MULTI-STATE INFECTION Multi- State Escherichia coli O26 Infection Linked to Raw Clover Sprouts Escherichia coli are gram- negative bacteria that are normally found within the gut of warm bloodied organisms. There are several strains of E. coli that exist as part of the normal flora of the human digestive system. They prevent harmful bacteria from establishing themselves in the intestines‚ and they also aide in the production of vitamin K2. Most E. coli strains are not harmful to humans‚ except
Premium Escherichia coli Bacteria
Abstract:Conjugation is a natural occurring process that involves the transfer of DNA from one cell into another through a physical connection between the cells. In the following experiment‚ two strains of Escherichia coli bacterial cells (donor F’lac+strs and recipient F-lac-strr) underwent conjugation to produce a transconjugant strain (F’lac+strr). MAC plates and streptomycin were utilized to determine if conjugation had occurred. When plated‚ the donor colonies appeared red and the recipient
Premium Plasmid Bacteria Molecular biology
price‚ and high degree of reduction have made glycerol a highly attractive and exploited carbon source for the production of fuels and reduced chemicals. Here we report the quantitative analysis of the fermentative metabolism of glycerol in Escherichia coli through the use of kinetic modeling and metabolic control analysis (MCA) to gain a better understanding of glycerol fermentation and identify key targets for genetic manipulation that could enhance product synthesis. The kinetics of glycerol fermentation
Premium Metabolism Glycolysis Adenosine triphosphate
Material and methods Hosts ‚ plasmids and chemicals E. coli TOP10 strain was used for cloning and proliferation. E.coli BL21 DE3 was used as expression host cell. The pET 28a(+) plasmid was employed for gene expression. All chemicals were obtained from Merck Company (Germany). Codon optimization and gene synthesis Sequence encoding V-domain was obtained from Swiss-port‚ Uniprot KB and National Center for Biotechnology Information (NCBI) databases. 6x His-tag sequence was placed at N-terminal
Premium DNA Gene Molecular biology
commonly used techniques to test motility? C. Why are semi-solid media used to test for motility? D. Why might it be difficult to observe motility in a wet mount? E. Why is it important to use a needle rather than an inoculating loop when inoculating a motility tube? F. For which of the organisms on the wet mount‚ if any‚ were you able to observe motility? G. For which of the organisms in the motility medium tubes‚ if any‚ were you able to observe motility? Questions A. Define the following
Premium Bacteria Eukaryote Metabolism
Jennifer Hauss March 4‚ 2015 Bacterial Transformation Lab Report Introduction In this lab‚ the goal was to transform the bacteria e-coli to glow in the dark (or under a black light). Four plates were set up with agar in them for the bacteria to feed on and grow. Changes were then made to the bacteria. One plate was the control plate‚ having only the LB or agar for the bacteria and negative pGLO‚ which is the liquid not containing the plasmid. This is the plate that was compared with the three
Premium Bacteria Escherichia coli DNA
Commission‚ to produce legal documents related to the “isolated” outbreak‚ Although uncommon the FDA and prosecutors are working together to try to bring increased attention and punishment to companies found in outbreak violations.During the investigation E. Coli outbreaks raged through Chipotle stores across America. In total 57 consumers in twelve states were affected were hit with some strains of norovirus. Due to the investigations and outbreaks Chipotle
Premium United States Genetically modified organism Genetically modified food
Optimal Growth Conditions and Generation Time for E. coli Introduction The goals of these exercises were to determine the optimal growing condition and the generation time of E. coli. Materials and Methods Please refer to the lab manual for a complete list of materials. To determine the optimal growth condition for E. coli‚ we had to test the environmental factors that could affect the growth. We tested the relative growth of E. coli in the presence and in the absence of oxygen. The results
Free Bacterial growth Temperature Celsius
BIO Microscopy‚ Mitosis‚ and Motility Lab Discussion Questions In the Microscopy‚ Mitosis‚ and Motility lab‚ the class used the Compound Microscope. The Compound Microscope helped examine the prokaryotic and eukaryotic cells‚ observe the different stages of mitosis‚ and compare modes of motility used by protists. The light compound microscope works with the aid of lenses such as the eyepiece. These lenses help keep the focus of the light on the eye. The fine and course adjustments also assist with
Premium Optics Microscope Lens
The effects of black tea on the growth of E. coli bacteria cultures Kierstin Barker‚ Melissa Bischak‚ Jackie Tyszkiewicz‚ Errin Enany September 25‚ 2012 Abstract: This study was carried out in order to investigate whether black tea has antimicrobial properties as stated in Steven Johnson’s The Ghost Map. If tea does have antimicrobial properties‚ then it could aid in warding off waterborne diseases. We believed that if black tea is steeped in boiled water‚ then the amount of bacteria exposed
Premium Tea Green tea Antimicrobial