Introduction: Cytochrome c oxidase plays a vital role in cellular respiration by accepting e- from cytochrome c and transferring them to an acceptor oxygen molecule in the final step of electron transfer chain. Carbon monoxide and cyanide are few of the inhibitor of this enzyme. 4 Fe2+ -cyt c + 8H+ + O2 4 Fe3+ -cyt c + 2H2O + 4H+ [out] Cytochrome c oxidase locates to the inner membrane which separates the mitochondrial matrix from the intermembrane space. However‚ Potato tubes can
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It appears that the majority of HSCs are CD34+‚ as shown by transplantation and xenograft repopulation assays‚ however most of these cells are lineage-restricted progenitors and HSCs are therefore rare. Enrichment of HSCs seems reliant on CD45RA‚ Thy1and CD38 expression. Further study into the role of each of these factors in HSC differentiation is required. In this study a range of assays were carried out in an attempt to identify and separate HSCs from MPPs. Recently it was shown that a depletion
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In the known HCV status experiments‚ I ran the assay four times for each sample and then used the mean of the four to evaluate the levels of HCV. I did this because I wanted to get accurate results. I believe if you repeat the experiment many times‚ the mean of the results will get closer to the true mean. During my science classes‚ we gained knowledge through repeated observation. For example‚ we used error bar where we are trying to compare experiment with controls in Biology. Hepatitis C virus
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Bradford Assay method. In the first part‚ egg white underwent the addition of a weak acid‚ disruption of cell membrane‚ addition of ammonium sulfate and centrifugation. The product obtained was not weighed because of its relatively small amount and was considered a failure by the experimenters. In the second part of the experiment‚ the protein concentration of the product acquired from the first part was calculated to be 5.1 mg/mL using the Warburg-Christian method. In the Bradford assay method‚
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clumps of 200-500nm. A MTT test also undertook to test the cytotoxicity on cells‚ figure 10b shows the cell population decreased for both filtered with particle size ranging 30-600 nm and unfiltered ink 40-970nm‚ a larger decrease in cell numbers for filtered ink signifying singular tattoo nanoparticles has a negative effect on the cells. Similar test on fibroblast was taken by Biolip 27 ink also showed a similar trend‚ it showed a reduction of fibroblast by 47% after a MTT test was taken ruminating
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Lab Report 8 April 15th Exercise 8: Chemical and Physical Processes of Digestion Lab Report Questions Activity 1 What is the difference between the IKI assay and Benedict’s assay? IKI assay detects the presence of starch‚ and the Benedict assay tests for the presence of reducing sugars as well as IKI turns blue black whereas Benedict is a bright blue that changes to green to orange to reddish brown with increasing levels of maltose What was the purpose of tubes #1 and #2? Why are they
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ABTS assay was run according to the method of Arnao et al. 2001 with some modification. Equal volumes of 7mM ABTS and 35mM K2S2O8 (potassium per sulphate) were mixed and incubate for 24 hours. 1200 μl of the sample‚ from each concentration i.e. 50- 500 μg/ml
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MTT (Set 5 with answer) 1) The following is selected information from the records of Ray‚ Inc.: Purchases of raw materials $ 6‚000 Raw materials‚ beginning 500 Raw materials‚ ending 800 Work-in-process‚ beginning 0 Work-in-process‚ ending 0 Cost of goods sold 12‚000 Finished goods‚ beginning 1‚200 Finished goods‚ ending 1‚400 What is the total amount of conversion costs? a. $5‚500 b. $5‚900 c. $6‚100 d. $6‚500 ANSWER: Choice "d" is correct. Conversion costs (labor and overhead)
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SEROLOGICAL‚ CULTURAL AND MOLECULAR DETECTION OF BRUCELLA INFECTION IN BREEDING BULLS A THESIS SUBMITTED TO THE ANAND AGRICULTURAL UNIVERSITY IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF Doctor of Philosophy IN VETERINARY MICROBIOLOGY BY AMIT N. KANANI M. V. Sc. DEPARTMENT OF VETERINARY MICROBIOLOGY COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND-388001 (GUJARAT) 2007 Reg. No. 04-05194-2001 Dr. J. H. Purohit
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DETERMINING THE GLUCOSE CONTENT OF AND ORANGE USING GOD-PAP ASSAY Every fruit has a sweet taste only that some are sweeter than others. The sweetness of most fruits come from its sugar content and these sugars that the fruits contain are known as invert sugars. In this experiment‚ an orange was used. An orange which is an excellent source of vitamin C gets its sweetness from natural sugars which are sucrose‚ glucose and fructose (livestrong.com). In this experiment the concentration of glucose in
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