(Appendix A). 1mL of the dye solution was added to 20uL of the protein sample‚ mixed and incubated for 10 minutes at room temperature before measuring the absorbance at 595nm using the spectrophotometer. 3.6 Verification for the Homogeneity and Molecular Weight of the DNA Polymerase
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nucleic acids and proteins. Electrophoresis can take a mixture of macromolecules of different molecular weights‚ shapes‚ and various electrical charges to determine all the various compounds in the mixture and allowing for further purification that can aid in details of individual elements of the mixture being studied. Agarose gel electrophoresis is a very important technique used in the field of molecular science. The field of forensics utilizes agarose gel electrophoresis by allowing investigators
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Kris Lukas Career Journal Name: Biochemist Education and Training -Candidates should have a high school diploma with good grades in chemistry and biology -A Bachelor’s degree in chemistry is good to have as well as a Doctoral degree -Good to have a Major’s degree in biology‚ chemistry‚ or biochemistry - Should have between 18-24 months of experience working as a biochemist in a research facility or as an assistant Responsibilities -A typical work day includes studying
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vector was used to clone the individual PCR products which then transformed into E. coli. Catalytically active domains were studied by analyzing deduced amino acid sequences of genes. SDS-PAGE analysis was carried out to estimate the molecular weights of proteins. Molecular weights of peh‚ celB and celC products were found to be 41.5 kDa‚ 29.5 kDa and 40 kDa‚ respectively. Agar diffusion assays were performed to qualitatively determine the activities of polygalacturonase and cellulase of the cloned
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Characterization of a Yam Class IV Chitinase Produced by the Recombinant Pichia pastoris X-33 Department of Botany‚ Jahangirnagar University‚ Savar‚ Dhaka 1342‚ Bangladesh Abbreviations: Endo H‚ Endoglycosidase H; PR protein‚ pathogenesis related protein; P. pastoris‚ Pichia pastoris; VTS‚ vacuolar targeting signal; PCR‚ polymerase chain reaction; PAGE‚ polyacrylamide gel electrophoresis; SDS‚ sodium dodecyl sulfate Abstract A yam (Dioscorea opposita Thunb) class IV chitinase‚ whose genomic
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peroxidase‚ manganese peroxidase‚ and laccase‚ enzymes considered key for lignin degradation‚ providing a major advantage for its use in bioenergy research. • The draft genome will accelerate functional genomics research‚ helping to understand the molecular basis of lignin decay by this fungus as well as advancing its enzymatic applications. 1.Data The genus Trametes Fr. (Polyporaceae‚ Basidiomycota) comprises 20 species usually growing on decaying wood of angiosperms [1]. Trametes is morphologically
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EXAM QUESTIONS 1) Describe the alternative fates of pyruvate in cellular respiration. 2) Write notes on the structure and significance of α and β glycosidic bonds. 3) Describe the mechanism of DNA duplication. Include a brief account of the types of proteins‚ cofactors and enzymes involved. 4) Write an account of two of the key historical experiments that identified DNA as the carrier of the genetic code. 5) Describe the “central dogma” for the genetic code and the basis by which genetic information
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transduction paradigms and the biochemistry of hormones. The structure‚ function and replication of the cell’s genetic material. This information is reviewed in a series of lectures on DNA‚ the genetic code‚ protein synthesis and aspects of molecular biology. The digestion and absorption of biomolecules is reviewed and the consequences of malfunction considered. A number of disease states are used to illustrate selected principles including the relationship between nutrition and disease; atherosclerosis
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1 THE LURIA-‐DELBRÜCK EXPERIMENT The Luria-Delbrück Experiment Henry Paige University College Freiburg 2 THE LURIA-‐DELBRÜCK EXPERIMENT Abstract This paper discusses Luria and Delbrück’s 1943 Fluctuation Test. Historical background is first addressed to contextualize the experiment and define the theories‚ ideas‚ and discussions relevant at the time of the experiment. A brief summary of biologists’ knowledge and attitudes towards bacteria is explained through contemporary quotes. The two main
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lab is the identification of unknown bacteria and viruses in a lab. Selective vs. Differential Media Selective vs. Differential Media Use the following website to help you answer Q 1 and 2 http://www.highlands.edu/academics/divisions/scipe/biology/labs/rome/selectivedifferential.htm 1. What is a selective medium? What makes the medium selective? Name 2 examples (3 pts.) A selective medium is a medium that contains antimicrobials‚ dyes or alcohol that supports the growth of certain organisms
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