Abstract Human diseases such as Lyme disease (borreliosis)‚ Rocky Mountain spotted fever and Ehrlichiosis continually affect the population of Tamaulipan Biotic Providence‚ area shared between Texas and Mexico. The tick Ixodes scapularis tends to be the main carrier for Borrelia bacteria‚ which causes borreliosis. Nonetheless‚ its absence in the area studied gave cue to the analysis of another tick‚ Amblyomma inornatum. It is important to characterize the disease potential of the latter tick in
Premium Lyme disease Gel electrophoresis Polymerase chain reaction
Lactate dehydrogenase (LDH) is an enzyme involved in producing energy after the body has lost access to oxygen. LDH produces energy by helping to catalyze the reaction of NADH to NAD+ and does so by oxidation using pyruvate (1). LDH is found in highest concentrations in the heart‚ kidneys‚ lungs‚ blood cells and muscle tissues. Increases in LDH levels in the body have shown to be a marker of pain severity. This is because of tissue damage is the primary source of increasing LDH in the body. Thus
Premium DNA Gene Molecular biology
3.6.5. Southern hybridization Southern blot hybridization analysis was carried out using standard procedures (Sambrook et al. 2001). Approximately 20 µg of total genomic DNA from transgenic plants and untransformed control plant was digested with EcoRI‚ and then separated by electrophoresis on 0.8% agarose gel. Digestion profile was checked with 1µg DNA‚ with single cutter enzymes EcoRI (10U) in T-DNA region. For southern experiment‚ 20µg DNA was digested overnight. Restricted fragments were separated
Premium Molecular biology Western blot Southern blot
However relative quantification of gene expression using qRT-PCR is highly influenced by the expression stability of internal control or reference genes used for transcript normalization of target genes. The use of inappropriate or unstable reference gene(s) can seriously impact the transcript quantification results leading to false inferences or misinterpretations21‚22. Accurate normalization is thus necessary for obtaining biologically meaningful expression data and hence qRT-PCR analysis greatly
Premium Gene expression Molecular biology Gene
Lab Report Part II Purpose: To be familiarized with the science and techniques used to identify different types of bacteria based on their DNA sequences. Background Information: The process begins with preparing a sample. Successful identification starts with using a sample that is considered to be good. The first step is to pick up a single colony and drop it into a microcentrifuge tube. A buffer is used to dissolve the cell wall in order to extract bacterial DNA. This step may take several hours
Premium DNA Polymerase chain reaction DNA replication
Mujtaba Zafar 10/15/14 Michael Hadjiargyrou Bio 440 Answers only: Alignment statistics for match #1 Score Expect Identities Gaps Strand 385 bits(208) 2e-103 229/238(96%) 5/238(2%) Plus/Plus Query 1 AGTTATCATGGCCTCCCAAACCACTCACAAAGAAGAAAAAGAGTTTCCAGGAACTAGAAG 60 ||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||| Sbjct 2774 AGTTATCATGGCCTCCCAAACCACTCACAAAGAAG-AAAAGAGTTTCCAGGAACTAGAAG 2832 Query 61 ATGATTTAG
Premium DNA Gene RNA
ASU BIO 100 Exam 4 Practice Questions 1. Because of evolutionary descent‚ many species share characteristics with other species to which they are related. Indeed‚ according to evolutionary theory‚ all living species are descended from some common ancestor in the ancient past. What evidence supports this conclusion that ALL living species are related to each other? Shared traits in the fossil record; Genetic Code; DNA Structure of all living organisms 2. For some time‚ Russian prisons have been
Premium DNA Protein Enzyme
Cloning of plasmid pUC19 in E.coli bacteria Introduction One aspect of the DNA cloning experiments that is carefully considered is the selection of cloning vectors. A variety of vectors have been created‚ each being suitable for a particular use. One common vector used in laboratories is a plasmid called pUC19. It is 2686 base pairs long and possesses an origin of replication which allows the production of over 100 copies in a competent E.coli cell. It possesses a multiple cloning site (MCS)
Premium DNA Molecular biology
DNA Sequencing As of last few weeks‚ the transformation lab is performed to convey and purify a given protein. However after further research scientists found out that Transformation is not only used to purify protein but also to find out contents that are stored in a given plasmid. The objective of the lab that is to be performed involves a procedure that determines the identity of an unknown gene replicated in a plasmid. To begin this procedure two to four colonies of bacteria is added to two
Premium DNA Molecular biology Bacteria
Transfection for HMECs using Lipofectamine LTX + Plus reagent NB: • Using PLUS™ Reagent (Cat. No. 11514-015) enhances transfection performance in HUVEC cells. • The addition of antibiotics to media during transfection may result in cell death Transfection of HMECs Use this procedure to transfect plasmid DNA into HMECs cells in a 12-well format All amounts and volumes are given on a per well basis. 1. Cell density should be 50~80% confluent on the day of transfection (use the normal growth
Premium Bacteria Plasmid DNA