crystal (Methylene blue‚ solid or Potassium Permanganate KMnO4-purple) will move further than the other due to the driving force (kinetic energy)? My prediction is that Potassium Permanganate KMnO4- purple (molecular mass- 158.03 g/mol) will move further than Methylene blue‚ solid (molecular mass- 319.85 g/mol) since it weighs less. In osmosis is diffusion of H2O across a selectively permeable membrane (dialysis tubing). There is only movement of water‚ not solute‚ across the plasma membrane. Water
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Characterization of a Yam Class IV Chitinase Produced by the Recombinant Pichia pastoris X-33 Department of Botany‚ Jahangirnagar University‚ Savar‚ Dhaka 1342‚ Bangladesh Abbreviations: Endo H‚ Endoglycosidase H; PR protein‚ pathogenesis related protein; P. pastoris‚ Pichia pastoris; VTS‚ vacuolar targeting signal; PCR‚ polymerase chain reaction; PAGE‚ polyacrylamide gel electrophoresis; SDS‚ sodium dodecyl sulfate Abstract A yam (Dioscorea opposita Thunb) class IV chitinase‚ whose genomic
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References: M. F. Rubner‚ Molecular Electronics‚ Research Studies Press‚ 1992‚ chapter 2 Luis Alcacer‚ Conducting Polymers‚ D. Reidel Publishing Company‚ 1987 Herbert Naarmann‚ Polymers to the Year 200 and Beyond‚ John Wiley & Sons‚ 1993‚ Chapter 4 Ian M. ‚ Introduction to Synthetic
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Expressing and Purifying the Recombinant form of Green Fluorescent Protein (rGFP) from the E.coli strain using Ni2+ agarose affinity chromatography technology Abstract The purpose of this experiment was to express and purify the his6-tagged recombinant form of GFP (rGFP) from the organism E.coli using Ni2+ agarose affinity chromatography. The expression of rGFP was confirmed qualitatively using the UV light and was expressed in the E.coli strain BL21 (DE3) (-- removed HTML --) (-- removed HTML
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vertical glass tube over the top of which a stream of vapor-free gas is passed. A water bath is provided for maintaining a steady temperature so that there is no eddy current in the vertical tube and mass transfer takes place from the surface by molecular diffusion alone. The rate of evaporation can be followed by the rate of fall of the liquid surface. A traveling microscope is provided for determining‚ the liquid fall. With the knowledge of the concentration gradient‚ the diffusivity of the vapor
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The purpose of this study is to produce recombinant DNA molecules to produce bacteria that would transform into red fluorescent proteins. One plasmid was that allowed to express a red fluorescence was produced by recombining two plasmids by using molecular techniques. Agar plates labeled LB‚ LB/AMP‚ and LB/AMP/ARA containing ampicillin (AMP) and arabinose (ARA) were used to grow of the bacteria of interest and SDS-PAGE gels were utilized in identifying the fluorescent and non-fluorescent proteins
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http://www.mhhe.com/physsci/chemistry/essentialchemistry/flash/hybrv18.swf Salient Features Chemistry animation and videos Atomic orbital animations Hybridization animation http://jchemed.chem.wisc.edu/JCEDLib/WebWare/collection/open/JCEWWOR019/mo Molecular orbital theory animations movies.html http://www.mhhe.com/physsci/chemistry/essentialchemistry/flash/activa2.swf http://dwb4.unl.edu/chemAnime/Electro.htm Activation energy animation Electrochemistry animations RW-7 RW-8 RW-9 http://www.mhhe
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EXAM QUESTIONS 1) Describe the alternative fates of pyruvate in cellular respiration. 2) Write notes on the structure and significance of α and β glycosidic bonds. 3) Describe the mechanism of DNA duplication. Include a brief account of the types of proteins‚ cofactors and enzymes involved. 4) Write an account of two of the key historical experiments that identified DNA as the carrier of the genetic code. 5) Describe the “central dogma” for the genetic code and the basis by which genetic information
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Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss‚ Inc. ISBNs: 0-471-37972-7 (Paper); 0-471-22390-5 (Electronic) 12 Electrophoresis Martha L. Booz Chemical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . What Is the Safest Approach to Working with Acrylamide? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . What Are the Symptoms of Acrylamide Poisoning? . . . . . . What
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Make Recombinant DNA? Recombinant DNA Technology May Allow Us To: • Cure or treat disease • Genetically modify our foods to increase flavor‚ yield‚ nutritional value or shelf-life • Better understand human genetics • Clone cells or organs Molecular Biology’s Best Friends: Bacteria Why use bacteria? • They’re relatively simple organisms. • They reproduce very quickly and asexually (this means that the “daughter” cells will contain the exact same DNA as the “parent” cell). • It’s pretty easy
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