UNIVERSITY OF HULL MODULE NO: 58372 Laboratory based skills 4 Noel Bacasmas Student no: 201012397 BSc Applied Biomedical Science (Part time) Contents Page No. Title and learning objectives.....................................................................3 Introduction.............................................................................................5 Mechanisms of Fibrinolysis.......................................................................6 Different
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DRUGS TO TREAT CANCERS & DRUGS TO TREAT NAUSEA AND VOMITING OBJECTIVES 1. State the rationale for the use of antineoplastic agents in the treatment of cancer. Antineoplastic agents (cell cycle specific or cell cycle nonspecific) target rapidly growing cells – malignant cells rapidly divide (uncontrolled growth) and are unable to repair DNA damage. Also used as adjuvant for post-excision of tumor to prevent recurrence of cancer. 2. Outline the phases of the cell division cycle. G0 Phase:
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Sciences of the United States of America‚ 68‚ 820 - 823. KNUDSON‚ A. G. 2001. Two Genetic Hits (More or Less) to Cancer. Nature Reviews Cancer‚ 1‚ 157 - 162. MOLINA‚ M. A. & ET-AL 2001. Trastuzumab (Herceptin)‚ a Humanized Anti-HER2 Receptor Monoclonal Antibody‚ Inhibits Basal and Activated HER2 Ectodomain Cleavage in Breast Cancer Cells. Cancer Research‚ 61‚ 4744. TAN‚ A. R. 2003. Ongoing adjuvant trials with trastuzumab in breast cancer. Seminars in Oncology 30‚ 54 - 64. TODD R‚ W. D. 1999. Oncogenes
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lines is fundamental to the manufacture of viral vaccines and other products of biotechnology Biological products produced by recombinant DNA (rDNA) technology in animal cell cultures include enzymes‚ synthetic hormones‚ immunobiologicals (monoclonal antibodies‚ interleukins‚ lymphokines)‚ and anticancer agents. Although many simpler proteins can be produced using rDNA in bacterial cultures‚ more complex proteins that are glycosylated (carbohydrate-modified) currently must be made in animal cells
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consisting of production and animal facilities in Shanghai. Abmart’s goal is to enable every protein to have a standard set of monoclonal antibodies optimal for all possible applications. With SEAL™ technology‚ developed in 2011 and a scalable monoclonal antibody development process‚ Abmart will deliver even lower cost and faster discovery time for monoclonal antibodies to more than 1000 customers worldwide. Meeting Style There are basically three different styles of Abmart’s meetings: ‘information
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theory of immunity • 1891‚ Robert Koch demonstrated the cutaneous (delayed-type) hypersensitivity • 1894‚ Richard Pfeiffer‚ Bacteriolysis Historical Perspective (1 of 6 ) • 1895‚ Jules Bordet‚ Complement and antibody activity in bacteriolysis • 1900‚ Paul Ehrlich‚ responsible for the antibody formation theory • 1901‚ Karl Landsteiner‚ A‚ B‚ and O • 1901-8‚ Carl Jensen & Leo Loeb‚ Transplantable tumors • 1902‚ Paul Portier & Charles Richet‚ Anaphylaxis Historical Perspective (1 of 6 ) • 1903
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Myeloma is a form of cancer which affects the plasma cells of the body‚ which are white blood cells. Multiple Myeloma‚ first described in 1848‚ is a disease “characterized by a proliferation of malignant plasma cells and a subsequent overabundance of monoclonal paraprotein.” To understand how Multiple Myeloma affects an infected person’s plasma cells‚ it helps to have a general understanding of how normal blood cells are formed and how they act. Most blood cells develop from stem cells‚ which can be
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A Prion is a normal protein that is found on the membranes of cells. The normal Prion protein (PrPC) consist mainly alpha helix rich 30-35kDa glycoprotein with 209 amino acid sequence and one disulfide bond (1). The disease causing form of normal prion protein named after scrapie (PrPSc) occurs when properly folded normal prion protein change its conformation. Although the exact tertiary structure of PrPSc still remains elusive but the previous studies have shown that the secondary structure of
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Objectives Protein Isolation: Protein isolation for a western blot uses detergents and mechanical force to separate seeded cells from their container. Eukaryotic cells are attached to the surface of a flask by cadherins. In the past‚ we’ve separated the cells from the flask by breaking these bonds with a protease‚ but in order to keep the proteins intact‚ a different method needs to be used to extract the proteins. In protein extraction for a western blot‚ we use detergents to lyse the membrane
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NIH Public Access Author Manuscript Cell Signal. Author manuscript; available in PMC 2010 February 1. Published in final edited form as: Cell Signal. 2009 February ; 21(2): 212–219. doi:10.1016/j.cellsig.2008.10.003. NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author Manuscript Altered EGFR Localization and Degradation in Human Breast Cancer Cells with an Amphiregulin/EGFR Autocrine Loop Nicole E. Willmartha‚b‚ Andrea Baillob‚ Michele L. Dziubinskib‚ Kristy Wilsonc‚ David J
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