values of seven known color dyes and three unknown samples were observed in order to compare results and discover the hidden identities of the three samples. Three solvents‚ H2O‚ Isopropyl alcohol‚ and a 2% NaCl solution‚ were introduced to the dotted chromatography strips to show three different ratios for each dye on account of polarities. We found that all of the Unknown samples contained more than one pigment‚ sample one yielded Red #3 and Blue #1. Unknown sample two also revealed the same Blue #1
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drawn to the unknown. Both stories are about young boys and both have a female impact on them‚ both boys are drawn to new places unknown to them and in the end‚ both come out with disappointment because of shattered promises about the unknown. Firstly‚ in life‚ males and females are often fascinated by each other‚ because of the differences in their nature. Males are drawn to females because of their differences and vice versa. In this sense‚ to each other‚ the genders are the unknowns. In James
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point of a second content in the mixture and so on. By simple microscale distillation‚ we purified an unknown organic compound and then identified the unknown organic compound by using the boiling point and density measurements. Procedure/Results 5 mL of the unknown impure organic A was collected in an Erlenmeyer flask. A simple distillation apparatus was assembled and 3 mL of the unknown impure organic A was transferred into the distillation flask along with 3 boiling chips. A thermometer
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experiment is to determine the [NaOH] of a solution by titrating it with standard HCl solution‚ to neutralize a known mass of an unknown acid using the NaOH solution as a standard‚ to determine the moles of NaOH required to neutralize the unknown acid‚ and to calculate the molecular mass of the unknown acid. Procedure: Part A: Standarized 0.10M HCl solution and unknown NaOH solution were poured into two beakers. The burets were then filled with the solution and put into the flask. Water was
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identify an unknown analgesic tablet using Thin Layer Chromatography (TLC) analysis. The procedure involved preparing TLC plates‚ solubilizing standard analgesics (ibuprofen‚ aspirin‚ acetaminophen‚ caffeine) and the unknown in isopropyl alcohol (IPA)‚ and developing the plates in acetone. Rf values were calculated for each compound. The unknown tablet showed an Rf value of 0.584‚ closely matching that of caffeine (0.600)‚ suggesting similar polarity. The findings indicated that the unknown tablet likely
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Determining the Concentration of an Unknown Protein Solution with the use of the Bradford Method Paul Alcala‚ Andrea Basa‚ Melissa Caagbay‚ Frances Cayomba Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The Bradford method used to determine the protein content of a certain solution (Menguito‚ 2010) and involves the acidic Coomassie Brilliant Blue G-250 as a coloring reagent. [1] The dye is originally pinkish-brown in color when it is in its acidic state
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1310 Instructor: Dr. Gao Laboratory Assistant: Lauren February 7‚ 2015 Abstract: The purpose of this experiment is to determine the molar mass of an unknown solid. The freezing point of a solvent (Cyclohexane) was determined‚ and compare to the freezing point of Cyclohexane with the addition of two different concentration of unknown solid. The pure substance of Cyclohexane has defined physical properties. However‚ when a solute is added these properties are determined by the amount of solute
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The purpose of this lab is to measure urinary glucose (a known and unknown sample) using two quantitative tests: colorimetric strip test and o-toluidine test. Urinary glucose is the main focus for this lab due to its involvement with diabetes‚ a disease defined by the abnormal regulation of insulin. The most effective method for monitoring glucose is through blood glucose compared to urinary glucose. It has been discovered that urinary glucose is not a good measure of changes in blood glucose because:
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used to dissolve all samples used to compare unknown in TLC Silica Gel Plates- TLC plates. Stationary phase Elemental Iodine- used to accentuate the spots on the TLC plates so we can measure Rf. Asprin(Acetylsalicylic acid)- First compound used on TLC plate to compare the unknown. Salicylamide(o-hydroxybenzamide)-Second compound used on TLC plate to compare unknown. Caffine(1‚3‚7-Trimethylxanthine)-Third compound used on TLC plates to compare unknown. Acetone- Solvent A used in paper chromatography
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The objective of this lab was to identify an unknown compound by determining the melting point range using fast and slow ramps. Also‚ to determine the eutectic point of Urea and Cinnamic Acid. An unknown compound that was numbered 8 was chosen. A small amount of the unknown compound was placed in a weighing paper using a spatula. The weighing paper was folded in half twice and then was grinded using a spatula. After the unknown compound was grinded about 1-2mm was added into a capillary tube. A
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