Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
Free Bacteria Staining Gram staining
absorbance values for the unknown is 0.251 (equation 6). The standard deviation is 0.0222 (equation 7). Using the Beer’s Law plot and the average absorbance value‚ the concentration of the Fe solution after dilution is 1.29 µg/ mL (equation 8). The concentration of Fe solution before dilution is 0.000108 mol/L (equation 9). The mass of Fe in the original vitamin tablet is 1.61mg (equation 10). From the standard deviations of the slope and intercept of the calibration plot‚ and the average deviation
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a trademark infringement‚ or rather a trademark dilution for using the prefix “Mc”‚ in Federal District Court for Court in Maryland‚ at the original jurisdiction level‚ on September 24‚ 1988. “Modern trademark law in the United States stems from the Federal Trademark Act of 1946‚ commonly called the Lanham Act.” (Cooter & Ulen) It is discussed in Chapter 5 in the text book. The concise rule of law of this case can be found in Illinois Anti-Dilution Act‚ Ill.Rev.Stat. Ch. 140‚ § 22. In 1964‚ the United
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a sterile stomacher bag and shaken for two minutes with 225 ml of peptone water to obtain the food mixture. Using separate sterile pipets‚ decimal dilutions of 10-2‚ 10-3‚ 10-4‚ 10-5 will be prepared and others as appropriate‚ of food homogenate by transferring 10 ml of previous dilution to 90 ml of diluent. Using a micropipette‚ add 10 μl of dilution quickly and smoothly without interruption‚ to the v-shaped groove on each side of the hemacytometer. Hemocytometer was cover using coverslip counted
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Using Kanamycin Resistance Bacteria to find the Sources of Contamination of Three Chicken Farms Introduction: Kanamycin is a common antibacterial that interferes with bacterial growth‚ by inhibiting protein synthesis‚ and causing the mistranslation of mRNA. Kanamycin is commonly used in chicken feed to keep harmful bacteria from getting into the eggs and producing healthier chickens. Recently reports of severe gastroenteritis have been linked to eating raw or undercooked eggs. This has led to
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room temperature shaker. In the last part‚ the number of colonies grew in each LB plate of different diluted blank concentrations were determined. First‚ 0.1ml of DH5α culture was added into a sterile 9.9ml dilution blank. Next‚ 0.1ml of this solution was added to another sterile 9.9ml dilution
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Question 3 Using any peripheral nerve bundle as a model‚ describe using figures (a) the general anatomical structure with key constituents identified and (b) the heterogeneity in the electrical conductivities of the different constituents. For this question‚ I will be using the sciatic nerve as my model. The sciatic nerve consists of several main structures as can be seen in Figures 5 below. Figure 5: Cross section of the sciatic nerve. The inset in the top image shows individual fibers wrapped
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substrate? The enzyme catalyzes the oxidation of the substrate and turns the solution blue. 3. Disease samples from two patients are collected and subjected to serial dilutions before running an ELISA. What does it mean if a disease can be detected in samples from one person at a dilution of 1/5 and in another patient at a dilution of 1/100? One patient has a higher concentration of antigens. 4. Describe a situation that illustrates why it is a good idea to complete the ELISA assay in triplicate
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occupational health. (2) (b) Outline the personal hygiene practices that should be followed to reduce the risk of ingestion of a hazardous substance. (6) 3 (a) Explain the meaning of the term `dilution ventilation’. (2) (b) Outline the circumstances in which the use of dilution ventilation may be appropriate. (6) 7 In relation to substances used at work: (i) explain the meaning of the term `toxic’ (2) (ii) describe‚ using an example in EACH case‚ the differences
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solution I2KI solution 1% starch solution 1% amylase solution Methods The lab group prepared amylase dilution in the first set of test tubes (1-5). 5mL amylase was added to a test tube already containing 5mL distilled water creating the first tube to have .5% amylase. Next 5mL of amylase from test tube 1 was added to the 5mL of distilled water in test tube 2‚ giving test tube 2 a dilution of .25%. This was
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