Dr. Randy Schekman graduated from the university of California‚ LA in 1971 for molecular biology and got his Ph.D. in biochemistry at Stanford University in 1975. Now he is currently working at the university of California as a professor of cell and development biology and also works at the Howard Huges Medical Institute Investigator. His main focus for his research is in the molecular description of the process of membrane assembly and vestibular movement in the eukaryotic cells. His interest
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Simple Diffusion Activity 1: Simulating Simple diffusion 1. What is the molecular weight of Na+? 22.99 or 23 2. What is the molecular weight of Cl-? 35.45 3. Which MWCO dialysis membranes allowed both of these ions through?50‚100‚ 200 4. Which materials diffused from the left beaker to the right beaker? Urea‚ NaCl and glucose diffused 5. Which did not? Why? Albumin was too large to diffuse into the right beaker. Activity 2: Simulating Dialysis 6. What happens to the urea concentration
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1. SDS-PAGE of purified protein X. Lane 1‚ Protein ladder (in Daltons). Lane 2‚ purified protein X (affinity chromatography). Lane 3‚ purified protein X (company manufactured). Lane 4‚ elution buffer. a. What is the benefit of a protein ladder/molecular weight marker in an SDS-PAGE gel? Describe what you can learn about the protein bands found in lanes 2‚ 3 and 4 based on the protein ladder bands. b. Positive and negative controls are absolutely essential to the validity of experimental data. In
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Title: Diffusion throughout the membranes Lab Partner(s): Alexis Clouting Date: 2/15/15 Abstract: In the content of the Module 2 we learned about Diffusion across cell membranes. We touched on the different types of cells and their functions. How things are transported in and out of cells. Learning about isotonic‚ hypertonic and even hypotonic solution. This is not my first time touching on this subject in my nursing career and I learned a way to remember what happens in the different solutions
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(Appendix A). 1mL of the dye solution was added to 20uL of the protein sample‚ mixed and incubated for 10 minutes at room temperature before measuring the absorbance at 595nm using the spectrophotometer. 3.6 Verification for the Homogeneity and Molecular Weight of the DNA Polymerase
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think the urea was not able to diffuse through 20 MWCO? How well did the results compare with your predictions? The urea was not able to diffuse through the 20 MWCO because the membrane pores are too small and the urea molecules are large. The molecular weight of Urea is 60.07 g/mol which is too large thus the molecules were not able to pass through the pores of the 20 MWCO. The results that I obtained from the experiment agreed with these predictions because urea was no able to diffuse through
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ADVANCED IMAGING ( MIDTERM) MARCH 04‚ 2012 Please read each question carefully and select the best answer 1. During the angiographic procedure‚ oxygen should be applied to the patient when the oxygen saturation level falls below: a. 50% b. 75% c. 60% d. 90% 2. The symbol used to denote that oxygen has been bound to hemoglobin to form oxyhemoglobin is: a. HbO2 b. SbO2 c. Hb2O2 d. SpO 3. A heart rate of less than 60 beats/ minute would be considered to be: a. Tachycardia b. Normal
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IPTG induction‚ salting out‚ thermostable polymerase Background: Taq polymerase is a thermostable enzyme essential in the PCR reaction that is isolated from recombinant E. coli. Results: Taq polymerase was isolated‚ the sample was not pure Taq‚ molecular weight found to be 114kDa. Conclusion: The methods isolated taq but an improvement of cation exchange and increase heat denaturation should be added. Significance: An improved method of Taq isolation offers a valuable resource for PCR. SUMMARY The
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By RF was finding the molecular weight of the enzyme. The molecular weight of 6-PGD is 44 KDa. Studying properties (characterization) of the enzyme while the enzyme is more active in optimum temperature at 60°C‚ in Tris-HCl PH=8. 0 shown in the figure (4.4.) high activity. The optimum ionic strength
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vector was used to clone the individual PCR products which then transformed into E. coli. Catalytically active domains were studied by analyzing deduced amino acid sequences of genes. SDS-PAGE analysis was carried out to estimate the molecular weights of proteins. Molecular weights of peh‚ celB and celC products were found to be 41.5 kDa‚ 29.5 kDa and 40 kDa‚ respectively. Agar diffusion assays were performed to qualitatively determine the activities of polygalacturonase and cellulase of the cloned
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