absorbances using the Cary50 set at 250nm. The pre-dialysis elution pool was pooled together from fractions 17-18 and the flow-through pool was determined to be fractions 2-9. Figure 2: Bradford standard curve created by an assay of seven known dilutions of bovine serum albumin (BSA). The assays were done at 595nm using a
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Kimmi Dodia- Biomedical Science- 1019971 Lab partner- Kim Harcourt Lab report- Experiment 1: Microbial Genetics Abstract The objective of this experiment was to introduce the study of bacterial genetics in order to identify the potency of different mutagenic agents. Our primary aim was to demonstrate the different techniques needed to isolate biosynthetic auxotrophic mutants using chemical‚ physical and transposon mutagenesis. The second aim was to plan and execute an experiment designed
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References: 10 Isolation of Recombinant Taq Polymerase for PCR TABLE 1 Taq Sample Dilution Factor 10 x Absorbance at 620 nm (+ 0.0005) 0.881 1.145 0.951 0.449 0.429 0.391 Protein Concentration (corrected) 4.10 + 0.47 mg/mL
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Business Economics Case Analysis: “No Frills” Air Fares Distinguish between the demand curves for National Airlines‚ Eastern Airlines and the Airlines industry. The above analysis requires an understanding of: (i) Why is the demand curve downward sloping? (ii) Can price have the same effect on the demand between the firms and at industry level? (iii) What would be the effect of changes in income and other prices on the demand curve of a firm? (iv) Calculate the price elasticity of demand
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Determination of Trace Amount of Copper and Zinc in an Aqueous Solution by Atomic Absorbance Spectroscopy Abstract: The purpose of this experiment is to analyze a sample containing copper and zinc in trace amounts‚ where standard addition method was used and the instrument used is the atomic absorbance spectroscopy. The amount of copper obtained was 0.569 ppm ± 0.015 and for zinc we obtained 0.42 ppm ± 0.0027. Introduction: This method implies the excitation of particles in the solution sample
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The majority of the experimentation in this project relied on the idea that there is a relationship between concentration and absorbance of a solution. This is easy to understand if you can visualize the particles in the solution; and this is especially true if the particles in solution portray a distinct color. The more particles there are in the solution‚ the more they will obstruct the path of the light. This occurs the most at the analytical wavelength‚ where the most light is absorbed by
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Measurements Lab Name Length Measurements – Follow the Instructions in the Lab Manual and fill in your data in the tables provided. Data Table 1 – Length measurements |Object |Length (cm) |Length (mm) |Length (m) | |CD or DVD |12.00 |120.0 |.1200 | |Key
Free Density Water Volume
General Chemistry II Determining of the equilibrium constant for the formation of FeSCN2+ Introduction The objective of this experiment was to determine the equilibrium concentration and then determine Kc. A dilution calculation was formed to determine the concentration of SCN- and Fe(SCN)2+. Each cuvette was filled to the same volume and can be seen in table 1. Then the absorbances were recorded from each cuvette and can be seen in table 1. A Beer’s law plot was made from the data that was recorded
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time to adapt to these conditions‚ it is happening more drastically than slowly. By these ice caps melting‚ there are going to be many drastic changes to the environment in Antarctica‚ and all around the world. One of the main concerns is the dilution of the oceanic water. Once theses two ice sheets melt all the way‚ the ocean levels can raise up to 200 feet‚ which will put hundreds of countries underwater. In the past century‚ the sea level has rose four to ten inches in the past century. It
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An Le Foundation Biology lab BRADFORD PROTEIN DISCUSSION ESSAY The appearance of blue color showed the present of protein in the BSA dilutions. The more diluted the solution was‚ the less blue it was. The R2 value of the standard curve of BSA dilution was obtained to be 0.9972‚ which is close to 1. The closer to 1 the R2 value was‚ the more accurate the linear portion was. The error percentage of each unknown was large: 25.9% for skim milk‚ 95% for soy sauce‚ and 64.7% for egg white. The vast
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