Objective : To extract the DNA from onion samples. Introduction : Nuclues has DNA molecule in it. It is packaged into thread like structure known as chromosomes. Each and every chromosome is made up of DNA tightly coiled many times around proteins which is known as histones. Histones supports the structure. Chromosome cannot be visible even under the microscope if the cells are not dividing. DNA consist of 2 long polynucleotide which is composed of 4 types of nucleotide units. Nucleotides
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The history of DNA structure discovery(sec.4.1): 1869- Johan miescher •studies the nuclei of white blood cells(isolated th material using HCL and digestive proteins •Named the substance nulclein and also found the material was rich in nitrogen and phosphorus. 1919-Pheobus levene •Discovered that DNA was made of chains of nucleotides *see nucleotide structure* 1920 DNAvsRNA * see chart •Thought that 4 nucleotides were connected in the same repeated pattern •protein gas 20 amino acids
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DNA Transcription The process of transcription is where a copy of a gene is made within DNA to use as RNA. It is located in the nucleus of eukaryotes and in the nucleoid of prokaryotes. DNA stores information encoded in a genetic code. The code consists of four letters and they are T (thymine)‚ G (guanine)‚ A (Adenine)‚ and C (cytosine). One gene codes for on protein. RNA is a molecule that copies information that is coded in another genetic code. This code also consists of the same four
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Recombinant DNA Report Our final annotated gel image sums up the successful experiments we performed over the course of 8 weeks. The image will be referred to throughout the report: Lane 1: 10 µL of ladder. Lane 2: 20 µL of a pAMP- EcoRI/HindIII double digestion. Within the double digestion‚ one can find 8 µL of pAMP‚ 1 µL of the EcoRI enzyme‚ 1 µL of the HindIII enzyme‚ 5 µL of 10x Buffer 2.1‚ and 35 µL of water. A total volume of 50 µL was present
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Associate Program Material DNA Worksheet Answer the following in at least 100 words: 1. Describe the structure of DNA. DNA is a polymer‚ which is a chemical compound or a mixture of compounds consisting of repeating structural units. These repeating structures are created through polymerization. The monomer‚ meaning one part‚ units of DNA are nucleotides. Each nucleotide consists of a five carbon sugar‚ also known as deoxyribose‚ and nitrogen containing base attached to the sugar
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life (1). Significant developments have been made in gene monitoring techniques specifically in DNA microarrays which only very recently revolutionized genome expression analysis (1). Despite continuous improvements and modification to the technique‚ DNA microarrays are still no more than a glass microscope slide studded with individual immobile nucleotide fragments (1‚ 2). The fundamentals of DNA microarrays are set on complementary base-pairing (3)‚ and because the exact sequence and position
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Forensic DNA Evidence DNA has become one of the most accurate tools used in law enforcement in determining guilt or innocence. DNA is different in all people it is our “genetic blueprint.” DNA is so significant to law enforcement because DNA left at a crime scene can be collected and tested to see if there is a match. It is unique because it ensures accuracy and fairness. The initial use of DNA began in Britain in 1986; the FBI used it for the first time in the United States two years
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DNA‚ or deoxyribonucleic acid‚ is the hereditary material in humans and almost all other organisms. Nearly every cell in a person’s body has the same DNA. Most DNA is located in the cell nucleus (where it is called nuclear DNA)‚ but a small amount of DNA can also be found in the mitochondria (where it is called mitochondrial DNA or mtDNA). The information in DNA is stored as a code made up of four chemical bases: adenine (A)‚ guanine (G)‚ cytosine (C)‚ and thymine (T). The order‚ or sequence‚ of
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Roles of DNA and RNA in the Human Body and Medicine Anatomy and Physiology 1 Dr. Joy Henry Schonathan Crews 3/20/2015 Roles of DNA and RNA in the Human Body and Medicine Introduction Deoxyribonucleic acid (DNA) is the foreman of the body with a strict set of blueprints for what needs to be done in an organism’s cell and how.1 Each cell is encoded with a specific sequence of DNA which stores how it is to be made and reproduce. Ribonucleic acid (RNA) is the assistant to the foreman
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DNA Packaging: Nucleosomes and Chromatin By: Anthony T. Annunziato‚ Ph.D. (Biology Department‚ Boston College) © 2008 Nature Education Citation: Annunziato‚ A. (2008) DNA packaging: Nucleosomes and chromatin. Nature Education 1(1) Each of us has enough DNA to reach from here to the sun and back‚ more than 300 times. How is all of that DNA packaged so tightly into chromosomes and squeezed into a tiny nucleus? The haploid human genome contains approximately 3 billion base pairs of DNA packaged
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