and the Ag+-Zeolite/gelatin different concentrations of 0.1‚ 0.5‚ and 1 mg/ml were treated. After an incubation period of 24 h‚ the spent media were removed and the plate wells were washed with Phosphate buffered solution. Briefly‚ 50 IL of 2 mg/ml MTT and 150 IL culture medium were added to each well. The cells were incubated at 37 °C and 5% Co2 for 4 h. Then the media was discarded and dimethyl sulfoxide and Sorenson buffer was added to each well as solubilizer buffer. Finally‚ absorbance was read
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The PLGAh and PLGAh+Q carrier was tested by these assay for rate of apoptosis in the in Michigan cancer foundation (MCF-7) cell lines. The control was the normal cancer cell lines without any PLGAh and PLGAh-Q carrier. The PLGAh carrier was tested in assay in two different concentrations which was found above 1.5 µg and 3.0 µg. The summation of early and late apoptosis was just 8 % in concentration of
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Results and Discussion: A reaction involving two bond formation was employed for the preparation of 3‚5-disubstituted 1‚2‚4-triazole as nucleobase analog. The hydrazides 2a‚b were allowed to react with the heteroaryl substituted acetonitrile derivatives 1a-c using catalytic amount of K2CO3 and n-butanol as a solvent to afford the disubstituted 1‚2‚4-triazoles 3-7 bearing benzothiazole‚ benzotriazole‚ quinoloxymethyl and benziimidazole ring systems in moderate yields. The structures of compounds
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Santos‚ J. (1928). Stem and leaf structure of Tinospora rumphii Boerlage and Tinospora reticulata Miers. Philippine Journal of Science ‚ 35(2)‚ 187-208. • Twentyman‚ P. R.‚ & Luscombe‚ M. (1987). A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity. British Journal of Cancer‚ 56(3)‚ 279-285. C. Internet • "A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate (ab7910) | Abcam." Antibodies‚ Proteins‚ Kits and Reagents for Life Science
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3. Result and discussion 2.1 Physicochemical properties The formations of BPM I-III were confirmed by FT-IR‚ powder XRD and SEM studies. 2.1.1 Fourier transform infrared spectroscopy FT-IR spectra of BPM I-BPM III (Fig. 1a-1c) exhibited characteristic absorption bands of CTS ranges from 1637-1639 cm−1 which are attributed to amide I bands (C=O stretching mode along with an N–H deformation mode). Broad bands from 1130-978 cm−1‚ 1131-975 cm−1 and 1131-971 cm−1 were observed for phosphate stretching
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cardiotonics and alkaloids was observed. The high content of phenolic compounds happens to be interesting as there is a high probability polar extract from the leaves of this plant have antioxidant activity. Preliminary experiments with trypan blue and MTT with different concentrations of eleven fractions of L. arborea‚ showed four fractions exhibit cytotoxic activity (F6‚ F8‚ F9 and F10) in Jurkat and CHO-K1 cell lines. Antiproliferative
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Combination of flow with 3D cell culture improves metabolic competency in multiple cell types Lewis ME‚a* Gordon P‚a Tholozan F‚b Ciammaicella D‚c Vinci B‚c Przyborski S‚b Krajewska E‚a Darley K‚a Wilkinson JM a a. Kirkstall Ltd.‚ Sheffield UK. b. Reinnervate Ltd.‚ Durham UK. c. University of Pisa‚ Pisa‚ Italy. *Corresponding author. Kirkstall Ltd.‚ 40 Leavygreave Rd‚ Sheffield‚ UK‚ S3 7RD 1. Introduction • Growing cells in 2D under static conditions is the gold standard of cell culture. However
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joint ventures with Turkistan Global Services Sdn Bhd (TGS) to form a new entity‚ Malaysian Turkistan Travel Sdn Bhd (MTT)‚ in the purpose of exploring travel business opportunities in Turkistan. In this entity‚ MTSB holds 40% of the shares whereas the others held by TGS. However‚ there was no progress after the forming of MTT. He recalled that his friend‚ Norehan who had worked in MTT before. She told Mr. Idris that the company may have been established under fraudulent circumstances and the staff’s
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Daisy Lene M. Diomampo B.S-Pharmacy IV Articles related to Bignay Leaf Extract Bignay is a small‚ smooth‚ dioecious tree‚ 4 to 10 meters high. Leaves are shiny‚ oblong‚ 8 to 20 centimeters long‚ pointed at the tip‚ rounded or pointed at the base. Spikes are axillary or terminal‚ simple‚ and usually 5 to 15 centimeters long. Flowers are small and green. Male flowers are about 1.5 millimeters in diameter‚ borne on spikes‚ while the female flowers grow out on racemes. Fruit is fleshy‚ red‚ acid
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C A S E 3 Asian Journal of Case Research 4(S): 27 – 44 (2011) Flight of Funds MOHD NOOR ABU BAKARa‚ MARIATI NORHASHIMb* AND ABDUL HAMID MOHAMED GHOWSc ABSTRACT In 2006‚ Malaysian Travels Sdn Bhd‚ a subsidiary of a public listed company‚ purchased 40% equity in Turkistan Global Services Sdn Bhd on the basis of a project paper which outlined a potential travel business to exploit opportunities in Turkistan. Turkistan Global Services Sdn Bhd purported to have acquired the opportunity through a Memorandum
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