Simple Diffusion Activity 1: Simulating Simple diffusion 1. What is the molecular weight of Na+? 22.99 2. What is the molecular weight of Cl-? 35.45 3. Which MWCO dialysis membranes allowed both of these ions through? 50‚ 100‚ and 200 4. Which materials diffused from the left beaker to the right beaker? NaCl‚ Urea‚ and Glucose at MWCO 200 5. Which did not? Why? Albumin‚ too large to diffuse Activity 2: Simulating Dialysis 1. What happens to the urea concentration in the left beaker
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Chapter 4 Carbon and the Molecular Diversity of Life Lecture Outline Overview: Carbon – The Backbone of Biological Molecules * Although cells are 70–95% water‚ the rest consists mostly of carbon-based compounds. * Carbon is unparalleled in its ability to form large‚ complex‚ and diverse molecules. * Carbon accounts for the diversity of biological molecules and has made possible the great diversity of living things. * Proteins‚ DNA‚ carbohydrates‚ and other molecules that
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WESTERN BLOTTING (Written Report) I. PRINCIPLE Western blotting is a method for identifying a specific protein in a complex mixture and simultaneously determining its molecular weight. In Western blotting‚ proteins are electrophoresed into a gel‚ as the proteins migrate through the gel they are separated based upon size and charge. Characteristically‚ smaller proteins migrate through the gel faster than larger proteins. Sufficiently separated proteins in an SDS-PAGE can be transferred to a
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NEW AND RESTRUCTURED POST-GRADUATE CURRICULA & SYLLABI Plant Sciences Genetics & Plant Breeding Seed Science & Technology Education Division Indian Council of Agricultural Research New Delhi April 2009 Contents Page(s) Executive Summary 3-4 BSMAC Composition 5 Preamble 6-8 Organization of Course Contents & Credit Requirements 9 Genetics & Plant Breeding 10-50 Course Structure – at a Glance Course Contents List of Journals e-Resources Suggested Broad
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gave ions to support the conductivity for the gel to run. The uncut λ DNA would be added to labeled tube as a control DNA. By comparing the conformations of uncut DNA with the cut DNA‚ the completed digestion could be verified. Molecular marker‚ which was used for molecular weight calculation‚ was stored in another sample tube. The prepared sample contained puC18 DNA would be run as the degrade DNA control
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Lab Report One Exercise Five Activity One “Simulating Simple Diffusion” Activity Two “Simulating Facilitated Diffusion” Jessica Ogola Anatomy and Physiology 2401 Dr. Denyse Jones 02/02/2012 Objective: The objective of this experiment is to perform the simulation of the movement of solutes from a higher concentration to a lower concentration within a given amount of time. The goal of the exercise is to provide a simulation for the process of the facilitated diffusion of
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Gel Filtration Gel filtration is a non-adsorptive chromatography technique that separates molecules on the basis of molecular size. Desalting and buffer exchange are two special examples of gel filtration that are widely used in many downstream bioprocesses. Desalting is used to completely remove or lower the concentration of salt or other low molecular weight components in the sample while buffer exchange replaces the sample buffer with a new buffer. Gel filtration is one of the easiest chromatography
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concentration values explain the bands thickness and color. The smear lastly is Gamma globulin although it has a close concentration as alpha 1 and beta globulin it has smeared zone and that’s due to its light molecular weight‚ so we conclude that another reason for the band thickness is the molecular weight of the
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1958‚ where he became a professor at McMaster University in Hamilton‚ Ontario. Developed in 1957 with Ronal Nyholm‚ Gillespie has done extensive work on expanding the idea of the VSEPR model of molecular geometry. The theory they created is much more effect to predict‚ explain and describe the 3D molecular shapes (linear‚ pyramidal‚ cubical etc.) based on the number of electron pairs that are found on the outer shell. Their theory is based on electron repulsion of bonded and unbonded electron pairs
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BIO MOLECULAR LABORATORY REPORT BIO 615 Name: NUR LISMA RUHILA BT ALIAS Group: AS201 5A Experiment: GEL ELECTROPHORESIS OF EXTRACTED DNA 0.5% AGAROSE GEL Group partners: 1) HALIMATUN SAADIAH BT MOHD BUSTAMAM 2) NUR FARHANA BT AHMAD SOPIAN 3) FATIN NUR ASYIQIN BT ABD TALIB 4) UMMU AFIQAH BT HASSAN 5) NABIHAH BT MD NAWAWI Date of experiment: 8th October 2012 Date of submission: 15th October 2012 TITLE: GEL ELECTROPHORESIS OF EXTRACTED DNA 0.5% AGAROSE GEL
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