Expressing and Purifying the Recombinant form of Green Fluorescent Protein (rGFP) from the E.coli strain using Ni2+ agarose affinity chromatography technology Abstract The purpose of this experiment was to express and purify the his6-tagged recombinant form of GFP (rGFP) from the organism E.coli using Ni2+ agarose affinity chromatography. The expression of rGFP was confirmed qualitatively using the UV light and was expressed in the E.coli strain BL21 (DE3) (-- removed HTML --) (-- removed HTML
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vertical glass tube over the top of which a stream of vapor-free gas is passed. A water bath is provided for maintaining a steady temperature so that there is no eddy current in the vertical tube and mass transfer takes place from the surface by molecular diffusion alone. The rate of evaporation can be followed by the rate of fall of the liquid surface. A traveling microscope is provided for determining‚ the liquid fall. With the knowledge of the concentration gradient‚ the diffusivity of the vapor
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The purpose of this study is to produce recombinant DNA molecules to produce bacteria that would transform into red fluorescent proteins. One plasmid was that allowed to express a red fluorescence was produced by recombining two plasmids by using molecular techniques. Agar plates labeled LB‚ LB/AMP‚ and LB/AMP/ARA containing ampicillin (AMP) and arabinose (ARA) were used to grow of the bacteria of interest and SDS-PAGE gels were utilized in identifying the fluorescent and non-fluorescent proteins
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communicate the information necessary to make the idea a reality. In order to generate a 3D model‚ designs must start with sketches that are generated within the CAD program. These computer generated sketches will appear resemble hand drawn sketches in geometry (the combination of points‚ lines‚ and shapes)‚ but have big advantages over hand drawn sketches. One important difference between a freehand sketch and a CAD sketch is accuracy. The lines of a CAD sketch can be drawn perfectly straight‚ with start
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Electronics. Answer X1 = Number of GE45 televisions produced per shift X2 = Number of GE60 televisions produced per shift MAX 50X1 + 75X2 S.T. 2X1 + 2X2 300 (Production hours) X1 + 3X2 240 (Assembly hours) X1‚ X2 0 Recap of Analytic Geometry
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EXAM QUESTIONS 1) Describe the alternative fates of pyruvate in cellular respiration. 2) Write notes on the structure and significance of α and β glycosidic bonds. 3) Describe the mechanism of DNA duplication. Include a brief account of the types of proteins‚ cofactors and enzymes involved. 4) Write an account of two of the key historical experiments that identified DNA as the carrier of the genetic code. 5) Describe the “central dogma” for the genetic code and the basis by which genetic information
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Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss‚ Inc. ISBNs: 0-471-37972-7 (Paper); 0-471-22390-5 (Electronic) 12 Electrophoresis Martha L. Booz Chemical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . What Is the Safest Approach to Working with Acrylamide? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . What Are the Symptoms of Acrylamide Poisoning? . . . . . . What
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Make Recombinant DNA? Recombinant DNA Technology May Allow Us To: • Cure or treat disease • Genetically modify our foods to increase flavor‚ yield‚ nutritional value or shelf-life • Better understand human genetics • Clone cells or organs Molecular Biology’s Best Friends: Bacteria Why use bacteria? • They’re relatively simple organisms. • They reproduce very quickly and asexually (this means that the “daughter” cells will contain the exact same DNA as the “parent” cell). • It’s pretty easy
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DNA DIGESTION AND ELECTROPHORESIS In this experiment we will be doing a process called as DNA digestion or also known as restriction digest. A restriction digest is a procedure used in molecular biology to prepare DNA for analysis or other processing. It is sometimes termed DNA fragmentation‚ scientists Hartl and Jones describe it this way: This enzymatic technique can be used for cleaving DNA molecules at specific sites‚ ensuring that all DNA fragments that contain a particular sequence have the
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organisms) is used when we refer to crop plants using different molecular biology techniques or methods to change their properties‚ such as to enhance their resistance to herbicides‚ improve nutritional content‚ and other properties. This seems great‚ but why there are people who disapprove the development and consumption of GM foods? Modifying plant genetics can have many benefits‚ because of the use of precise and accurate molecular techniques‚ the main difference between traditional breeding techniques
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