"Molecular biology" Essays and Research Papers

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    Light Blue Lab Report

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    Draw lines to represent where the bands will be. 6‚400 5140 4600 4310 4240 4160 3820 2880 2520 2500 2000 *light blue is 1 band Experiment The group first designed the materials to create and hold the .75 agarose gel and experiment. Everything except the comb was made of ¼ inch Acrylic Plexiglass. The glass helped the group see what was going on in the experiment. The comb was 3D printed to be more accurate. One material was a casting tray which held the Gel in future steps.

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    Noggin And Rna Case Study

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    Quiz De Almeida et al. Calreticulin is a secreted BMP antagonist‚ expressed in Hensen’s node during neural induction 1) Explain two lines of evidence that Noggin‚ Chordin‚ and Follistatin are not the only neural inducing factors during development. Targeted mouse deletion in Noggin‚ Chordin‚ or Follistatin genes on their own has only minor effects on neural development. Elimination of Noggin and Chordin produces a headless phenotype but neural tissue still forms‚ meaning deletion of these genes

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    Genetic Synthesis

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    The main goal of this experiment was to successfully clone human insulin cDNA into an expression vector. The gel electrophoresis results confirm that the inserts were successfully cloned into the expression vector based on the identical fragment position of each cut candidate in the gel. The fragment sizes of the both cut candidates produced on the gel when compared to the DNA ladder were larger‚ at roughly about 6000 base pairs and 6800 base pairs‚ than the predicted size of 2315 and 3119 base pairs

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    Lab Report (Scientific Paper) 2: Bacterial Transformation;DNA Extraction Part I & II:Total Genomic Extraction & Plasmid Extraction;Electrophoresis By:Chris Foster Abstract: We conducted three experiments that included a Bacterial Transformation‚ a two process DNA extraction‚ and a final procedure using gel electrophoresis. The Bacterial Transformation lab was performed to prepare the plasmid into a bacteria and to use that bacteria to amplify the plasmid in order to make large quantities

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    CRISPR Ethical Issues

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    CRISPR is a new technology‚ a method that can edit genomes. It makes it possible for scientists to manipulate and make changes to the DNA in the cells of all organisms. Since this is such a new discovery‚ there hasn’t been done much testing and experimenting‚ which makes it unknown and none predictable for humans. It is said that with CRISPR‚ scientists will for example‚ be able to change genes in human embryos‚ and create so called “designer-babies”. There has been a lot of discussion around this

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    To compare the Shannon Diversity index between the Riparian and Coastal Sage Scrub community‚ acquiring the actual value of the Shannon Diversity index from both of these communities needs to be done first. This can be done through the assistance of these materials: a 50m Fiberglass Metric Long Tape Measure with a Pumpkin color‚ GPS Tracker‚ lab notebook with a reasonable amount of paper‚ black-inked pen‚ and a functioning calculator. First‚ in either the Riparian or Coastal Sage Scrub community

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    1. Before sampling began‚ observations were made while examining each population’s distribution pattern. The red-capped introverts have a uniform distribution due to their even spacing between organisms. The blue-footed wanderers have a random distribution as a result of their unpredictable spacing. Lastly‚ the green-chested bar-hoppers have a clumped distribution as they were together in groups or “clumps”. There are other ways to determine which population distribution pattern a population

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    Strawberries Investigation

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    AbstractThis experiment investigated the amount of DNA extracted from strawberries. This was done by using the independent variable of alcohol to affect the dependant variable of the amount of DNA extracted. This was done to find out if primary or secondary alcohol would create more DNA precipitate than the other. For this the primary alcohols used were; methanol and ethanol‚ and the secondary alcohol was; isopropyl. Of this the secondary alcohol‚ isopropyl was discovered to be the most effect alcohol

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    Dna Isolation Lab Report

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    PLASMID DNA ISOLATION‚ RESTRICTION ENZUME DISGESTION AND AGAROSE GEL ELECTRIPHORESIS Abstract: The gel is covered with an ion- containing buffer‚ such as (TAE)‚ that controls the pH of the system and conducts electricity. overall DNA concentration was lower than expected. Using agarose gel electrophoresis is to separate and visualize the DNA fragment‚ which is produced by restriction enzymes . Introduction: The purpose of this experiment is to measure the size of the fragments of DNA and separate

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    In this laboratory‚ two experiments were performed; the first one was performed to determine whose fingerprint was found at a crime scene. For this experiment‚ four-10 mL DNA samples were placed in a well of agarose gel‚ using a micropipette; the sample was also covered in a TBE buffer solution. The four samples were then put in the electrophoresis apparatus to be run through electrophoresis. After 20 minutes‚ the samples were ready and the results were documented; it was found that suspect “B”

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