nucleic acids and proteins. Electrophoresis can take a mixture of macromolecules of different molecular weights‚ shapes‚ and various electrical charges to determine all the various compounds in the mixture and allowing for further purification that can aid in details of individual elements of the mixture being studied. Agarose gel electrophoresis is a very important technique used in the field of molecular science. The field of forensics utilizes agarose gel electrophoresis by allowing investigators
Premium Gel electrophoresis Molecular biology Protein
MWCO membrane. A possible reason to why none of the of these solutes were able to pass through was because the solutes could have been too big to be transported. According to your results‚ which solute had the highest molecular weight? The solute that had the highest molecular weight was Albumin. Which solute displayed the highest rate of diffusion through the 200 MWCO membrane? The solute that displayed the highest rate of diffusion through the 200 MWCO
Premium Chemistry Diffusion Osmosis
crystal (Methylene blue‚ solid or Potassium Permanganate KMnO4-purple) will move further than the other due to the driving force (kinetic energy)? My prediction is that Potassium Permanganate KMnO4- purple (molecular mass- 158.03 g/mol) will move further than Methylene blue‚ solid (molecular mass- 319.85 g/mol) since it weighs less. In osmosis is diffusion of H2O across a selectively permeable membrane (dialysis tubing). There is only movement of water‚ not solute‚ across the plasma membrane. Water
Premium Diffusion Molecular diffusion Osmosis
Characterization of a Yam Class IV Chitinase Produced by the Recombinant Pichia pastoris X-33 Department of Botany‚ Jahangirnagar University‚ Savar‚ Dhaka 1342‚ Bangladesh Abbreviations: Endo H‚ Endoglycosidase H; PR protein‚ pathogenesis related protein; P. pastoris‚ Pichia pastoris; VTS‚ vacuolar targeting signal; PCR‚ polymerase chain reaction; PAGE‚ polyacrylamide gel electrophoresis; SDS‚ sodium dodecyl sulfate Abstract A yam (Dioscorea opposita Thunb) class IV chitinase‚ whose genomic
Premium Molecular biology Protein DNA
References: M. F. Rubner‚ Molecular Electronics‚ Research Studies Press‚ 1992‚ chapter 2 Luis Alcacer‚ Conducting Polymers‚ D. Reidel Publishing Company‚ 1987 Herbert Naarmann‚ Polymers to the Year 200 and Beyond‚ John Wiley & Sons‚ 1993‚ Chapter 4 Ian M. ‚ Introduction to Synthetic
Premium Polymer Plastic
capital project proposal is for a Magnetic Resonance Imaging (MRI) compatible monitor and a program for sedation services for patients. Nursing support is necessary for monitoring patients during magnetic imaging for patients receiving oral and intravenous conscious sedation or pain management. In some cases anesthesia support may be necessary and a physician or radiologist to provide orders and supervision for needed sedation to complete imaging. Several studies reveal that 14% to 20% adults need
Premium Physician Hospital Management
Expressing and Purifying the Recombinant form of Green Fluorescent Protein (rGFP) from the E.coli strain using Ni2+ agarose affinity chromatography technology Abstract The purpose of this experiment was to express and purify the his6-tagged recombinant form of GFP (rGFP) from the organism E.coli using Ni2+ agarose affinity chromatography. The expression of rGFP was confirmed qualitatively using the UV light and was expressed in the E.coli strain BL21 (DE3) (-- removed HTML --) (-- removed HTML
Premium DNA Bacteria Escherichia coli
vertical glass tube over the top of which a stream of vapor-free gas is passed. A water bath is provided for maintaining a steady temperature so that there is no eddy current in the vertical tube and mass transfer takes place from the surface by molecular diffusion alone. The rate of evaporation can be followed by the rate of fall of the liquid surface. A traveling microscope is provided for determining‚ the liquid fall. With the knowledge of the concentration gradient‚ the diffusivity of the vapor
Premium Liquid Diffusion Water
The purpose of this study is to produce recombinant DNA molecules to produce bacteria that would transform into red fluorescent proteins. One plasmid was that allowed to express a red fluorescence was produced by recombining two plasmids by using molecular techniques. Agar plates labeled LB‚ LB/AMP‚ and LB/AMP/ARA containing ampicillin (AMP) and arabinose (ARA) were used to grow of the bacteria of interest and SDS-PAGE gels were utilized in identifying the fluorescent and non-fluorescent proteins
Premium DNA Molecular biology
http://www.mhhe.com/physsci/chemistry/essentialchemistry/flash/hybrv18.swf Salient Features Chemistry animation and videos Atomic orbital animations Hybridization animation http://jchemed.chem.wisc.edu/JCEDLib/WebWare/collection/open/JCEWWOR019/mo Molecular orbital theory animations movies.html http://www.mhhe.com/physsci/chemistry/essentialchemistry/flash/activa2.swf http://dwb4.unl.edu/chemAnime/Electro.htm Activation energy animation Electrochemistry animations RW-7 RW-8 RW-9 http://www.mhhe
Free Chemical bond Covalent bond Ionic bond