An experiment to determine percentage of cell viability by using serial dilutions and a haemocytometer Aim The aim of this experiment was to determine accuracy of the cell count and how valid the result of the experiment will be. Materials and Methods Refer to Scientific and Laboratory skills practical booklet. (Pg. 10-Pg. 12) Results Table one: Raw data (Viable cells) Tube Counts (4x4 grid) Counts (4x4 grid) Counts (4x4 grid) Counts (4x4 grid) Average Count A 34 31
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Running head: Beer’s Law And Calorimetry Beer’s Law And Calorimetry Adriane Bellard Ocean County College Beer’s Law is also referred to as the Beer- Lambert law or the Bouguer- Beer Law. The principle is based on an electromagnetic radiation that is passed through a sample‚ wavelength is detected by the sample. As a result strength of transmitted light is gradually reduced. The measurement of the reduced strength of radiation is supported by the spectrophotometer. Based on Beer’s
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Serial Dilution Activity Many applications require the determination of microbial numbers. Those applications can be either clinical or in a research setting. Clinical applications include determination of antibiotic efficacy and as well as therapy. Research applications include determination of the effectiveness of antimicrobial chemicals‚ radiation‚ etc. The viable count is most common or standard method used to quantitate bacteria. With this method only microbes that are alive and able to reproduce
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Professor Rotibi ABSTRACT: Accurate evaluation of bacterial colonization as a predictive index for alfalfa sprouts has relied on a quantitative culture technique that provides exact colony counts per gram of tissue by culture of five serial dilutions of the alfalfa water. In this study 1 package of alfalfa sprouts were cultured by a semi-quantitative technique that enumerated the number of gram-negative enteric organism in 1 ml of alfalfa water. Exact colony counts from the experiment were available
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of getting viable counts are available i. Spread plate method. ii. Most probable number (MPN) method. (I) SPREAD PLATE METHOD (LAWN CULTURE) MATERIALS: Dilution series prepared in (A) Sterile 1ml pipette 6 nutrient agar (NA) plates 1 glass hockey stick 1 beaker of alcohol PROCEDURE All steps should be done using aseptic technique The culture labeled 10-8 was gently mixed. 0.1ml of this dilution was aseptically transferred onto the center of a NA plate. Bend end of the glass spreader
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latter involves serial dilution and spread plating of bacteria on agar plates. Materials required per pair • One 10 ml liquid culture of Escherichia coli BL21 (see prior preparation) • A sample of Yakult (approximately 5 ml) • Marker pens to label plates & bottles • Sterile plastic loops for streaking bacteria (up to 20 per pair) • Sterile plastic spreaders for spreading bacteria (4 per pair) • Sterile universals/bijoux bottles (8 per pair) for serial dilutions • 10 ml sterile phosphate
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spectrum to determine the concentration of light absorbing molecules in a solution. (p.59) In this particular lab‚ our mission was to determine the protein concentration and the standard curve of the unknown sample of BSA. This‚ by preparing five dilutions of the unknown solution of BSA together with other known concentrations‚ and then experimenting by observing how the concentrations were passed through the spectrophotometer. The outcome resolved in the absorption levels being decreased‚ and this
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A merger is a combination of two companies where one corporation is completely absorbed by another corporation. The less important company loses its identity and becomes part of the more important corporation‚ which retains its identity. It may involve absorption or consolidation. Merger is also defined as amalgamation. Merger is the fusion of two or more existing companies. All assets‚ liabilities and the stock of one company stand transferred to Transferee Company in consideration of payment in
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DCP & CE Title The effect of the different dilutions of yeast cell suspension on the number of yeast cells per cm3 that counted using haemocytometer under microscope. Aim To investigate the effect of the different dilutions of yeast cell suspension on the number of yeast cells per cm3 that counted using haemocytometer under microscope. Research Question Do the different dilutions of yeast cell suspension affect the number of yeast cells per cm3 that counted using haemocytometer under
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growth of yeast is measured by using spectrophotometer and hemocytometer.We learnt how specthophotometer and hemocytometer use and also we learnt qualifications of hemocytometer and spectrophotometer.Serial dilution was used for this experiment and it was very important.Because of the serial dilution‚we measured the number of yeast cells. The graph of growth curve was drawn and bacterial life cycle was understood with the graph.The purpose of the experiment was to calculate and draw bacterial growth
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