Protein Concentration using BCA and Bradford Assays Introduction There are several ways to determine protein concentration for a sample such as The Lowry Method‚ Bradford assay‚ and Bicinchoninic Acid (BCA) just to name a few. Determining the protein concentration in your sample is an important step in any laboratory workflow that involves protein extraction and/or analysis (1). Based on the assay being used‚ different chemicals can alter the accuracy for the results. For example‚ in BCA‚ the reducing
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Effect of temperature and/or dilution on buffering capacity: Temperature Figure 3: Method for effect of temperature on buffering capacity Dilution Figure 4: Methods for effect of dilution on buffering capacity Table 1: Table of dilution Dilution ratios Volume of Phosphate solution (mL) Volume of tris buffer (mL) Volume of distilled water (mL) 1:10 2.0 2.0 18.0 1:50 0.4 0.4 19.6 1:100 0.2 0.2 19.8 Dilution 1:10 dilution Volume of buffer needed
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graph was taken to calculate the protein concentration‚ where corrected absorbance at 595nm was y and protein concentration was x. The protein concentration was divided by its respective dilution factor in order to account for how much the protein was put in. Figure 5 shows the protein concentration from each dilution factors for each stage enzyme that were calculated using the equation from figure 4. The protein concentration mostly decreased from stage 1 to stage 4‚ as expected from the
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beakers were used for this experiment. In one‚ a 20 mL of 1% hydrogen peroxide solution was made. In order to get this‚ the stock solution‚ which was 3%‚ was diluted using 10 mL of hydrogen peroxide and 20 mL of water. In the second beaker‚ a ¼ dilution of potato extract was made using 2 mL of potato extract and 6 mL of water. The third plastic beaker contained 8 mL of water. Using the first and second beaker the experimental assay was performed. Using forceps a small paper disk was dipped into
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OF THE ISSUES 1. Whether summary judgment in favor of Visa’s dilution claim was proper‚ when Visa’s famous trademark‚ “VISA‚” is not distinctive – 1 tem1506.doc indeed‚ is generic -- with respect to circumstances in which the English word "visa" is used in its immigration/travel sense and that is the sense in which JSL uses the word within its mark‚ “evisa.” 2. Whether summary judgment in favor of Visa’s dilution claim was proper under the U.S. Supreme Court’s recent case
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What is chromatography- Chromatography is the separation of mixture by passing it in solution or suspension or as vapor. It’s a technique for separating mixtures into the components this needs to happen in order to do the 4 things analyze‚identify‚purify‚quantify. Many scientist use this to do the 4 steps. When analyzing its used to examine the mixture and to find out the relation with one another. In purifying you need to seperate or take away and put it by itself for further study. In identifying
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concentrate A solid or liquid intended for dilution before use as a bait. CP Contact powder Rodenticidal or insecticidal formulation in powder form for direct application. Formerly known as tracking powder (TP). CS Capsule suspension A stable suspension of capsules in a fluid‚ normally intended for dilution with water before use. DC Dispersible concentrate A liquid homogeneous formulation to be applied as a solid dispersion after dilution in water. (Note: there are some formulations
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Harley−Prescott: Laboratory Exercises in Microbiology‚ Fifth Edition Front Matter Preface © The McGraw−Hill Companies‚ 2002 PREFACE Take interest‚ I implore you‚ in those sacred dwellings which one designates by the expressive term: laboratories. Demand that they be multiplied‚ that they be adorned. These are the temples of the future—temples of well-being and of happiness. There it is that humanity grows greater‚ stronger‚ better. Louis Pasteur (French chemist‚ founder of microbiology
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For experiments 1and 2‚ many different tests were conducted in order to investigate the different microbes found on the different types of turkey sampled. To begin‚ our group obtained 5 different forms of turkey that are encountered in everyday life: Inspirations deli turkey from Hannaford supermarkets‚ turkey bacon‚ turkey jerky from Whole Foods‚ Inspirations ground turkey from Hannaford‚ and a raw whole chunk slice of turkey breast form Whole Foods‚ these foods were considered experiment 1. Experiment
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pomegranate gatorade. Procedure: We created dilutions using stock solution(10mL stock/0 mL water‚ 8mL/2mL‚ 6mL/4mL‚4mL/6mL‚ 3mL/7mL‚ 2mL/8mL‚1mL/9mL‚ 0mL/0mL). Before we put each dilution in the SPEC 20‚ we put water in a cuvette and put it in the SPEC 20 so we could zero out the percent transmittance. We collected the percent transmittance of each dilution using the SPEC20 with a wavelength of 630nm. We calculated the molar concentration of each dilution using the formula M1V1=M2V2 We determined
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