Farming Methods Eng. /101 How was your meat or veggies produced? Wouldn’t you like to know how and what the farmers use or how they grow the food that you will be eating? It is always good to know how you food to prepared before you decide to put it into your body. Just think if you were going to buy a car and you didn’t find out if it was wrecked before or how it was maintained and then you get it home and it breaks down. This essay will help you understand the different types of
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info in figures and tables). Lecture 1 Processes that utilize recombinant DNA technology (and molecular processes that don’t) Basic science; what it is‚ how it is performed and why it is important in biotechnology Bioinformatics defined Be able to name some non-life science disciplines important in use of biotechnology Compare and contrast alcohol and lactate (lactic acid) fermentation Molecular Pharming defined Animal Biotech for producing products and for research Bioremediation basics
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How well did the results compare with your prediction? Urea molecules are too large (molecular weight of Urea is 60.07 g/mol) to pass through the 20 MWCO membrane. The results of the experiment correlated with my prediction. 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? As expected‚ glucose‚ with a molecular weight of approximately 180 g/mol‚ readily diffused across the 200 MWCO membrane;
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Simple Diffusion Activity 1: Simulating Simple diffusion 1. What is the molecular weight of Na+? 22.99 2. What is the molecular weight of Cl-? 35.45 3. Which MWCO dialysis membranes allowed both of these ions through? 50‚ 100‚ and 200 4. Which materials diffused from the left beaker to the right beaker? NaCl‚ Urea‚ and Glucose at MWCO 200 5. Which did not? Why? Albumin‚ too large to diffuse Activity 2: Simulating Dialysis 1. What happens to the urea concentration in the left beaker
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Chapter 4 Carbon and the Molecular Diversity of Life Lecture Outline Overview: Carbon – The Backbone of Biological Molecules * Although cells are 70–95% water‚ the rest consists mostly of carbon-based compounds. * Carbon is unparalleled in its ability to form large‚ complex‚ and diverse molecules. * Carbon accounts for the diversity of biological molecules and has made possible the great diversity of living things. * Proteins‚ DNA‚ carbohydrates‚ and other molecules that
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WESTERN BLOTTING (Written Report) I. PRINCIPLE Western blotting is a method for identifying a specific protein in a complex mixture and simultaneously determining its molecular weight. In Western blotting‚ proteins are electrophoresed into a gel‚ as the proteins migrate through the gel they are separated based upon size and charge. Characteristically‚ smaller proteins migrate through the gel faster than larger proteins. Sufficiently separated proteins in an SDS-PAGE can be transferred to a
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gave ions to support the conductivity for the gel to run. The uncut λ DNA would be added to labeled tube as a control DNA. By comparing the conformations of uncut DNA with the cut DNA‚ the completed digestion could be verified. Molecular marker‚ which was used for molecular weight calculation‚ was stored in another sample tube. The prepared sample contained puC18 DNA would be run as the degrade DNA control
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Lab Report One Exercise Five Activity One “Simulating Simple Diffusion” Activity Two “Simulating Facilitated Diffusion” Jessica Ogola Anatomy and Physiology 2401 Dr. Denyse Jones 02/02/2012 Objective: The objective of this experiment is to perform the simulation of the movement of solutes from a higher concentration to a lower concentration within a given amount of time. The goal of the exercise is to provide a simulation for the process of the facilitated diffusion of
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Gel Filtration Gel filtration is a non-adsorptive chromatography technique that separates molecules on the basis of molecular size. Desalting and buffer exchange are two special examples of gel filtration that are widely used in many downstream bioprocesses. Desalting is used to completely remove or lower the concentration of salt or other low molecular weight components in the sample while buffer exchange replaces the sample buffer with a new buffer. Gel filtration is one of the easiest chromatography
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concentration values explain the bands thickness and color. The smear lastly is Gamma globulin although it has a close concentration as alpha 1 and beta globulin it has smeared zone and that’s due to its light molecular weight‚ so we conclude that another reason for the band thickness is the molecular weight of the
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