this experiment‚ the method of Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) was conducted using a negatively charged protein and pre-stained molecular weight markers. The hypothesis was that the molecular weight of N-acetyl-β-D-hexosaminidase B would be 28‚000 kDa. To confirm or reject the hypothesis‚ the molecular weight of N-acetyl-β-D-hexosaminidase B and the concentration of protein had to be determined. The electrophoresis of the protein gel were conducted using a Hoefer
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polyacrylamide gel as a support medium and sodium dodecyl sulfate (SDS) to denature the proteins. The method is called sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) where one can obtain information about the size of a protein or its molecular weight and yield or the total amount of the protein. This system is also called the Laemmli method after U.K. Laemmli‚ who was the first to publish a paper employing SDS-PAGE in a scientific study. SDS (also called lauryl sulfate) is an anionic
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Introduction: Escherichia coli (E.coli) is a commensal-pathogenic organism which include a wide range of strains. There are several advanced molecular-genomic technologies that are capable for detecting and identifying different strains of E.coli. But‚ ERIC-PCR technique is a quick and cost effective method for determining individual strains via demonstration of strain specific fingerprint bands. Therefore‚ the ERIC-PCR technique was used to determine the isolated strains of E.coli from different
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preparation. The molecular weight of the fibrinolytic protease was determined by comparison of the migration distances of standard marker proteins (Lysozyme 14.4 kDa‚ Soybean Trypsin Inhibitor 21.5 kDa‚ Carbonic anhydrase 31.0 kDa‚ Ovalbumin 45.0 kDa‚ Bovine Serum Albumin 66.2 kDa and Phosphorylase b 97.4 kDa). Depending on the relative mobility‚ the molecular weight of the protein band was calculated to be around 31 kDa. Thus‚ it was concluded that our fibrinolytic protease enzyme has a molecular weight of
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knowledgeable the risk from possible exposure to blood and body fluids and that is why a lot of people today have turned to Diagnostic Sonography. Diagnostic Sonography has turned the medical field because unlike X-rays‚ Sonography is a radiation-free imaging procedure. Working with patients
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converted to a percentage grade when registered in your “Gradebook”. |ACTIVITY 1: Simulating Dialysis (Simple Diffusion) |Answers | |The molecular weight of urea is approximately 60. Which of the membranes can it pass through? |D | |50 MWCO membrane
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BIODEL INC. EXECUTIVE SUMMARY Biodel’s use of biotechnology expertise in the three prime areas of Cell Biology‚ Molecular Biology‚ and Immunodiagnostics has led their company into some great opportunities. Biodel created a synthetic serum to replace fetal calf serum and horse serum‚ and provide a more consistently available product for scientific researchers. Biodel’s Molecular Biology division used DNA technology to gain 60% of the market share of growth factors and stock the largest collection
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EXPERIMENT NO. 15 PROTEIN CHARACTERIZATION BY ELECTROPHORESIS Abstract The molecular weights of protein extracts were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two sets of four protein samples‚ standard bovine serum albumin (BSA)‚ invertase‚ egg albumin‚ and casein‚ were prepared; one set containing β-mercaptoethanol (BME) while the other did not. These were then analyzed through SDS-PAGE with 12.5% resolving gel‚ prepared using 2 M Tris-HCl at pH 8
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The effect of the molecular weight to the rate of diffusion Based on the experiments‚ sd f f f f s wrefwe fwe rfwjv rwefhnw fwer fwe fwef fwe fwe fwe fwef wef w fwe fwe From Wikipedia‚ the free encyclopedia Jump to: navigation‚ search Sucrose is the organic compound commonly known as table sugar and sometimes called saccharose. This white‚ odorless‚ crystalline powder has a pleasing‚ sweet taste. It is best known for its role in human nutrition. The molecule is a disaccharide derived from glucose
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Molecular Geometry A. Natural Orientation of Volumes about a Central Point. You will need 20 round balloons for this experiment. Join them together as indicated in the Balloon Arrangement column and then describe the shape in the space provided. Balloon Arrangement Description of the Shape Two-Balloon Set Linear Three-Balloon Set Trigonal Planar Four-Balloon Set Tetrahedral Five-Balloon Set Trigonal Bipyramidal Six-Balloon Set Octahedral B. Valence Shell Pairs: Single
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