eukaryotic Cells Prokaryotic Cells Cell Fractionation Microscopy The Cell Membrane Movement across Cell Membranes Exchange The Gas Exchange System Lung Diseases The Heart Coronary Heart Disease The Digestive System Cholera Lifestyle and Disease Defence against Disease Immunisation Monoclonal Antibodies
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