Laboratory Exercise #3 Measuring Protein in Solution Abstract The purpose of this lab was to learn about the Biuret assay reaction to determine if it can detect proteins and amino acids; also‚ to understand the process of “salting out” proteins and how to determine the amount of protein in a solution. In order to do so‚ egg white and ammonium sulfate were mixed on ice and then put into the centrifuge. After PBS was added‚ the amount of protein could then be determined. After that‚ 14 test tubes
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The Affects of pH on Peroxidase Enzyme Claire Bell Biology 1130 Jazmen Myers Section 36 Abstract Peroxidase is a turnip enzyme; it is used in the oxidation of hydrogen peroxide to lower activation energy‚ speeding up the reaction. The activity of peroxidase is highly dependent on its environment and most importantly the pH level. Peroxidase has been the focus of many recent studies and is believed to possibly reduce swelling among other things. We conducted an experiment testing
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Organ transplantation is a treatment of choice to replace damaged organs through the intervention of a donor. There are several types of donors according to their provenance. The donor of organs between two identical twins is called isograft‚ when donor and recipient is the same individual is called autograft‚ donor from the same species but different person is called allograft and donor coming from different species with the recipient is called xenograft (Fernandez‚ 2012). Apart of the above
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which indicates that the measurement is not accurate. C) Comparison of the Spot vs OD Assays 1. (4 points) Compare and comment on the concentration values calculated using the spot method vs the OD method with the spectrophotometer. Which do you think is more accurate? Why? Although I can not compare the concentration values between the spot method and the OD method‚ due to the pipetting error in the spot assay‚ the OD method with the spectrophotometer was more accurate due to the smaller possibilities
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Figure 3: Incorrect Ethanol Well Assay Results This solution is still green because our group did not do it correctly. Thus different measures were used. Table 3: Ethanol Well Plate Assay Results from Figure 4 Sample Concentration (M) A 2.4 B 2.4 C 0.0 D 1.2 E 2.4 Figure 4: Correct Ethanol Well Plate Assay Results The column on the left for this figure 3 and the first well at the bottom of the second column are the
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three species of bacteria were incubated under different salt concentrations‚ and six were used in two separate procedures where incubation took place at various temperatures. These organisms were classified based on the observations made after the assays conducted. INTRODUCTION For the most primordial forms of life‚ osmotic stress and variations in temperature‚ when not fatal‚ were harmful for their growth. Nowadays‚ however‚ microorganisms can be found almost anywhere we can think of‚ independently
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amplification in the nuclei and provides an objective count of the genes and chromosomes on a cell-by-cell basis. However‚ it requires a fluorescence microscope and special training for interpretation. The cost of either of the 2 FDA-approved FISH assays (just reagents) is almost $100‚ with considerable cost added by the need to purchase a fluorescence microscope and filters. It also may be difficult to visualize the morphologic features of the tumor cells and to separate in situ from invasive
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Pre-Lab 1 Assignment Quantitative Analysis of Biomolecules Biochemical analysis involves the characterization of biomolecules within a sample using appropriate laboratory techniques. There are two principal approaches: 1. Qualitative analysis – where a sample is analyzed to determine whether a biomolecule is present or absent. As an example‚ a blood sample might be analyzed for a specific antibody or a bacterial cell might be probed for a nucleic acid sequence. 2. Quantitative analysis
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Centrifuge tube B at 12000 RPM for 30 min to get mitochondrial pellet and a supernatant. (Balance test tubes before centrifugation). - Resuspend the pellet (to avoid clumps) using cold stirring glass rod. Add one drop of assay buffer. Continue breaking clumps and adding assay buffer until 5ml buffer is added. - Mix the suspension and transfer to a precool 15 ml test tube M. - Prepare slide using suspension from M tube and adding Janus Green B dye to it. Cover using cover slip. - Add 1 ml of
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Chlorophylls absorb all the visible light wavelengths below 510nm and a fraction of the visible light wavelengths above 625nm. Explaining why grass appears green‚ the chlorophyll is green because it reflects the green wavelengths the most. 3. NADI ASSAY RESULTS – You may adjust the table columns widths to your Tube Initial color & final color Format: (initial/final) Designate each tube as a “CONTROL” or “EXPERIMENTAL” For ‘CONTROL’ tubes – explain the purpose for control For ‘EXPERIMENTAL’
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