allowed the students to perform the plate count technique by serial dilution and two common methods‚ spread plate and pour plate to determine the colony forming unit (CFU) of yeasts A ten-fold dilution is used in this experiment‚ the sample is diluted until it reached the 10-9 dilution. Plating for spread plate started from 10-5‚ 10-6‚ 10-7 and 10-8 dilution while for pour plate‚ it started from 10-6‚ 10-7‚ 10-8 and 10-9 dilution. Having incubated inverted at room temperature (25oC) for 2 days‚
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Pre-Lab 1 Assignment Quantitative Analysis of Biomolecules Biochemical analysis involves the characterization of biomolecules within a sample using appropriate laboratory techniques. There are two principal approaches: 1. Qualitative analysis – where a sample is analyzed to determine whether a biomolecule is present or absent. As an example‚ a blood sample might be analyzed for a specific antibody or a bacterial cell might be probed for a nucleic acid sequence. 2. Quantitative analysis
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(2mg/mL=2000 ug/mL)‚ we prepared the concentration of the protein standard by carrying out serial dilution at 2x‚ 5x‚ 10x‚ 20x and 40x. The 2x dilution was made by mixing 250 uL of BSA and 250 of the homogenizing buffer. The solution was then vortexed for 10 seconds. A similar procedure with different concentration of BSA and stock solution was carried out to find the other diluted concentrations. After the dilutions were made‚ 200 uL of the BSA concentration from the diluted tubes (2x‚ 5x etc.) were transferred
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etc.) Remember to cite information and put responses in your own words to avoid plagiarism (and loss of credit on the assignment). 1. Refer to step 2 in the protocol for lab 6. Calculate the concentration of the sample in tubes 2-5 for the serial dilutions in the chart below. You may wish to refer to http://www.wellesley.edu/Biology/Concepts/Html/standardcurve.html Tube number Water (ml) Blue Solution (ml) Total Final volume (mL) Concentration (mg/ml) 1 0 5.0 2.5 1 mg/ml 2 2.5 2
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CONCENTRATION AND DILUTION Physiology 1‚ Las Positas College Name: In science‚ concentration is a measure of the number of particles (solutes) in a given volume. If one room has 100 people in it‚ and a room of equal size has 50 people‚ one can say that the concentration of people in one room is twice that of the other. Quite simple‚ isn’t it? On a molecular level‚ consider whether you put one lump or two of sugar‚ or no sugar at all‚ in your coffee. If you use two lumps‚ you prefer twice
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OD was surveyed at 595. The UK protein in µg was assessed by taken the equation y=0.0259x+.0511 (Fig. 1) and solving for x where y is the OD. Calculation of UK Samp ID | µl of UK Assayed | OD @ 595 | µg UK protein | µg UK/µl(Diluted Sample) | Dilution Factor | UK Conc in µg/µl | 1:50 | 10 | .215 | 6.33 | .633 | 50 | 31.65 | 1:100 | 10 | .092 | 1.58 | .158 | 100 | 15.80 | 1:250 | 10 | .075 | 0.92 | .092 | 250 | 23.00 |
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1. Introduction: The goal of this lab was to demonstrate the microbiology technique of serial dilutions and how they can effectively be used in experiments. E. coli cells were exposed to UV light for various amounts of time in order to asses the effect on growth and thereby mutations since this cell was modified to not have photolyse no uvr genes for DNA repair and thus can only use the SOS response. Each group then diluted the cells accordingly based on their UV exposure time and counted the cells
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economic value of a famous mark is a requisite for relief under the 1996 Federal Trademark Dilution Act (FTDA)”. 1 Contrary to lower courts’ holdings‚ the Supreme Court stated in a unanimous decision that it is not enough to claim that another trademark is merely likely to dilute a famous trademark‚ and the standard for dilution requires a plaintiff to prove that another trademark actually causes dilution of the distinctive quality of a famous trademark. In my opinion‚ the above-mentioned holding
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First of all “A serial dilution is a series of sequential dilutions used to reduce a dense culture of cells to a more usable concentration . Each dilution will reduce the concentration of bacteria by a specific amount” .( Study . com / Serial Dilution ) A serial dilution is any dilution were the concentration decrease by the same quantity in each successive step . The purpose of serial Dilution is to reduce the concentration of cells
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whether or not the fact that Gabby’s surname is Rally gives her the right to use it any way she wishes. The fact that Gabby’s name is Rally does not give her the right to use Rally in her business. The Lanham Trademark act and the Federal Trademark Dilution Act of 1995 protect a company’s ownership rights to names‚ logo‚ or symbols that identify its products. In this case‚ Rally was identified by cars‚ now the name is also being identified for making pizza making it harder to distinguish between the
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