Several ATPase assays containing Pi‚ Mg2+ or no Mg2+‚ and DCCD were conducted where the reverse reaction -ATP hydrolysis into ADP and Pi- is used as an approximate measure of ATPase activity and dependence upon Mg2+‚ as well as the effects of inhibition by DCCD on Pi production. The ion gradient needed to power this ATPase will also be of importance as we determine what substrates the ETC preferentially oxidizes to produce the necessary H+ ion gradient. An oxygen consumption assay was employed with
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BAsic ReseARch PAPeR BAsic ReseARch PAPeR Autophagy 9:5‚ 1–16; May 2013; © 2013 Landes Bioscience Inhibition of the autophagic flux by salinomycin in breast cancer stem-like/progenitor cells interferes with their maintenance Wen Yue‚1‚2‚† Ahmed hamaï‚1‚2‚† Giovanni Tonelli‚1‚2 chantal Bauvy‚1‚2 Valérie Nicolas‚2‚3 hugo Tharinger‚2‚3 Patrice codogno1‚2 and Maryam Mehrpour1‚2‚* 1 † These authors contributed equally to this work. Keywords: breast cancer stem-like/progenitor cell
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platyloba EO showed stronger antioxidant activity than ascorbic acid and BHT using FRAP assay in this study. Pirbalouti et al.‚(2013) evaluated the antioxidant activity of methanol extracts of Heracleum lasiopetalum Boiss‚ Kelussia odoratissima Mozaff.‚ and E.platyloba DC. by three assays‚ including DPPH‚ FRAP‚ and TEAC. From all three assays‚ E. platyloba had the highest antioxidant activity. They showed that FRAP assay increased in the order of BHT ≥ E. platyloba. Different components of E.platyloba
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CPL Andy was an amour technician from 12 Forward Maintenance depot (12FMD) under 1st Army Maintenance Base (1 AMB). He was assigned to platoon 2 section 4. CPL Andy takes pride in producing work of quality and has been excellent throughout his service as an auto technician in 12 FMD. A meticulous and hardworking person who always set high standards for himself when it comes to work. He always ensures that repair results are of excellent quality and the deadline of the task is met. Even when faced
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available for the detection and definition of HLA specific antibodies and to interpret the results from the various assays in is important to understand the different principles underlying the assays as well as their relative strengths and weakness‚” (Fuggle & Martin‚ 2008‚ para. 5). Cell Based Assay According to Fuggle & Martin‚ (2008) Compliment Dependent Cytotoxicity (CDC) assay
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Oil Characterization 1 Oil Characterization 1 © 2003 AspenTech. All Rights Reserved. EA1031.31.05 05 Oil Characterization.pdf 2 Oil Characterization Workshop The petroleum characterization method in HYSYS converts laboratory analyses of condensates‚ crude oils‚ petroleum cuts‚ and coal-tar liquids into a series of discrete hypothetical components. These petroleum hypo components provide the basis for the property package to predict the remaining thermodynamic and transport properties necessary
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for all the assays was 8x which falls between an absorbance of 0.5-1.0 when measured at 405nm. On Figure. 1‚ it shows the variation of rate as a function of the weight of alkaline phosphatase. In the graph‚ the rate
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hydrogen peroxide and 20 mL of water. In the second beaker‚ a ¼ dilution of potato extract was made using 2 mL of potato extract and 6 mL of water. The third plastic beaker contained 8 mL of water. Using the first and second beaker the experimental assay was performed. Using forceps a small paper disk was dipped into the second beaker containing the potato catalase for exactly 5 seconds. It was next dried on a paper towel for 5 seconds‚ and finally placed at the bottom of the first beaker‚ which
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Rheumatoid arthritis is an autoimmune disease that can cause swelling‚ stiffness and pain of joints‚ primarily in an individual’s small joints in their hands and feet. An autoimmune disease is the result of antibodies from a person’s immune system attacking their own healthy cells. Many autoimmune diseases act in various different manners and one of the rare similarities is the ability to clinically diagnose a disease based on the presence of autoantibodies in a serum. When these autoantibodies begin
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Experiment 4: Enzyme Kinetics. Results/Discussion Week 1 Part A: Table 1. Enzyme activity for each assay of 4-nitroaniline formation. Rate of 4-nitroaniline formation Name of trial Abs/sec Abs/min M/min mol/min µmol/min #1 0.00003 0.0018 2.05x10-7 2.15 x10-10 2.15 x10-4 # 2 0.00010 0.0060 6.81x10-7 7.15x10-10 7.15x10-4 # 3 0.00020 0.0120 1.36x10-6 1.43x10-9 1.43x10-3 # 4 0.00030 0.0180 2.00x10-6 2.10x10-9 2.10x10-3
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