BSCI422 Immunology January 26‚ 2010 Lecture 1 Introduction to the class Went over syllabus Student expectations BSCI422 Immunology January 27‚ 2010 Lecture 2 Innate Immunity‚ the signal transduction and cytokine production When you’re exposed to a microbe‚ your body has innate immune responses that can kill the microbe right away‚ there is no recovery or memory period. It is the very first response. If you amount an affective innate immune response‚ you don’t need to develop an adaptive
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binding assays for antibody or antigen and both work on the same principle‚ but the means of detecting specific binding is different. Radioimmunoassays are commonly used to measure the levels of hormones in blood and tissue fluids‚ while ELISA assays are frequently used in viral diagnostics. For both these methods one needs a pure preparation of a known antigen or antibody‚ or both‚ in order to standardize the assay. This assay will be described with a sample of pure antibody‚ but the principle
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Rh and Kell phenotyping (antigen typing) using the Diamed Gel Card system. Direct Coombs Test (DCT) using the conventional tube system. Direct Coombs Test (DCT) using the Diamed Gel Card system. Antibody Identifications (IAT) technique using the conventional tube system. Antibody Identifications (ETC) technique using the Diamed Gel Card system. Name: S. Ward Date: 8/11/2012 Introduction: The objective of this practical is to perform some pretransfusion tests‚ using various
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1. Explain why antibodies allow scientists to target and identify specific disease agents. The primary antibodies attach to the antigens that have the same receptors and the secondary antibodies do the same with the primary antibodies. 2. Why is the secondary antibody used in an ELISA test conjugated with an enzyme? What happens when this enzyme meets up with its substrate? The enzyme represents the weapon that the secondary antibodies (police) utilize to kill the antigen (foreign object)
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VIDAS Vitek Immuno Diagnostic Assay System VIDAS principle • Based on specific antibody and antigen reaction • Antigen = pathogen or its components in the sample See notes VIDAS substrate • One of the antibodies used has an enzyme covalently linked to it ⇒antibody-enzyme conjugate Colored product or light antibodyenzyme conjugate • Antigen detected by enzyme assay – Substrate added is converted to a colored or fluorescent product antigen Components of VIDAS System •
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by “memory” as a characteristic of the immune response. B. How does our immune response distinguish between our own cells and foreign invaders? C. What are lymphocytes and where are they produced? D. Distinguish between an antigen and an antibody. E. Why is the term “immune system” a
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does this mean for the rest of your results? A negative results with a positive control indicates a “false negative” and your results are invalidated. Direct Fluorescent Antibody Technique 4. Why is this technique a direct method? It is considered a direct method because it involves conjugation of an antibody with fluorescent dye. 5. What is an elementary body? An elementary body is an infectious particle of any of several microorganisms. 6. How do elementary bodies look under
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cases‚ the host survives and regains its health. This is because of the presence of antibody and lymphocyte in the blood of an individual that neutralize and attack foreign objects that can cause certain diseases like cancer. In order for us to produce large amounts of antibodies and lymphocytes to suppress foreign objects; we must have an enhanced immune system. The researcher investigated on how to increase the antibody and lymphocyte count to have protection against infectious diseases. Agaricus
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RHOgam! RHOgam refers to Rho immunoglobulin. RHOgam is an injectable blood product used to protect an Rh-positive fetus from antibodies produced by its Rh-negative mother. The injections are given at about 28 weeks in the pregnancy and immediately after delivery. With these injections‚ Rh-negative women can have safe Rh-positive pregnancies. Rh is short for Rhesus factor. Rhesus factor is a type of antigen present on the surface of red blood cells. If you have this particular antigen‚ you
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and histamine monocytes a large phagocyte lymphocytes antibody production Exercise 2: Blood Typing Questions _ A. Describe how the ABO blood typing system works. It is a system of classifying blood based on the presence of protein on the surface of the plasma membrane on the RBC. The protein is called antigen which is accompanied by antibody. B. Why is it important for everyone to know their own blood type? Because every antibody will react to a different RBC with different antigen causing
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