Title: preparation of films for staining Objective: to produce a thin smear of bacteria adhering to a clean microscope slide as preparatory to staining. Procedure: A. From broth cultures 1. Inoculating loop was sterilized using Bunsen burner and let to be cool before use it to obtain bacterial suspension from the tube. 2. The bacterial then placed in the center of the clean microscope slide. It also speared to produce thin films. 3. The films are set to air dry before wafting the slide gently
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Lab 2: The Metric System and Microscope Name _________________________ Date __________________________ Section 1: The Metric System The Metric System is known as the International System of Measurement. The Metric System is based on Standard-prefixes that correspond to ‘multiples of 10’. The measurements of volume (the liter)‚ mass (the gram)‚ and length (the meter) are the baseline for all metric unit conversion factors. In the United States‚ the English System is more commonly used;
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OBSERVATION OF BACTERIAL CELLS VIA MICROSCOPE In this experiment‚ we have learned several basic molecular biology techniques. Here they are; Simple staining: Only one type of dye used for simple staining and it stains all cells the same color. Creating contrast between cell and background to observe cell morphology is aimed. Differential staining: A differential stain uses more than one dye and stains different kinds of organism with different colors. Positive staining: If cell itself is stained
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that gives strong circumstantial evidence for the particulate nature of air. Materials: ✓ Smoke cell‚ incorporating a light source and lens (Whitley Bay pattern) ✓ Microscope‚ low power (e.g. x10 objective‚ x 10 eyepieces) and large aperture ✓ Power supply‚ 0 to 12 V dc ✓ Microscope cover-slip ✓ Smoke source (e.g. paper drinking straw) Setting up apparatus: [pic] The smoke can come from a piece of burning cord using a dropping pipette or
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their structure is linked to the development of microscopes‚ which allowed scientists to observe microscopic cells. In the mid 1600s‚ in the Netherlands‚ the scientist Antonie van Leeuwenhoek developed the first known microscope using a single magnifying lens. He is described as the first microbiologist because he was the first to observe microscopic cells that we now know to be bacteria and blood cells. Van Leeuwenhoek shared the designs of his microscope‚ as well as his observations‚ with the scientific
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instruments such as microscope‚ staining‚ stethoscope and X-rays. Of the five senses‚ the most important is sight. It aids in the process of gathering information about the environment that we are part of. However‚ this visual gathering is adequate only to a certain point. Beyond this point‚ the unaided human eye fails to help us; the amount of detail that it can provide is severely limited. In order to overcome this limitation‚ humans started to develop instruments like microscope. The development
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experiment is conducted so that we may know how things happen in the level of cells. Objectives: 1. To demonstrate the principle of Osmosis and to apply it with actual life situations. Materials and Methods: • Materials: Compound microscope Glass Slide Cover Slip Tissue Paper Blade Rhoeo discolor Leaves Salt Solution • Procedures: Place a drop of distilled water on a clean glass slide. With the use of a blade‚ cut a thin slice of the lower epidermal leaf
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KARYOTYPING Karyotyping refers to use of a microscope to examine the size‚ shape‚ And number of Chromosomes in a sample of body cells. Extra‚ missing‚ Or abnormal positions of chromosome pieces can cause problems with a persons growth‚ development‚ and body functions. In karyotyping‚ the chromosomes in a cell are stained with a dye to make the bands of each chromosome visible. Banding helps show differences in structure among the chromosomes and helps arrange them into pairs. The chromosomes
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sample mounting The paraffin section was cut at 5 μm of thickness with a rotary microtome. Sections of tissues obtained were floated on the surface of warm water in a floatation bath to flatten the sections. The sections were picked up onto the microscope slide‚ drained it in vertical position for several minutes. The slides were then placed on a slide holder in the oven at 37 °C overnight. Step 5: Staining The slides were placed in a staining rack and were dipped into xylene for 5 minutes
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PRACTICAL 3 QUESTIONS PART 1 Q 1. What is the diameter of the field of view of your microscope using the x10 objective (i.e. at a magnification of x100)? The diameter of the field of view using the x10 objective is 1800 µm. Q 2. What is the diameter of the field of view of your microscope using the x40 objective (i.e. at a magnification of x400)? The diameter of the field of view using the x40 objective is 400 µm. Q 3. What is the ratio of magnification between the x10 and x40 objectives?
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