EXAM QUESTIONS 1) Describe the alternative fates of pyruvate in cellular respiration. 2) Write notes on the structure and significance of α and β glycosidic bonds. 3) Describe the mechanism of DNA duplication. Include a brief account of the types of proteins‚ cofactors and enzymes involved. 4) Write an account of two of the key historical experiments that identified DNA as the carrier of the genetic code. 5) Describe the “central dogma” for the genetic code and the basis by which genetic information
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Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss‚ Inc. ISBNs: 0-471-37972-7 (Paper); 0-471-22390-5 (Electronic) 12 Electrophoresis Martha L. Booz Chemical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . What Is the Safest Approach to Working with Acrylamide? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . What Are the Symptoms of Acrylamide Poisoning? . . . . . . What
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Make Recombinant DNA? Recombinant DNA Technology May Allow Us To: • Cure or treat disease • Genetically modify our foods to increase flavor‚ yield‚ nutritional value or shelf-life • Better understand human genetics • Clone cells or organs Molecular Biology’s Best Friends: Bacteria Why use bacteria? • They’re relatively simple organisms. • They reproduce very quickly and asexually (this means that the “daughter” cells will contain the exact same DNA as the “parent” cell). • It’s pretty easy
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DNA DIGESTION AND ELECTROPHORESIS In this experiment we will be doing a process called as DNA digestion or also known as restriction digest. A restriction digest is a procedure used in molecular biology to prepare DNA for analysis or other processing. It is sometimes termed DNA fragmentation‚ scientists Hartl and Jones describe it this way: This enzymatic technique can be used for cleaving DNA molecules at specific sites‚ ensuring that all DNA fragments that contain a particular sequence have the
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organisms) is used when we refer to crop plants using different molecular biology techniques or methods to change their properties‚ such as to enhance their resistance to herbicides‚ improve nutritional content‚ and other properties. This seems great‚ but why there are people who disapprove the development and consumption of GM foods? Modifying plant genetics can have many benefits‚ because of the use of precise and accurate molecular techniques‚ the main difference between traditional breeding techniques
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My graduate studies dealt with extensive and comprehensive advanced coursework and research‚ which not only helped me learn and understand the intricacies of Microbiology and Molecular Biology‚ but also provided an insight of its association with other fields like Biochemistry and Bioinformatics. I completed my graduate research work under the guidance of Dr. Shaikh‚ who is an expert in the field of Enzymology and Virology. The
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PUNNETT SQUARE/EXPLANATION Punnett Square F f f Ff ff f Ff ff Because this disorder is a dominant autosomal‚ the unaffected woman would have a recessive allele. Crossing the two‚ these would be the results. They had a child that would be FH‚ as descripted. ANIMATION NOTES Restriction enzymes bind/target to specific DNA sequences As they bind to the DNA sequence‚ the restriction enzymes cut the sugar-phosphate backbones of the DNA EcoRi is a restriction enzyme EcoRI cuts unevenly
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Permeability Simple Diffusion The following refer to Activity 1: Simulating Dialysis (Simple Diffusion). Which solute(s) were able to pass through the 20 MWCO membrane? NONE 1. According to your results‚ which solute had the highest molecular weight? Albumin 2. Which solute displayed the highest rate of diffusion through the 200 MWCO membrane? Na+Cl 3. Using the data from Chart 1‚ explain the relationship between the rate of diffusion and the size of the solute. The larger
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Activity 1: 1. Did any solutes move through the 20 MWCO membrane? Why or Why not? 2. Did Na+Cl- move through the 50 MWCO membrane? Activity 2: 1. Are the solutes moving with or against their concentration gradient in facilitated diffusion? 2. What happened to the rate of facilitated diffusion when the number of carrier proteins was increased? 3. In the simulation you added Na+Cl- to test its effect on glucose diffusion. Explain why there was no effect. Activity 3: 1. Which membrane resulted
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Nanotechnology Applications Nanotechnology is science that involves working with matter at the molecular or nano-scale (25‚ 400‚ 000 nanometers equal 1 inch) dimension‚ the understanding and control of matter at dimensions between 1 and 100 nanometers (AmericanElements‚ 2010). Today where unique phenomena enable novel applications‚ this includes Fuel Cell Development‚ Biotechnology‚ and Medicine among others. Using the heating properties of iron oxide nanoparticles in gene regulation‚ cancer therapy
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