IPTG induction‚ salting out‚ thermostable polymerase Background: Taq polymerase is a thermostable enzyme essential in the PCR reaction that is isolated from recombinant E. coli. Results: Taq polymerase was isolated‚ the sample was not pure Taq‚ molecular weight found to be 114kDa. Conclusion: The methods isolated taq but an improvement of cation exchange and increase heat denaturation should be added. Significance: An improved method of Taq isolation offers a valuable resource for PCR. SUMMARY The
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Major Uses of Radioisotopes Americum-241 Used in many smoke detectors for homes and businesses…to measure levels of toxic lead in dried paint samples…to ensure uniform thickness in rolling processes like steel and paper production…and to help determine where oil wells should be drilled. Used to analyze metal alloys for checking stock‚ scrap sorting Cadmium-109 Calcium-47 Californium-252 Carbon-14 Cesuim-137 Chromium-51 Important aid to biomedical researchers studying
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By RF was finding the molecular weight of the enzyme. The molecular weight of 6-PGD is 44 KDa. Studying properties (characterization) of the enzyme while the enzyme is more active in optimum temperature at 60°C‚ in Tris-HCl PH=8. 0 shown in the figure (4.4.) high activity. The optimum ionic strength
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vector was used to clone the individual PCR products which then transformed into E. coli. Catalytically active domains were studied by analyzing deduced amino acid sequences of genes. SDS-PAGE analysis was carried out to estimate the molecular weights of proteins. Molecular weights of peh‚ celB and celC products were found to be 41.5 kDa‚ 29.5 kDa and 40 kDa‚ respectively. Agar diffusion assays were performed to qualitatively determine the activities of polygalacturonase and cellulase of the cloned
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Introduction: With the execution of this experiment‚ we began to go deeper into the Cell and Molecular Biology course. The main focus of the experiment would be how the Restriction Endonucleases cleave the strands of DNA. For this experiment‚ pBR322 was the specimen to use. Restriction Endonucleases work by cleaving the sugar phosphate backbone of specific DNA sites. Restriction enzymes that have been isolated from bacteria have a defensive role. This idea is illustrated when an attacking foreign
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peroxidase‚ manganese peroxidase‚ and laccase‚ enzymes considered key for lignin degradation‚ providing a major advantage for its use in bioenergy research. • The draft genome will accelerate functional genomics research‚ helping to understand the molecular basis of lignin decay by this fungus as well as advancing its enzymatic applications. 1.Data The genus Trametes Fr. (Polyporaceae‚ Basidiomycota) comprises 20 species usually growing on decaying wood of angiosperms [1]. Trametes is morphologically
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Plant Molecular Biology Reporter 23: 67–73‚ March 2005 © 2005 International Society for Plant Molecular Biology. Printed in Canada. Protocols An Improved Agrobacterium-Mediated Transformation Protocol for Recalcitrant Elite Indica Rice Cultivars K.K. KUMAR‚ S. MARUTHASALAM‚ M. LOGANATHAN‚ D. SUDHAKAR and P. BALASUBRAMANIAN* Rice Transformation Laboratory‚ Department of Plant Molecular Biology and Biotechnology‚ Center for Plant Molecular Biology‚ Tamil Nadu Agricultural University‚ Coimbatore-641003
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1 Introduction “ I think fur look much better on the animal than on the models” Stella Mc Cartney 1.1 Fur farming Animals have been kept in captivity for the sake of their fur since the end of the 19-century. In Norway however‚ this tradition is rather young‚ and the fur farming was not established until in 1920-30. [1] 31 million animals are raised and killed on fur farms each year. Mink account for 26 million‚ fox 4.1 million. Chinchillas‚ racoon dogs (not to be confused with the North American
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experiments are similar‚ but instead of trying to find a disease or infection in this study‚ actin and myosin was the focus. The purpose of this study was to examine if actin and myosin were present in different types of fish proteins by observing the molecular weights of the proteins using kaleidoscope‚ by western blotting. We examined Rainbow Trout‚ Catfish‚ Pollock‚ Salmon‚ Tilapia‚ Mahi-mahi‚ Cod‚ Lake Erie Perch‚ KD and Actin/Myosin Standard proteins and analyzed where the bands of the proteins were
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Do you know about the food that you are eating or how it is being grown? Genetically modified foods is the way scientists can make food crops stay fresher‚ grow bigger‚ and have the crops create their own pesticide. Sounds good right? Wrong! Genetically modified foods need to be monitored and removed from everyday agriculture because of the threat they pose to human health‚ the environment‚ and the impact on the global economy. Organic food can be a hazard to human’s health. There are three
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