The samples are chosen as fasting (0) and 2 hour (2) for example B0 and B2 and can be used directly without dilution. But sample A2 needed to be diluted 1 + 2 with distilled water before use i.e. I ml of A2 was delivered into a clean LP4 tube by glass pipette followed by 2 ml of water. This diluted sample was used for the assay. Sample D was also prepared in the same way but without dilution. Duplicate tubes were prepared from 200 micro litres of all samples A to D.Standards were prepared from the
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1. The final yield of the cells can be determined by use of an automated cell counter or by use of a hemocytometer (Neubauer chamber). In our laboratory‚ we determine the number of purified cells in a Neubauer-improved‚ bright line chamber with V-slash with a depth of 0.1 mm and a counting area of 0.0025 mm2 from Marienfeld (Lauda Königshofen‚ Germany‚ PN # 0650030). 2. For determination of the final cell yield‚ mix a 10 µl aliquot of the purified cells (Figure 9D) with 10 µl of trypan blue solution
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Title: Acids‚ Bases & Buffers Objectives: 1. To understand the acid-base chemistry. 2. To prepare and evaluate a buffer system 3. To measure the buffering capacity of two types of isotonic drinks. Introduction: There are acid-forming‚ basic forming and neutral food‚ however the acid or alkaline properties of a food is unable to judge by the actual acidity of the food itself. For example‚ citrus fruits such as lemon are acidic‚ but they are alkaline-forming when we consume and digest it. Therefore
Free PH Buffer solution Acid dissociation constant
This essay will discuss the comparative benefits and costs of two forms of reproductions; parthenogenesis and sexual reproduction. To narrow the scope of the piece‚ the area of parthenogenesis is going to be largely limited to organisms that are obligately parthenogenetic. Parthenogenesis is the “production of an embryo from a female gamete without any genetic contribution from a male gamete” (Mittwoch‚ 1978). Sexual reproduction is the process of cross-fertilisation in which the genomes of two parents
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Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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and galactose‚ but its function diminishes with age resulting in a wide use of lactase supplements. This enzyme functions under specific conditions‚ so we investigated the effect different salt concentrations have on the enzyme activity. Serial dilutions were performed to prepare a lactase solution as well as twenty percent‚ fifteen percent‚ and five percent concentrations of NaCl. Three different treatments of ortho-nitro-phenyl-galactoside‚ lactase‚ and perspective salt concentrations were prepared
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Chemistry 12 Santa Monica College Determination of Kc for a Complex Ion Formation Objectives • • Find the value of the equilibrium constant for formation of FeSCN2+ by using the visible light absorption of the complex ion. Confirm the stoichiometry of the reaction. Background In the study of chemical reactions‚ chemistry students first study reactions that go to completion. Inherent in these familiar problems—such as calculation of theoretical yield‚ limiting reactant‚ and percent yield—is
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OSMOSIS Aim To determine the water potential of potato tuber cells. Background knowledge Osmosis is defined as the movement of water molecules from a region of higher water potential to a region of lower water potential through a partially permeable membrane. Osmosis is considered in terms of water potential and solute potential. Water potential is a measure of the kinetic energy of water molecules. Here‚ water molecules are constantly moving in a random fashion. Some of them collides with cell
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The plates were incubated for 48 hours and then observed for a zone of clearing where the cilantro juice drop was placed. Cilantro was found to not display antimicrobial activity against either bacterium in the experiment in any of the different dilutions. These results could have to do with the possible contamination of cilantro or the specific bacteria we chose to target. Additional studies of cilantro should be conducted on different types of bacteria in order to gain a better understanding of
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Laboratory Report 1 Title : Accurate Measurement of Mass and Volume Part A: The Formula of Hydrated Copper (II) Sulfate Aim: The objective of this experiment is to find out the accurate mass of a solid and to calculate the moles of an unknown. Materials: The materials used in this experiment are Hydrated Copper (II) Sulfate‚ weighing bottle‚ analytical balance‚ laboratory balance‚ casserole‚ spatula‚ and hotplate. Methods: First‚ approximate 1.0g of hydrated copper (II) sulfate was
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