11M01_ZAO2177_01_SE_CH01.QXD 3/4/11 1:36 AM Page 1 E X E R C I S E 1 Cell Transport Mechanisms and Permeability P R E - L A B Q U I Z 1. Circle the correct term. A passive process‚ diffusion / osmosis is the movement of solute molecules from an area of greater concentration to an area of lesser concentration. 2. A solution surrounding a cell is hypertonic if: a. it contains fewer nonpenetrating solute particles than the interior of the cell. b. it contains
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Objectives: a. to define diffusion and demonstrate this process in gases b. to cite molecular weight and time as two factors affecting the rate of diffusion c. to formulate a hypothesis on the relationship of each of these factors on the rate of diffusion d. to conduct and experiment to determine the effects of the two factors on the rate of diffusion e. to compute the partial rate and average rate of diffusion f. to conclude on the relationships of molecular weight and time on
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Introduction A sample of DNA found in a crime scene was provided along with five suspects. Their DNA was then processed using restriction enzymes and Agarose Gel Electrophoresis. The objective of this lab was to match a criminals DNA to a crime scene using restriction enzymes EcoRI and Pstl with Agarose gel electrophoresis. Restriction enzymes cut DNA at a specific base pair site recognized by the enzyme‚ which then turns one single strand of DNA into many fragmented strands of DNA. EcoRI recognizes
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Simple Diffusion 1. The following refer to Activity 1: Simulating Dialysis (Simple Diffusion). Which solute(s) were able to pass through the 20 MWCO membrane? According to your results‚ which solute had the highest molecular weight? ______________________________________ Which solute displayed the highest rate of diffusion through the 200 MWCO membrane? __________________________ Using the data from Chart 1‚ explain the relationship between the rate of diffusion and the size of the solute.
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Simple Diffusion 1. The following refer to Activity 1: Simulating Dialysis (Simple Diffusion). Which solute(s) were able to pass through the 20 MWCO membrane? None of the solutes were able to pass through the 20 MWCO membrane. A possible reason to why none of the of these solutes were able to pass through was because the solutes could have been too big to be transported. According to your results‚ which solute had the highest molecular weight? The solute that had the highest molecular weight
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Review Sheet 1 Results 1. Describe two variables that affect the rate of diffusion. Your answer: Two variables that affect the rate of diffusion are the MWCO membrane and the solute concentration. Increasing the membrane size and solute concentration will also increase the average diffusion rate. Decreasing the membrane size and solute concentration will reduce diffusion rates and can even prevent all diffusion. 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane
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experiments were conducted. These tests were done over simple diffusion‚ facilitated diffusion‚ osmosis‚ filtration‚ and active transport by changing and observing different variables and how they affect transport through the membrane. Obtained was the understanding of the changing of the molecular weight cut off in a membrane‚ and how it does not play a part directly in changing the rate of diffusion‚ but instead determined if diffusion was accomplishable at all. Knowledge on the size of molecules
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Introduction: Membrane Transport • What is membrane transport • Types – – – – Diffusion Osmosis Active Transport Endocytosis/Exocytosis Photo Courtesy Of: http://hyperphysics.phy-astr.gsu.edu/hbase/biology/celmem.html Osmosis • Example: A semipermeable membrane bag containing a 30% sugar solution is placed in a beaker of pure water. – Diffusion or osmosis? – What should we expect to see? • Direction of molecular movement Photo Courtesy Of: https://internationalgcsebiology.wikispaces.com/Movement+in+and+out+of+cells
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Lab 1: Introduction to Science Exercise 1: Data Interpretation Table 4: Water Quality vs. Fish Population Dissolved Oxygen (ppm) 0 2 4 6 8 10 12 14 16 18 Number of Fish Observed 0 1 3 10 12 13 15 10 12 13 Questions 1. What patterns do you observe based on the information in Table 4? 2. Develop a hypothesis relating to the amount of dissolved oxygen measured in the water sample and the number of fish observed in the body of water. 3. What would your experimental approach be to
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Activity 1: Simulating Dialysis (Simple Diffusion) Lab Report Pre-lab Quiz Results You have not completed the Pre-lab Quiz. 09/20/11 page 1 Experiment Results Predict Question: Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane? Your answer : c. No‚ not at all. Predict Question 2: Recall that glucose is a monosaccharide‚ albumin is a protein with 607 amino acids‚ and the average molecular weight of a single amino acid is 135
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