Cellular and Molecular Techniques - Lab #1 BIO 349 Lab Report #1 Microscopy and Staining Abstract The primary focus of this lab was on microscopy and simple stains. Microscopy that was used were magnification‚ slide preparation‚ and staining. Methylene blue‚ a simple staining component‚ was used to stain the slide in order to see the different microbes and determine their cellular shape. Introduction The purpose of this lab was to become familiar with the light microscope and how
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Experiment 2: Microscopy & Simple Staining September 30‚ 2013 General microbiology laboratory The invention of the microscope has opened up a whole new dimension in science. By using microscopes scientists were able to discover the existence of microorganisms‚ study the structure of cells‚ and see the smallest parts of plants‚ animals‚ and fungi. Today‚ the microscope is still a commonly used tool to diagnose illness in hospitals and clinics all over the world (1)
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1. Introduction Microscopy is an essential technique not only in cell biology but natural science as a whole. We compared different types of microscopic techniques ‚ according to the specimen used and the scope of the experiment. Two specimens‚ stained and unstained‚ containing CHO cells‚ were prepared‚ examined and analyzed under the microscope using bright field (HF)‚ dark field (DF) and phase contrast (PH) settings. In addition‚ the four phases of cell division cycle were estimated.. Bright
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By examining blood smear by microscopy it can detect infections whether they are viral or bacterial. Blood smears are examined by looking at the white and red blood cells appearances. The aim of the experiment is to compare a normal blood smear and its pathological features to those with an infection or abnormality by the use of microscopy looking at the cells involved with blood. A normal blood smear would contain 3 to 4 white blood cells per field in microscopy. If there is an increase in the
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Virtual Lab 1: Virtual Microscopy A. Estimate the size (length and width) of these microscopic objects in micrometers (microns): 1. An E. Coli cell. 3x 0.6 μm = 1.8 μm 2 A mitochondrion. 4x 0.8 μm = 3.2 μm 3. A Red blood cell. 8 μm 4. A virus. 220 nm = 0.00022 μm 5. A water molecule. 275 pm = 0.000275 μm B. 1 Describe three differences between prokaryotic and eukaryotic cells. The three differences between prokaryotic and eukaryotic cells are: Eukaryotic cells contain a nucleus inside
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Gram Staining Lab Introduction Gram staining is a very important technique used in biology labs all over the world. It is a technique used to differentiate types of bacteria using certain physical and chemical characteristics of their cell walls. Gram positive bacteria (which show up purple after the gram staining process) have a very thick layer of peptidoglycan where gram negative bacteria (which show up pink after the gram staining process) have a much thinner layer of peptidoglycan. One thing
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Introduction Gram staining was developed by Christian Gram in the 1800’s‚ a Danish bacteriologist. (Smith and Hussey‚ 2005) It was the first differential staining technique and most common used in microbiology. Furthermore‚ bacteria are transparent and cannot be seen through the microscope. For that reason‚ Gram staining is an important tool for distinguishing between two main types of bacteria Gram-positive and Gram-negative. The Gram stain differentiates the Gram positive and gram-negative on
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Michele Hindmarsh mhindma@my.wgu.edu Student ID# 000383032 MLT1 – Experiment 5; Task 6 Differential Staining Heidi Atkinson‚ MS Lab Experiment #5-Differential Staining Through the process of differential staining‚ there are distinct differences between the cell walls of gram-positive and gram-negative bacteria. In the case of gram-positive bacteria‚ the cell wall is comprised of 60-90% peptidoglycan and is very thick. There are numerous layers of teichoic acid bound with peptidoglycan
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Endospore and Capsule Cell Staining Allison Lui Partner: Mary Chagin BIOL-235 W07 Dr. Runco 2/10/15 Introduction The purpose of this lab is to learn what endospore and capsules are‚ and how to identify them under a microscope using capsule and endospore staining methods. Capsules are found only in select bacteria‚ and serve a protective purpose. Made out of sugars and proteins‚ they are antiphagocytic‚ which prevents other cells to engulf the bacteria through phagocytosis. It
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Lab #1 ‐ Introduction to the Microscopy & Observation of Prokaryotic and Eukaryotic Cells Introduction Many of the cells and organisms that you will be studying are at the lower limits of visibility of light microscopes; therefore‚ it is extremely important that you attain critical lighting and focussing. It is also important to handle the microscope competently to avoid damaging either the microscope or the preparation you are studying
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