2/15/2013 background on transformation of bacteria with pGLO plasmid Experiment #5 Aim: Purpose of this lab is to have plasmid activity transformed Material: Bacteria starter plate‚ pGLO DNA Plasmid‚ microcentrifuge tubes‚ Ice‚ water bath‚ CaCl2 Transformation solution‚ (LB) agar plate‚ (LB/Amp) agar plate‚ (LB/Amp/ara) agar plate‚ Micropipette‚ and Micropipette tips. Method: Genetic transformation is a procedure which is done by taking genes from one organism and putting them in another organism
Premium Plasmid Gene DNA
Gattaca Assignment In the movie Gattaca‚ three social laws evident throughout the film that relate to present rules in society today include DNA discrimination‚ the assortment of valids and invalids‚ and the exclusion of the disabled. The technique and relation of these futuristic laws verify that just because genetic manipulation is possible‚ does not mean that it should be used in transforming and modifying offspring. In the movie‚ DNA discrimination is apparent from the beginning. The idea
Premium Discrimination DNA Law
In 1989‚ Altman and Cech won a Nobel Prize in Chemistry for their discovery of catalytic RNA – which was instrumental for the RNA World Hypothesis ("Sidney Altman: The RNA World"). This hypothesis stated that early life started with RNA as they possess the ability to serve as a catalyst as well as contain genetic information. The RNA was able to spontaneously form under natural processes‚ which eventually replicated into all sorts of life. This paper will analyze the theoretical and experimental
Premium DNA Gene Genetics
II-Leukocidin genetic organization and genome distribution The genetic architecture of the S. aureus leucocidins is widely conserved. F and S subunits of a certain toxin pair are transcribed from specific single promoter. The open reading frame of the S subunit immediately come before The open reading frameof the F subunit‚ with as little as a single nucleotide separating each open reading frames (Fig.1). The only known exception to this rule iwas in gamma-hemolysin locus. 1-Gamma-hemolysin:
Premium DNA Gene Protein
Software fusion is the combination of separate sets of CT and PET images into one study unit. A number of commercial packages have been established to suit this purpose. The packages normally have similar functions sets for visualization and fusion. The most common image fusion technique is manual setting of a single scan relative to another positioned in a 3- dimensional space‚ comprising of 9 free position adjustment parameters‚ each of them rotation in 3 directions and also size. This
Premium DNA Gene Genetics
2. Material and methods 2.1 Sample collection The following 14 samples of Staphylococcus aureus were used in this study. All samples were obtained from food contact surfaces at three food premises in University of Putra Malaysia (UPM). Samples were collected from the surface of each material according to standard swabbing techniques. The collection was carried out during weekdays (15:00-17:00) after finished the preparation of foods. Sterile swabs were removed from the coded test tubes that contain
Premium DNA Gene Protein
Formaldehyde as a model protein crosslinking reagent. Understanding protein-protein interactions is becoming a vital study for application in the development of new age pharmaceuticals and utilization in diagnostic models. The overall aim is to gain an insight into the function and role of individual proteins with specific interest on how they react in cells‚ (Toews et al. 2008). The biopharma industry in Ireland is becoming a booming success for the economy therefore a complete understanding of
Premium DNA Gene Protein
2.6. In vitro characterization of prepared cubogels 2.6.1. Physical evaluation of the prepared cubogels The freshly prepared cubo-gels were examined by visual inspection‚ where the prepared formulae were examined for their physical characteristics (e.g.‚ color and homogeneity). In addition‚ the pH of a 10 % aqueous solution of the Cl-cubogels was measured. 2.6.2. Rheological properties evaluation of the prepared Cl-cubogels The prepared Cl-cubogels were evaluated a rotational Brookfield viscometer
Premium Protein Chemistry Water
What is a gene? A gene is a continuous DNA segment that is transcribed into a single preRNA molecule. A zoologist and geneticist named Thomas Hunt Morgan is known for his research with fruit fly. Thomas Morgan was born on September 15‚1866 in Lexington‚ Kentucky. In 1886‚ he received his B.S. degree at the University of Kentucky. In 1910‚ Morgan saw that one male fly had a distinct difference about it. Instead of normal red eyes‚ this fly had white eyes (Allen). He wanted to test the breeding of
Premium Gene Genetics DNA
The purpose of this experiment was to determine if there is a difference in invertebrate diversity between the mainland and Cow Island at the Queen’s University Biological Station. This was done by setting up multiple transect lines in both locations‚ all running 60 metres long with sticky traps and pitfall traps every 15 metres. The point-quarter transect method was used to record surrounding tree species and diameter to provide further information about the areas biotic diversity. It was hypothesized
Premium DNA Gene Bacteria