Laboratory Report: Observing Bacteria Purpose: Is to gain knowledge on how to use a microscope and oil immersion lens while observing prepared bacterial slides and also to prepare slides for observable bacteria culture from yogart. Procedure: Exercise 1: Using the Microscope - Viewing Prepared Slides I started out by reading and reviewed the science lab safety reinforcement Agreement and the instruction to oil immersion lens. After setting up my lab station I view the six prepared slides
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carbohydrates in starch suspension and potato cell. If carbohydrate is present in starch suspension‚ the solution will turn into dark blue when iodine solution is added. If carbohydrate is present in potato cell‚ starch granules can be seen clearly under microscope when iodine solution is added. The second experiment is to test the existence of lipids in margarine and olive oil. If a lipid is present in margarine‚ the solution suspended with alcohol will turn cloudy when it is mixed with distilled water. If
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The effect of Sodium Chloride (NaCl) salt concentration on osmosis in potato cells The movement of substances in plant cells involves many processes and systems‚ all of which may affect the plant bio-chemically and physically‚ and one of these processes is osmosis. Osmosis is the flow of water through a semi-permeable membrane of a cell moving from an area of higher water potential to an area of lower water potential until reaching equilibrium known as isotonic. Before reaching the point of being
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and it is easier to observe the heart rate of the Daphnia through the microscope. [Figure 1] Daphnia Variables Independent variables: Same volume different concentration of caffeine Dependant variables: Heart rate of Daphnia Controlled variables Time Temperature Size of Daphnia Method Equipment Cavity slide Small piece of cotton wool Pasteur pipette Caffeine at different concentrations Microscope Tally counter A piece of wool is teased and made into a small loop
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Ceratopteris richardii: The effects of increasing spore density in detecting higher percentages of sexually expressed gametes. Abstract: The Ceratopteris genus is a model plant system in allowing the study of life and reproductive cycles of spores‚ allowing for spore to gamete cellular and physical observation. The triangle water fern‚ Ceratopteris richardii‚ provided for visual identification of this transition. Spores of different densities were inoculated‚ cultured‚ and observed in germination
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that the weight decreases. In our second experiment‚ we use three slices of aquatic plant Elodea and add pond water one leaf‚ distilled water for the second and 20% NaCl for the third‚ then observe the cells of each leaf with a compound microscope. Under microscope‚ chloroplasts (green organelles) in Elodea tend to bound to the cell wall in pond water‚ spread all over the cell in distilled water‚ pull away from cell wall in 20% NaCl. In our last experiment‚ we use a dialysis bag that contains 30%
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gametes. This process is important for generating diversity in life and aiding in evolution. Materials and Methods The materials used in the lab were two agar petri plates‚ a permanent marker‚ labeling tape‚ sterile scalpel‚ compound microscope‚ toothpick‚ microscope slide‚ water‚ cover slip‚ and the ascospore Sordaria cultures‚ which included wildtype (dark)‚ tan‚ and grey (mutant) fungi. First‚ collect two agar plates to make the cross between the three different colored ascospores. The Sordaria
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Abstract Stomata is important to plants because of their role in gas exchange and photosynthesis. Clear nail polish and clear plastic tape were used to make leaf impressions of A. floridanum. Once impressions were completed‚ compound microscopes were used to count the total number of stomata per slide. The counts of stomata were used to find stomatal densities‚ along with the open/closed ratios of stomata. Stomatal density and open/close ratio of stomata did not differ between the two locations
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B.Sc. First Year Semester – I Paper I .Fundamentals of Microbiology Credit - 1 1 Scope & relevance of Microbiology i) ii) iii) Definition & concepts Types of microorganism Distribution of microorganisms in nature 2. Development of Microbiology as a Scientific Discipline 1.1 History i) ii) iii) iv) v) vi) Credit – 2 Early observation of microorganisms Spontaneous generation conflict : Contribution of scientists Recognition of the microbial role in diseases. Koch’s postulates Recognition of microbial
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separate when seen by microscope. Mounting press was use to mount the specimen to make it suitable for handling at the time of grinding and polishing. Different kind of sand paper was used to grind and make smooth the spacmens and finally polishing cloth with diamond suspension was used to remove the extra scrachs . to diffrantiate the grain boundaries it was etched by nital solution. Finally the first observation was taken made by optical microscope and scanning electron microscope.
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