band‚ spacing pattern of bands) you collected for the samples (fish‚ crab‚ food‚ protein ladder) in the appropriate columns. The approximate molecular weight of a band is determined by comparing the band of the food sample to the protein ladder bands. Sample Fish (1) Crab (2) Food (3) Protein Ladder (M) Number of Bands 5 2 2 6 Approximate Molecular Weight of the Bands Spacing Pattern of Bands 1 separated from 2 pair of bands spaced apart 2 bands at 45 and 29 kd 2 bands at 45 and
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BIOLOGY 1201‚ Section #2 Spring‚ 2014 (MWF 9:30-10:20 AM) Instructor: Dr. Terry M. Bricker Office: A606 Life Science Annex (Laboratory‚ A623 – check the lab if I am not in the office!) Phone: 225-578-1555 E-mail: btbric@lsu.edu -- E-mail is an excellent way to contact me or ask questions. Office Hours: M‚Tu 3:30-4:30‚ or by appointment. - Please see me if you have questions or problems. COURSE: BIOL 1201‚ General Biology‚ 3 credit hours. Biology 1201 is a course intended for students that are majoring
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Restriction Fragment Length Polymorphism and Southern Blotting 1. Abstract The aim of the experiment was to be introduced to the techniques involved in the identification of restriction fragment length polymorphisms. Restrictions were carried out using three different restriction enzymes‚ ECORI‚ HindIII and BstELI with their buffers. Lambda (λ) DNA was then examined using electrophoresis and Southern blotting. The results showed that λ DNA was best digested by EcoRI as all of the expected bands
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Nanotechnology is the engineering of functional systems at the molecular scale. This covers both current and work and concepts that are more advanced. Nanotechnology is the projected ability to construct items from the bottom up‚ using techniques and tools being developed to make complete‚ high performance products. Basically‚ nanotechnology is a field of research concerned with building things (materials and devices). What it does: increases efficiency of energy consumption‚ helps clean the environment
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Dr. Randy Schekman graduated from the university of California‚ LA in 1971 for molecular biology and got his Ph.D. in biochemistry at Stanford University in 1975. Now he is currently working at the university of California as a professor of cell and development biology and also works at the Howard Huges Medical Institute Investigator. His main focus for his research is in the molecular description of the process of membrane assembly and vestibular movement in the eukaryotic cells. His interest
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Simple Diffusion Activity 1: Simulating Simple diffusion 1. What is the molecular weight of Na+? 22.99 or 23 2. What is the molecular weight of Cl-? 35.45 3. Which MWCO dialysis membranes allowed both of these ions through?50‚100‚ 200 4. Which materials diffused from the left beaker to the right beaker? Urea‚ NaCl and glucose diffused 5. Which did not? Why? Albumin was too large to diffuse into the right beaker. Activity 2: Simulating Dialysis 6. What happens to the urea concentration
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1. SDS-PAGE of purified protein X. Lane 1‚ Protein ladder (in Daltons). Lane 2‚ purified protein X (affinity chromatography). Lane 3‚ purified protein X (company manufactured). Lane 4‚ elution buffer. a. What is the benefit of a protein ladder/molecular weight marker in an SDS-PAGE gel? Describe what you can learn about the protein bands found in lanes 2‚ 3 and 4 based on the protein ladder bands. b. Positive and negative controls are absolutely essential to the validity of experimental data. In
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Title: Diffusion throughout the membranes Lab Partner(s): Alexis Clouting Date: 2/15/15 Abstract: In the content of the Module 2 we learned about Diffusion across cell membranes. We touched on the different types of cells and their functions. How things are transported in and out of cells. Learning about isotonic‚ hypertonic and even hypotonic solution. This is not my first time touching on this subject in my nursing career and I learned a way to remember what happens in the different solutions
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(Appendix A). 1mL of the dye solution was added to 20uL of the protein sample‚ mixed and incubated for 10 minutes at room temperature before measuring the absorbance at 595nm using the spectrophotometer. 3.6 Verification for the Homogeneity and Molecular Weight of the DNA Polymerase
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think the urea was not able to diffuse through 20 MWCO? How well did the results compare with your predictions? The urea was not able to diffuse through the 20 MWCO because the membrane pores are too small and the urea molecules are large. The molecular weight of Urea is 60.07 g/mol which is too large thus the molecules were not able to pass through the pores of the 20 MWCO. The results that I obtained from the experiment agreed with these predictions because urea was no able to diffuse through
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