of hypervariable regions using photographic film to detect the radioactivity. Figure I - A Photograph Of Dna Fingerprints Of Five Individuals (From Science and Technology in Society‚ The Association of Science Education‚ Hatfield‚ England.) Molecular Genetics Unit Part I - The Fingerprinting
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sciences. During my period of study here‚ I have taken courses like Biochemistry‚ Molecular Cell Biology‚ Microbiology‚ Immunology‚ Bioprocess principles and engineering‚ Bio and chemical thermodynamics‚ Enzyme engineering. These courses took me a step closer towards fulfillment of my goal of delving deeper into the world of ‘cloned Dollies’ and ‘Docile DNA’. The fascinations and promises held out by various areas in molecular biology‚ biochemistry and
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Simple Diffusion Activity 1: Simulating Simple diffusion 1.What is the molecular weight of Na+? 22.9 2. What is the molecular weight of Cl-? 35.45 3. Which MWCO dialysis membranes allowed both of these ions through? 50‚ 100‚ and 200 4. Which materials diffused from the left beaker to the right beaker? Urea‚ NaCl and glucose diffused 5. Which did not? Why? Albumin‚ because the molecular weight exceeded the highest MWCO membrane‚ thus being to large to pass through
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credits = total hours) 1. Lecture 1 (4 hours): Introduction to the course. History; Applications; Biotechnology today. 2. Lecture 2 (4 hours): Cell and molecular biotechnology. Prokaryotic and eukaryotic cells; molecules in the cells. The central dogma of molecular biology; replication‚ transcription and translation; The principles of molecular biology and applications. 3. Lecture 3 (4 hours): Basic principles of immunology and applications. Natural and acquired immunity; cell- and
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a molecular sieve is created such that the distance traveled in the gel toward the anode by any DNA fragment (all of which carry negatively charged phosphate groups in the presence of a basic buffer) is inversely proportional to its molecular weight. Further‚ such distance traveled has a linear relationship with the log of such fragment’s molecular weight. Since DNA consists solely of deoxyribonucleotides that differ only by their bases and each base pair has approximately the same molecular weight
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opposed to those performed ten years ago and analyse its future implications. The thrust of research while studying biology at a molecular level initially was focused specifically on the genomes of various organisms. As scientists discovered the intricacies of genes and their functionalities‚ the attention was soon drawn towards the end result of the central dogma of molecular biology‚ namely‚ the proteins‚ produced through translation of RNAs. Therefore‚ to study the proteins produced in an organism
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band‚ spacing pattern of bands) you collected for the samples (fish‚ crab‚ food‚ protein ladder) in the appropriate columns. The approximate molecular weight of a band is determined by comparing the band of the food sample to the protein ladder bands. Sample Fish (1) Crab (2) Food (3) Protein Ladder (M) Number of Bands 5 2 2 6 Approximate Molecular Weight of the Bands Spacing Pattern of Bands 1 separated from 2 pair of bands spaced apart 2 bands at 45 and 29 kd 2 bands at 45 and
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BIOLOGY 1201‚ Section #2 Spring‚ 2014 (MWF 9:30-10:20 AM) Instructor: Dr. Terry M. Bricker Office: A606 Life Science Annex (Laboratory‚ A623 – check the lab if I am not in the office!) Phone: 225-578-1555 E-mail: btbric@lsu.edu -- E-mail is an excellent way to contact me or ask questions. Office Hours: M‚Tu 3:30-4:30‚ or by appointment. - Please see me if you have questions or problems. COURSE: BIOL 1201‚ General Biology‚ 3 credit hours. Biology 1201 is a course intended for students that are majoring
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Restriction Fragment Length Polymorphism and Southern Blotting 1. Abstract The aim of the experiment was to be introduced to the techniques involved in the identification of restriction fragment length polymorphisms. Restrictions were carried out using three different restriction enzymes‚ ECORI‚ HindIII and BstELI with their buffers. Lambda (λ) DNA was then examined using electrophoresis and Southern blotting. The results showed that λ DNA was best digested by EcoRI as all of the expected bands
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Nanotechnology is the engineering of functional systems at the molecular scale. This covers both current and work and concepts that are more advanced. Nanotechnology is the projected ability to construct items from the bottom up‚ using techniques and tools being developed to make complete‚ high performance products. Basically‚ nanotechnology is a field of research concerned with building things (materials and devices). What it does: increases efficiency of energy consumption‚ helps clean the environment
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