Introduction: Escherichia coli (E.coli) is a commensal-pathogenic organism which include a wide range of strains. There are several advanced molecular-genomic technologies that are capable for detecting and identifying different strains of E.coli. But‚ ERIC-PCR technique is a quick and cost effective method for determining individual strains via demonstration of strain specific fingerprint bands. Therefore‚ the ERIC-PCR technique was used to determine the isolated strains of E.coli from different
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preparation. The molecular weight of the fibrinolytic protease was determined by comparison of the migration distances of standard marker proteins (Lysozyme 14.4 kDa‚ Soybean Trypsin Inhibitor 21.5 kDa‚ Carbonic anhydrase 31.0 kDa‚ Ovalbumin 45.0 kDa‚ Bovine Serum Albumin 66.2 kDa and Phosphorylase b 97.4 kDa). Depending on the relative mobility‚ the molecular weight of the protein band was calculated to be around 31 kDa. Thus‚ it was concluded that our fibrinolytic protease enzyme has a molecular weight of
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converted to a percentage grade when registered in your “Gradebook”. |ACTIVITY 1: Simulating Dialysis (Simple Diffusion) |Answers | |The molecular weight of urea is approximately 60. Which of the membranes can it pass through? |D | |50 MWCO membrane
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BIODEL INC. EXECUTIVE SUMMARY Biodel’s use of biotechnology expertise in the three prime areas of Cell Biology‚ Molecular Biology‚ and Immunodiagnostics has led their company into some great opportunities. Biodel created a synthetic serum to replace fetal calf serum and horse serum‚ and provide a more consistently available product for scientific researchers. Biodel’s Molecular Biology division used DNA technology to gain 60% of the market share of growth factors and stock the largest collection
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EXPERIMENT NO. 15 PROTEIN CHARACTERIZATION BY ELECTROPHORESIS Abstract The molecular weights of protein extracts were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two sets of four protein samples‚ standard bovine serum albumin (BSA)‚ invertase‚ egg albumin‚ and casein‚ were prepared; one set containing β-mercaptoethanol (BME) while the other did not. These were then analyzed through SDS-PAGE with 12.5% resolving gel‚ prepared using 2 M Tris-HCl at pH 8
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The effect of the molecular weight to the rate of diffusion Based on the experiments‚ sd f f f f s wrefwe fwe rfwjv rwefhnw fwer fwe fwef fwe fwe fwe fwef wef w fwe fwe From Wikipedia‚ the free encyclopedia Jump to: navigation‚ search Sucrose is the organic compound commonly known as table sugar and sometimes called saccharose. This white‚ odorless‚ crystalline powder has a pleasing‚ sweet taste. It is best known for its role in human nutrition. The molecule is a disaccharide derived from glucose
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said‚ "I need a caretaker” So God made a farmer” (Harvey). Farming gets a bad rap for a lot of things. Most of what people think about farming that do not have anything to do directly with it are misconceptions. Many people are up in arms about the rights and wrongs of factory farming. Tons of questions get brought up when people hear the term factory farming. The most famous question asks about the ethics of factory farming. Factory farming is ethical for three major reasons. It shows more efficiency
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Molecular Geometry A. Natural Orientation of Volumes about a Central Point. You will need 20 round balloons for this experiment. Join them together as indicated in the Balloon Arrangement column and then describe the shape in the space provided. Balloon Arrangement Description of the Shape Two-Balloon Set Linear Three-Balloon Set Trigonal Planar Four-Balloon Set Tetrahedral Five-Balloon Set Trigonal Bipyramidal Six-Balloon Set Octahedral B. Valence Shell Pairs: Single
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of carbon dioxide molecules = 6.022 × 1023 molecules 1 mole of sodium chloride = 6.022 × 1023 formula units of sodium chloride Molar mass of a substance can be defined as: Mass of one mole of a substance in grams Numerically equal to atomic/molecular/formula mass in u. Example − Molar mass of CO2 = 44.011 g mol−1 Molar mass of NaCl = 58.5 g mol−1 Examples 1. What number of moles contains 3.011 × 1023 molecules of glucose? Solution: 1 mole of glucose is equivalent to 6.022 × 1023 molecules
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Farming on a Whole New Level Although people have worked in agriculture for more than 10‚000 years‚ advances in technology assist with maintaining and protecting land‚ crops‚ and animal. The demand to keep food prices affordable encourage those working in the agriculture industry to operate as efficiently as possible (Newman and Ruiz 33-47). Almost all people and companies in this industry have many acres of land they must maintains‚ and it is not always feasible for farmers to take frequent
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