overcome this‚ a very common strategy is to use secondary molecules such as antibodies‚ to capture the target protein on the surface. An antibody that recognizes the membrane protein of interest can be immobilized covalently on the surface and subsequently capture the target protein when it is injected. Opposite to covalent immobilization‚ where proteins are immobilized randomly‚ the availability of monoclonal antibodies directed against specific epitopes on the target protein makes it possible to
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FUTURE OF BIOTECHNOLOGY Anna Treohan 1993 Woodrow Wilson Biology Institute INTRODUCTION The recent emphasis on environmental awareness has challenged scientists to find solutions for better and safer living conditions. The added threat of deadly diseases such as AIDS and resistant strains of tuberculosis and gonorrhea have forced scientists to look for new therapies within the field of biotechnology. The structure of DNA was deciphered by James Watson‚ a geneticist‚ and Francis Crick‚ a
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Leukemia is a cancer of blood in which there are too many white blood cells in the bone and the bone marrow. A person’s bone marrow makes abnormal white blood cells‚ which are leukemia cells. Leukemia cells‚ unlike normal blood cells‚ do not die. They may displace normal white blood cells‚ red blood cells and platelets. Thus‚ they can’t do their work. There are several types of leukemia. They are grouped based on how quickly the disease develops and gets worse. The chronic leukemia usually gets
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IMMUNOLOGY QP Code: 7351 Your answers should be specific to the questions asked. Draw neat labeled diagrams wherever necessary. Answer all questions LONG ESSAYS 1. 2. 2 X 20 = 40 Marks Discuss the production‚ properties and uses of monoclonal antibodies Discuss various in vitro antibiotic sensitivity testing methods SHORT ESSAYS 3. 4. 5. 6. 7. 8. Dark ground microscope Enzyme linked immunosorbentassay (ELISA) Type IV Hypersensitivity reaction Endotoxins T – cell subsets Chemical disinfectants
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EFFECTS OF RADIATION Background Radiation is all around us. It is naturally present in our environment and has been since the birth of this planet. Consequently‚ life has evolved in an environment which has significant levels of ionizing radiation. It comes from outer space (cosmic)‚ the ground (terrestrial)‚ and even from within our own bodies. It is present in the air we breathe‚ the food we eat‚ the water we drink‚ and in the construction materials used to build our homes. Certain foods
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pernicious anemia Used to sterilize surgical instruments…and to improve the safety and reliability of industrial fuel oil burners. Used in cancer treatment‚ food irradiation‚ gauges‚ and radiography. When injected with monoclonal antibodies into a cancer patient‚ helps the antibodies bind to and destroy the tumor. Curium-244 Used in mining to analyze material excavated from pits…and slurries from drilling operations. Gallium-67 Used in medical diagnosis Iodine-123 Widely used to
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GATE 2011 Syllabus for Biotechnology Linear Algebra: Matrices and determinants‚ Systems of linear equations‚ Eigen values and Eigen vectors. Calculus: Limit‚ continuity and differentiability‚ Partial derivatives‚ Maxima and minima‚ Sequences and series‚ Test for convergence‚ Fourier Series. Differential Equations: Linear and nonlinear first order ODEs‚ higher order ODEs with constant coefficients‚ Cauchy’s and Euler’s equations‚ Laplace transforms‚ PDE- Laplace‚ heat and wave equations.
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YUDEXYS PENALVER 12/20/2012 ABSTRACT #2-Polyuria PURPOSE: The purpose of the study is to identify polyuria in recipients that have under gone renal transplant and have experience excessive urine output. METHOD: A 57-year-old male patient with hypertension and hyperglycemia was referred to our hospital because of a diagnosis regarding his left adrenal tumor. Because the levels of urinary metanephrine and normetanephrine were elevated‚ and 131I-MIBG scintigraphy showed intense uptake in the
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Purification of Infliximab[3] begins at Stage 1 with the filtration of the harvest material using a spin filter. The harvest is then clarified and undergoes 3 main chromatographic steps. Firstly‚ at Stage 2‚ the direct protein capture by Protein A affinity chromatography helps to remove a large majority of the impurities whicn include viruses and media components. At Stage 3‚ prior to cation exchange chromatography‚ the purified material in the eluted stream is then subjected to freezing‚ thawing
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alternate drug targets. In this review‚ the authors discuss the current situation of antibacterial medicines and present data on new antibiotic targets. Moreover‚ alternatives to antibiotics‚ such as bacteriophages‚ antimicrobial peptides and monoclonal antibodies‚ are presented. The authors also draw attention to the valuable features of natural sources in developing antibacterial compounds. The need to prevent and control infections as well as the reasonable use of currently available antibiotics
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