NA transformation of E. coli: The two plasmids were added to individual tubes containing E. coli and one with no plasmids. The three samples of E. coli were heated in a 42°C water bath for 90 seconds to heat shock the bacteria so that the plasmids would be taken up by the E. coli. These samples were then incubated at 30°C for half an hour and then plated on LB agar. Each tube was plated on an LB plate and a LB + ampicillin plate. Ampicillin is an antibiotic that is effective against E. coli‚ both
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The purpose of this experiment was understand the process of transformation and the effects on gene expression. The pGLO(-) culture had growth on the LB medium‚ while the LB amp and LB amp + ara mediums had no growth. It was expected that the LB medium had growth on the plate because it served as a control. The LB amp and LB amp + ara had no growth or glow under UV light because they were not successfully transformed and still contained the antibiotic ampicillin that prevented the growth of E. coli
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For the completion of this experiment the procedures were guided with the Rainbow Transformation1 lab manual. An Escherichia coli bacterial reference plate was used to obtain colonies which were resuspended into a CaCl2 solution that was previously kept on an ice bath. The rainbow transformation mixture containing the plasmid DNA was then added to half of the E. coli cells. These cells were later placed into a water bath set to 42ºC and “heat shocked” to promote the entrance of DNA into the cells
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The identification of Bamboo using various PCR and Sequencing Techniques Abstract Often the incorrect bamboo species is sold to unsuspecting customers at shops. This can have a disastrous effect on their garden. Three separate and unknown Bamboo leaf samples were taken and were required to be distinguished genetically from one another. Using ITS-PCR DNA amplification techniques‚ the ITS region DNA was amplified and used in PCR-RFLP and RAPD PCR in order to determine the genetic identity of each
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Karla Miramontes A. Giardino March‚ 22‚ 2015 Say No To GMO! Have you ever payed attention to the fruit sitting in your basket at home? How about an apple that you eat half of and then set it down because you’re full? Do they rot quickly or stay looking ripe and shiny for a decent amount of time? If you’re picturing your fruits ripe and shiny at all times‚ it has probably been tampered with. Science calls this Genetic Engineering. Genetically modified organisms ‚ also known as GMOS
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What is a genetically modified organism? A genetically modified organism (GMO) is an organism whose genetic material has been altered using techniques in genetics generally known as recombinant DNA technology. Viruses‚ bacteria‚ fungi‚ plants‚ and animals are all examples of organisms that have been engineered so that they contain genes from at least on unrelated organism. To create a genetically modified organism a molecule from a different source are combined into another molecule to create
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Experiment title: Extraction of Bacteria Plasmid DNA and Analysis of extracted DNA Samples Objectives: 1) To study and understand the steps for extract bacteria plasmid DNA. 2) To measure the concentration and purity of extracted DNA by using spectrometric method and agarose gel electrophoresis method. 3) Determine the size of extracted DNA by using agarose gel electrophoresis method. Materials and Methods: (Refer to UDEE2124 lab manual from page
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October 3‚ 2013 Bio Tech Essay Restriction enzymes are named according to the organism that they are kept in. The way that they create the name is to use the first letter of the genus‚ and after the first two letters of the species. There are only a certain amount of strains‚ sub-strains of a certain species that can produce restriction enzymes. A roman-numeral is always used to show one out of possible several different restriction enzymes created by the same organism or by different
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Cell Lab Report Experiments : 1. PCR . 2. Protein extraction and purification . 3. Protein concentration determination . 4. SDS-PAGE . 1. The aim of experiments : 2.1 The aim of PCR experiment is to replicate some DNA dimmers by using specific enzymes used for replication in vitro which is done in lab not by living organisms. 2.2 The aim of protein extraction and purification experiment is to extract some proteins and purify them by specific methods.
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Extract DNA from Anything Living |[pic] | |Introduction: [pic] Since DNA is the blueprint for life‚ everything living contains DNA. DNA isolation is one of the most basic and essential techniques in the study of DNA. The extraction of DNA from cells and its purification are of primary importance to the field of biotechnology and forensics. Extraction and purification of DNA are the first steps in the analysis and manipulation of DNA that allow scientists to detect genetic disorders‚ produce DNA
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