Enzyme Study Questions 1. Definitions/terminology: o enzyme: a protein molecule that catalyzes chemical reactions without itself being destroyed or altered o catalyst: a substance that increases the rate of a chemical reaction but is not consumed or changed by it. o substrate: a substance upon which the enzyme acts. o denaturation: the partial or total alteration of the structure of a protein without change in covalent structure by the action of certain
Premium Enzyme
refinery process of producing green chemicals (Ponnambalam et al.‚ 2011). In screening for the cellulolytic fungi‚ several qualitative display of cellulolytic such congo red clearing zone assay‚ gel diffusion assay and dyed congo red filter paper clearing zone assay can be used. In this screening‚ congo red clearing zone assay is performed on 9 unknown fungi isolates on CMC media. Cellulose degradation and its subsequent utilizations are important for global carbon sources (Ponnambalam et al.‚ 2011). The
Premium Cellulose Enzyme Fungus
dose-dependent manner. The antioxidant activity was correlated with the amount of total phenolics present in the respective extracts in each assay. 50% acetone proved to be the most efficient solvent for extraction of antioxidants from mahua as the related extract contained the highest amount of phenolic compounds and also exhibited the strongest antioxidant capacity in all the assays used‚ while ethanol was the inefficient solvent for the extraction of phenolic compounds‚ Whereas ethanol and distilled water have
Premium Protein Solvent Oxygen
The antioxidant activity of plant materials or extracts is known to be associated with polyphenols and their chemical structures that contain hydroxyl groups acting as the primary antioxidant (Jun et al.‚ 2014). 2.5.2. DPPH• scavenging activity assay DPPH• scavenging activity was measured according to a previously published method (Brand-Williams‚ Cuvelier‚ & Berset‚ 1995) with some modifications. One half milliliter of each extract solution was added to 1 ml of a freshly prepared 0.1 mM
Premium Oxygen Acetic acid Carboxylic acid
Phytochemical characterization & antioxidant activity of mangrove plant Sonneratia caseolaris Submitted by: Kumari Priyanka Regd no.-0901106090 Sem-7th‚ Biotechnology
Premium Antioxidant
For QCM-D experiments‚ liposomes of 50 nm were prepared at 1 mg/mL concentration in Buffer B (Citrate 10mM‚ 100mM NaCl‚ and 0.5 mM EGTA‚ pH 4.6). All liposomes were used within 24h and concentration of the liposomes was measured using phospholipid assay kit (Sigma-Aldrich). Giant Unilamellar Vesicles (GUVs):
Premium Chemistry Water Temperature
lysing methods: Brad Ford Assay‚ SDS-PAGE‚ affinity purification‚ lysozyme treatment‚ nickel column‚ sonication‚ and flavin reeducate activity assay. His-tagged protein is expressed in E. coli from pGhis‚ with the T7 RNA lac repressor induction system.5 Sonication is used‚ it’s why bacteria are lysed. The tag allows purification for ion affinity chromatography‚ following a gel filtration column‚ defined as removing ions and other small molecules. Where then‚ the Bradford assay was made use of to identify
Premium Bacteria Escherichia coli DNA
Therefore‚ post-assay samples were sent for Mass Spectrometry analysis. The Mass Spectrometry analysis (High Resolution Electro-Spray Ionization Mass Spectrometry‚ HR-MS) came back positive with a clear peak (retention time = 5.172‚ m/z = 508.3) which correlates well with
Premium Protein Oxygen Amino acid
Five concentrations of TE will be tested‚ the highest of which will be the highest concentration revealed by the embryotoxicity assay as non-lethal and non-teratogenic. The other concentrations will be determined by using 2 as a spacing factor. Six embryos will be exposed to each of the test concentrations in 24-well plates. The embryos will be incubated at 26 ºC for 48 h in a
Premium DNA Gene Bacteria
2 BASIC PRINCIPLE 3 TYPES OF PCR 4 qPCR STEPS 4 ONE-STEP OR TWO-STEP REACTION 6 Overview of qPCR and qRT-PCR components 6 REAL TIME PCR SYSTEM: 7 SOFTWARES FOR DATA ANALYSIS AND PRIMER DESIGNING 8 STANDARD REAL-TIME PCR PROTOCOL 9 ASSAY DESIGN 9 2. Nucleic acid purification 9 3. Reverse transcription 9 4. Controls and normalization 9 5. Standard curve evaluation of efficiency‚ sensitivity‚ and reproducibility 9 Real-Time PCR Fluorescence Detection Systems 12 DNA-Binding
Premium Polymerase chain reaction