concentration gradient. The molecular size vs the MWCO size of the membrane can either increase‚ decrease‚ or prevent diffusion. The greater the concentration gradient the greater the diffusion rate due to molecules moving from areas of higher concentration to areas of lower concentration. • Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? My prediction was correct. The molecular weight of urea is 60.07 and
Free Diffusion Molecular diffusion Osmosis
Christopher Lothman Geometry Distance Formula: Translation A’ (10‚ 1) B’ (2‚ 1) C’ (2‚ 7) Rule: (x‚ y) > (x 8‚ y + 4) A2C2 = sqrt(((2 (10))^2) + ((7 1)^2)) = 10 AC = sqrt(((6 (2))^2) + ((3 (3))^2)) = 10 A2C2 = AC A2B2 = sqrt(((2 (10))^2) + ((1 1)^2)) = 8 AB = sqrt(((6 (2))^2) + ((3 (3))^2)) = 8 A2B2 = AB B2C2 = sqrt(((2 (2))^2) + ((7 1)^2)) = 6 BC = sqrt(((6 6)^2) + ((3 (3))^2)) = 6 B2C2 = BC Triangle A2B2C2 = Triangle ABC by construction
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analyze and identify DNA by use of a spectro-photometer * to use restriction enzymes to cleave DNA into fragments * to visualize the restriction fragments by gel electrophoresis * to compare the different DNA fragments generated by use of molecular markers Abstract This work describes a lysis method for the isolation and purification of bacterial genomic DNA and visualization of the restriction fragments by agarose gel electrophoresis. It was noted that for one to isolate and purify bacterial
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ROLE OF BACTERIOPHAGES IN GENETICS AND MOLECULAR BIOLOGY INTRODUCTION AND DISCOVERY: A bacteriophage is any one of a number of viruses that infect bacteria. Bacteriophages are among the most common biological entities on Earth. The term is commonly used in its shortened form‚ phage.Typically; bacteriophages consist of an outer protein capsid‚ enclosing genetic material. The genetic material can be ssRNA‚ dsRNA‚ ssDNA‚ or dsDNA along with either circular or linear arrangement. Bacteriophages are
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through the 20 MWCO because the pores of the membrane were too small for the urea to pass through. The molecular weight of urea is 60.06 g/mol‚ over three times greater than the 20 MWCO. 3. Glucose was able to diffuse through the 200 MWCO pore because it was small enough‚ having a molecular weight of 180.2 g/mol. Albumin was unable to pass through because it was far too large‚ having a molecular
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incorrect. I think the urea was not able to diffuse through the 20 MWCO membrane because the molecular weight of urea is about 60. This means it is above the molecular weight cut off and cannot diffuse. 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? a. The glucose was able to diffuse because its molecular weight was less than 200‚ while the albumin was much larger than 200 and could not diffuse
Free Diffusion Molecular diffusion Sodium chloride
connected with electromagnetic radiation. The most important will be the field of molecular imaging that is understood as the visualization‚ characterization and measurement of biological processes at the molecular and cellular levels in humans and other living systems. The techniques used for it include radiotracer imaging/nuclear medicine‚ magnetic resonance imaging (MRI)‚ optical imaging‚ ultrasound and others. Molecular imaging that is now used mostly in scientific laboratories will be probably
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Identifying Unknown Bacteria Using Biochemical and Molecular Methods Beginning of Instructor Pages Instructor Pages - - 3 Purpose The purpose of this lab is to introduce a variety of lab techniques to students working on the common problem of identifying an unknown bacterium. This lab helps students develop an understanding of the biochemical and molecular differences in bacteria and introduces the concept of identifying species based on characeristic gene sequences. Students work through
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in the sample. Western blotting is used during this procedure to test that proteins can be indicators of genetic and evolutionary similarity. Results show that the different species of fish contain the myosin light chains that are equivalent in molecular mass‚ which then means they are similar in their evolutionary relationships. Western Blotting is used to Identify a Subunit of Myosin Light Chain in the Proteins of All the Different Fish. Introduction Western blotting is a technique in biological
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Results(Analysis Questions): Dyes: #4‚ #6‚ and #10 traveled the fastest because they contained a protein with a molecular weight that was smaller than the others. The dye mixtures contained a sample of DNA‚ RNA or other protein. These were negatively charged so that they traveled toward the cathode. There were twelve samples in all‚ labeled #1-#12. Agarose
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