The Urea test was used to test both Unknown Gram-positive and Unknown Gram-negative bacteria. One colony of bacteria was used from the Unknown 13A plate and Unknown 13B plate in order to inoculate the Urea Slant. After the slant was inoculated‚ the cultures were placed in an incubator at 35ºC for Gram-positive bacteria‚ and 37ºC for Gram-negative bacteria for a total of 48 hours. If the Urea test were positive‚ the color of the slant would have changed to a hot pink color‚ which indicated the bacteria’s
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this experiment is to determine the unknown component of Panacetin. Theory: Panacetin is an analgesic (pain reducing) and antipyretic (fever reducing) drug that is sold in drug stores. However‚ there is a suspicion that this bottle may be counterfeit‚ not containing the chemicals that it should. Panacetin should be made up of about 50 percent of the unknown component that we previously separated out of Panacetin for testing. We suspect that this unknown compound is either acetanilide or phenacetin
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Maura Gongora 12th December‚ 2014 MCB 3020L- Section 018 Unknown #16 Abstract This report contains the background information on gram positive and gram negative bacteria‚ which will aid in understanding the use of specific laboratory experiments to distinguish between the two types of bacteria. Included are the materials and methods used to identify the gram positive and gram negative bacteria and methods which also differentiate between microbes of each group. The implications of the methods of
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Being able to identify unknowns is important in chemistry because it allows chemists to identify what chemicals they are working with if they have been improperly labeled or if they are trying to determine substances in a compound. Molecular weight and pKa are two properties that help to identify unknown compounds. To find the pKa of an unknown‚ pH meters are used during titrations to measure the potential difference in a solution by measure the difference of hydrogen-ion activity in a solution and
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products have become safer and less harmful to our environment and ourselves. Materials Various indicators ● Bro blue ● Phe red ● Met red ● Methyl blue ● Met orange ● Methyl yellow Various unknowns Watch trays Procedures 1. Take 6 indicators that are all different colors. 2. Take the 4 unknowns numbered 14 3. Take a watch tray and wash it down before using it. 4. Put the indicator that is numbered 1in 6 slots on the watch tray. (Only put in one drop) 5. Take each indicator and put it in 1 slot each
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the Unknown substances are bases or acids. Materials: goggles aprons paper for data table graduated cylinder unknown I substance beakers/test tubes stirring rod litmus paper pH indicator paper pH color chart phenolphthalein scoopula eye-dropper digital pH meter magnesium ribbon calculator unknown II substance forceps sodium bicarbonate Procedures: Test the pH of the Unknown substances with litmus paper and pH indicator paper and match to color chart Test the Unknowns with
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Abstract Chemically active extraction was used to separate two unknown compounds from each other and to determine what compound class each compound belongs. The solid unknown was purified by recrystallization and its melting point range was determined to be 109-111°C. The liquid unknown was purified by simple distillation and its boiling point was determined to be 95°C. An IR spectrum was collected for each of the purified compounds; the solid was determined to be 3-toluic acid and the liquid was
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is to find out the density of the unknown solid object by determining the mass and the volume through the experiment. To do this we will be get the mass of the object and determine the volume of the object by placing it into a liquid. Then we will divide mass by volume and get its density. PROCEDURE: 1) Obtain a solid metal 2) Weigh the dry sample carefully to the nearest 0.0001 grams on the top loading balance. 3) Determine the volume of your unknown solid sample by liquid displacement
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molecular formula were given for 3 unknowns. An effort was made to conclusively identify these unknown samples using only the spectroscopy data‚ specifically 1H-NMR‚ 13C-NMR‚ and IR spectroscopy. Although mass spectrometry was given for the samples as well‚ its use was suggested only for confirmation‚ not identification‚ of the unknowns. Mass spectrometry was not included in the identification analysis of the compounds‚ however was used for confirmation of unknown #46. The spectroscopic data was
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Identification and Separation of Dyes by TLC Pre-lab Properties: Ethyl acetate‚ ethanol‚ silica‚ azobenzene‚ azulene‚ 4-(p-nitrophenylazo) resorcinol‚ methyl red‚ bromocresol green (solubilities in water and ethanol) Purpose: To identify compounds from an unknown mixture using TLC Up to 100% of missed points can be recovered from this lab Watch the video: http://www.youtube.com/watch?v=e99nsCAsJrw (MIT) TLC plates are near the main hood DO NOT BREAK CAPILLARY TUBES Keep spots small and concentrated (video)
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