order from 10^5‚ 10^6‚ 10^7‚ then have the blank dilutions ready in order from 10^-4‚ 10^-5‚ 10^-6 2. The pipettes was used and pipette 0.5 ml of the phage suspension to the 10^4 3. The next step was pipette 0.5 ml of dilution suspension from 10^-4 and added it to the 10^-5 4. 0.5 ml of dilution suspension was pipette from 10^-5 and add it to the 10^-6 5. We then took 0.1 ml from the 10^-6 and add it into the agar. 6. After adding the dilution into the agar‚ we added seven drops of E. coli to
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rate is used against the inverse of the temperature kelvin’s the Arrhenius equation is used to calculate the activation energy. The range in orange is between 16.5 - 37oC and the activation energy is calculated to be 9332kcal/mol. Discussion: The dilution made for all the assays was 8x which falls between an absorbance of 0.5-1.0 when measured at 405nm. On Figure. 1‚ it shows the variation of rate as a function of the weight of alkaline phosphatase. In the graph‚ the rate
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Foreign Ownership: When Hosts Change the Rules The forces of globalization‚ which have led to more liberal trade regimes‚ new technologies‚ managerial innovations and the creation of new competitive pressures are all exerting a worldwide strong impact. These global developments gave idea to the policy makers of every host country to balance the economic considerations‚ national sovereignty goals and other policy objectives. That is the reason why the governments all over the world are vying
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Abstract: During the lab a better agent for absorbing Procion Red Dye was trying to be found. Using the different dilutions of a stock solution and a spectrophotometer an Absorbance vs. Concentration graph was created and using the trendline from that graph it was determined which agent‚ zeolite‚ magnetic zeolite‚ or charcoal were better at absorbing the dye. From the results collected it was found that charcoal was the best at absorbing the dye‚ when moles of dye per gram of agent used were calculated
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TITLE OF EXPERIMENT 2 : DILUTION 2.1: OBJECTIVES Upon completion of this experiment‚ students should be able to: 1. determine the concentration of coloured solution such as FeCl3 by using dilution and colour differentiating (colorimetric) techniques. 2.2: INTRODUCTION Concentration can be expressed in many different ways such as percentage volume and percentage weight etc. In laboratory‚ normally concentrations were expressed as molarity and normality. Molarity is the
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Ethanol Production From Food Waste A PROJECT REPORT Submitted in partial fulfillment of the requirements for the Award of the Degree of Bachelor of Technology (Biotechnology) Under the Guidance of Dr. S.M. Bhatt (Associate Professor) Department of Biosciences By Abhishek Agarwal Registration No. 10809065 Roll No. RB18B2A07 Department of Biotechnology Engineering Lovely Professional University Phagwara –144401 November 2011 CERTIFICATE This is to certify that Abhishek
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the effect of enzyme concentration and substrate concentration on the reaction rate‚ we performed a serial dilution of enzymes to get test tubes with full‚ one-half‚ one-fourth‚ and one-eighth concentrations. We then used a spectrophotometer to get the absorbance reading over time‚ and we used these readings to determine the reaction rate. We did the same thing with a serial dilution for substrate concentration‚ and we found the reaction rate for these as well. We found that as enzyme concentration
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DEFINITION ANAEROBIC POND Anaerobic ponds are deep treatment ponds that exclude oxygen and encourage the growth of bacteria‚ which break down the effluent. It is in the anaerobic pond that the effluent begins breaking down in the absence of oxygen "anaerobically". The anaerobic pond acts like an uncovered septic tank. Anaerobic bacteria break down the organic matter in the effluent‚ releasing methane and carbon dioxide. Sludge is deposited on the bottom and a crust forms on the surface as show in
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Effect of Osmotic Stress and Temperature on Microbial Growth BIO 3400-002L – Microbiology Lab 1 Effect of Osmotic Stress and Temperature on Microbial Growth Luiz Felipe Isidoro ABSTRACT Evolution allowed primitive forms of life to develop proteins and enzymes that made it possible for life to evolve under environments with hostile conditions‚ such as high salt and heat. More specifically‚ some bacteria selected genes that code for peptides with stronger intermolecular forces‚ coping
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this diameter was increased‚ then the slope of the standard curve would react by decreasing. These values are close‚ however‚ not the same. This could be due to a slightly less than perfect R2 value or pipette delivery variation when creating the dilution solutions.
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