standard ciprofloxacin. At the same time the analogue was retaining antibacterial activity towards gram positive species when compared to standard ciprofloxacin. The molecular docking studies showed a good correlation between their antibacterial activity and autodock binding free energy. Keywords: Ciprofloxacin‚ 1‚2‚4-Triazole‚ Molecular docking‚ Antimicrobial Introduction Quinolones have become a major class of antibacterial agents‚ which are under extensive clinical development. They have an attraction
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Since‚ the molecular weight of urea is 60.07‚ the molecules of urea were too large to enter the pores of the 20 MWCO. 4. Describe the result of the attempts to diffuse glucose glucose and albumin through the 200 MWCO membrane. How well did the results compared with your predictions? Glucose could diffuse through the 200 MWCO while albumin could not diffuse through such membrane. It was because there was difference of molecule weight between them. Glucose (C6H12O6) has a molecular weight of
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concentration gradient. The molecular size vs the MWCO size of the membrane can either increase‚ decrease‚ or prevent diffusion. The greater the concentration gradient the greater the diffusion rate due to molecules moving from areas of higher concentration to areas of lower concentration. • Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? My prediction was correct. The molecular weight of urea is 60.07 and
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analyze and identify DNA by use of a spectro-photometer * to use restriction enzymes to cleave DNA into fragments * to visualize the restriction fragments by gel electrophoresis * to compare the different DNA fragments generated by use of molecular markers Abstract This work describes a lysis method for the isolation and purification of bacterial genomic DNA and visualization of the restriction fragments by agarose gel electrophoresis. It was noted that for one to isolate and purify bacterial
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through the 20 MWCO because the pores of the membrane were too small for the urea to pass through. The molecular weight of urea is 60.06 g/mol‚ over three times greater than the 20 MWCO. 3. Glucose was able to diffuse through the 200 MWCO pore because it was small enough‚ having a molecular weight of 180.2 g/mol. Albumin was unable to pass through because it was far too large‚ having a molecular
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incorrect. I think the urea was not able to diffuse through the 20 MWCO membrane because the molecular weight of urea is about 60. This means it is above the molecular weight cut off and cannot diffuse. 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? a. The glucose was able to diffuse because its molecular weight was less than 200‚ while the albumin was much larger than 200 and could not diffuse
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ROLE OF BACTERIOPHAGES IN GENETICS AND MOLECULAR BIOLOGY INTRODUCTION AND DISCOVERY: A bacteriophage is any one of a number of viruses that infect bacteria. Bacteriophages are among the most common biological entities on Earth. The term is commonly used in its shortened form‚ phage.Typically; bacteriophages consist of an outer protein capsid‚ enclosing genetic material. The genetic material can be ssRNA‚ dsRNA‚ ssDNA‚ or dsDNA along with either circular or linear arrangement. Bacteriophages are
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Identifying Unknown Bacteria Using Biochemical and Molecular Methods Beginning of Instructor Pages Instructor Pages - - 3 Purpose The purpose of this lab is to introduce a variety of lab techniques to students working on the common problem of identifying an unknown bacterium. This lab helps students develop an understanding of the biochemical and molecular differences in bacteria and introduces the concept of identifying species based on characeristic gene sequences. Students work through
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in the sample. Western blotting is used during this procedure to test that proteins can be indicators of genetic and evolutionary similarity. Results show that the different species of fish contain the myosin light chains that are equivalent in molecular mass‚ which then means they are similar in their evolutionary relationships. Western Blotting is used to Identify a Subunit of Myosin Light Chain in the Proteins of All the Different Fish. Introduction Western blotting is a technique in biological
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REQUEST FOR PROPOSAL INTEGRATED RADIOLOGY INFORMATION SYSTEM (RIS) AND PICTURE ARCHIVAL COMMUNICATIONS SYSTEM (PACS) FOR Your Hospital Name Here File No.: _____________________ Solicitation No: _______________ Proposal Opening Date:___________ Proposal Opening Time:___________ Table of Contents Table of Contents 1 REQUEST FOR PROPOSAL 3 PART I. ADMINISTRATIVE AND GENERAL INFORMATION (REV. 8/07) 3 1.1 Background 3 1.1.1 Purpose 5 1.1.1.1 Table of
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