Title: Purification of Green Fluorescent Protein Introduction: Transformation is used to introduce a gene coding for a foreign protein into bacteria. Hydrophobic Interaction Chromatography (HIC) is used to purify the foreign protein. Protein gel electrophoresis is used to check and analyze the pure protein. Research scientists use Green Fluorescent Protein (GFP) as a master or tag to learn about the biology of individual cells and multicultural organisms. This lab introduces a rapid method
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keeping the bacteria’s contents intact. The Cell Membrane: Is a phospholipid bilayer that completely surrounds a bacterial cell. Cell membrane acts as a highly selective barrier. This barrier prevents materials from diffusing into and out of the cell. This allows the cell to take up chemicals and nutrients needed for survival while keeping the cell components separated from the environment. The fluid and all its dissolved or suspended particles that can be found within a bacterial cell are called the
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Written Video Reaction Papers: On the Course Schedule you will see several fascinating videos that focus on questions we are addressing in the course. For each unit you should choose one of these videos to view. For that video‚ you will write a formal reaction paper that will be due as listed on the Course Schedule. The assignments system will not accept papers after this period. 1. ✓Do use essay form. 2. ✓Don’t include extra space between paragraphs -- simply hit the return key one time at
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Three-Dimensional Structure of Proteins Rotation around the -Carbon in a Polypeptide A Sterically Nonallowed Conformation The Helix and Pleated Sheet Conformationally allowable structures where backbone is optimally Hbonded (linear Hbonds). Helix (3.613 Helix): •3.6 residues/turn •Rise = 0.15 nm/ residue •13-atom hydrogenbonded loop Linus Pauling and Robert Corey‚ 1950 Pleated Sheet: •Anti-parallel or parallel •2.0 residues/”turn” •0.34 nm/residue (anti-parallel) or 0.32 nm/residue
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Understanding the Functions of Proteins and DNA Ingrid Waldron‚ Biology Department‚ University of Pennsylvania‚ 2012 These key concepts and activities are suggested as part of an introductory unit on biological molecules (or as an introduction to a unit on molecular biology). The overarching goal is to help students understand that proteins and DNA are not just abstract concepts in biology textbooks but rather crucial components of our bodies that affect familiar functions and characteristics
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Biochemistry: Biological Molecules Pg. 2 – 7 Carbohydrates Pg. 8 – 12 Proteins Pg. 13 – Lipids Carbohydrates. L.O. 1.1 and 1.2 Carbohydrates are sugars and they are the primary source of energy for our vital functions such as breathing and sleeping. There are two types of carbohydrates‚ simple and complicated. Monosaccharides‚ disaccharides and polysaccharides. L. O. 1.2 Carbohydrates are split into different classifications depending on how many molecules they have. Monosaccharide: one sugar
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17 May 2013 Are Protein Shakes Good For You? According to Flex bodybuilding magazine‚ protein shake is the number one selling bodybuilding supplement. Protein powders have been around for years‚ but they are currently gaining popularity and becoming more widely used for their health benefits. In fact protein is one of three food components we require in large quantities. Others are carbohydrates and fats. Your body uses proteins in your diet to build new cells‚ maintain tissues‚ and synthesize
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For my bookworm project I read the book called A Walk Across America by Peter Jenkins. In 1973 Peter Jenkins sets off to discover America by hiking from New York to the Gulf of Mexico. Jenkins is part of a disillusioned generation fed up with the war in Vietnam‚ assassinations‚ social injustice‚ greedy corporations‚ and pollution. Recently divorced and newly graduated from Alfred College in New York State‚ he sets out on his quest with his dog Cooper‚ a large mixed Malamute. Hoping to find something
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lacked protein‚ and three samples containing proteins‚ and using a spectrophotometer we assessed the amount of light absorbed versus the light transmitted‚ based on the principles of the Beer-Lambert Law. The three proteins used included lysozyme‚ protamine sulfate‚ and bovine serum albumin‚ and the three non-protein samples contained either RNA‚ tyrosine‚ and glycylglycylglycine. Standard curves were created to exhibit the linear relationship between the concentration of solute (protein‚ non-protein)
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Starch‚ one variable may be observed. 2. Biuret reagent will turn your skin brownish-purple. Explain why this occurs. Biuret reagent will turn your skin a brownish-purple because Iodine detects protein in a substance by changing the color to a brown or purple shade‚ and since your skin contains protein it will also change color. 3. When greasy food is spilled on clothing‚ why is it difficult to clean with water alone? Water cannot remove grease water is polar and grease is non-polar. They are
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